Densely Packed Microgoblet Laser Pairs for Cross‐Referenced Biomolecular Detection

Abstract

Microgoblet laser pairs are presented for cross‐referenced on‐chip biomolecular sensing. Parallel readout of the micro­lasers facilitates effective mutual filtering of highly localized refractive index and temperature fluctuations in the analyte. Cross‐referenced detection of two different types of proteins and complete chemical transducer reconfiguration is demonstrated. Selective surface functionalization of the individual lasers with high spatial accuracy is achieved by aligned microcontact stamping.

Self-limiting multiplexed assembly of lipid membranes on large-area graphene sensor arrays

“…5 As the resulting membrane stacks exhibit superior properties with regard to minimizing non-specific adhesion of proteins, have high biocompatibility, and can be tuned in function by admixing of functionalized lipids or small molecules as cargo, they found wide application ranging from biological experiments on mast cells, 6,7 drugdelivery experiments, 8 bio-sensing 9 and functionalization of sensor devices. [10][11][12][13] Recently, we reported the successful generation of functional biomimetic lipid patches on graphene by L-DPN. 14 Interestingly, we found a high mobility and rapid selforganisation of the lipid membrane on the graphene.…”

Making the best of it: nitroxide-mediated polymerization of methacrylatesviathe copolymerization approach with functional styrenics

“…The first methacrylic ester we investigated was logically methyl methacrylate, which is probably the most ubiquitously used one, with applications spanning from glass replacement 62 or contact lenses 63 to microdevices. 64 The simplest way to perform NMP is to mix an alkoxyamine with a given monomer and to raise the temperature in the absence of oxygen. This is a so-called bulk polymerization.…”

“…S3-10 †). Concerning PMMA, being a material used for many optical applications 63,64 or simply when transparence in the final object is required along its particular properties, 62,[72][73][74] it is important to ascertain that its optical properties are not strongly impacted by the presence of the controlling comonomer. As a relevant reference sample, we synthesized a PMMA sample by ATRP (PMMA ATRP ), which does not necessitate the introduction of a controlling comonomer.…”

Robust label-free biosensing using microdisk laser arrays with on-chip references

“…Note that the detection limit of 104 ng/ml is the best value so far obtained for active WGM sensors, outperforming the 134 ng/ml demonstrated in previous experiments, for which photo-bleaching had to be avoided by small pump powers. These experiemnts were also based on rather complex fabrication techniques, involving reflow processes to smoothen the WGM surfaces as well as aligned micro-contact stamping for local deposition of passivation layers [25]. Our present results can be further improved, e.g., through optimized surface functionalization with higher density of receptor molecules, through increasing the overlap of the resonant modes with the surrounding by optimizing resonator dimensions [4], or through advanced data acquisition and processing techniques which are subject of further studies.…”

“…This approach, however, is only applicable to relatively flat structures of submicron height and is not suitable for high quality-factor resonators on elevated pedestals, for which spin-coating of a thick homogeneous passivation layer is challenging. For elevated resonators on pedestal structures, conformal coating of the reference device surface by a phospholipid film was demonstrated [25]. The viability of this approach was shown in a referenced sensing experiment, relying on a pair of size-mismatched adjacent microlasers, where one laser was functionalized while the other was passivated as a reference.…”