Densitometric and videodensitometric TLC determination of timolol and betaxolol in ophthalmic solutions

Hopkała, Hanna; Pomykalski, Arkadiusz; Mroczek, Tomasz; Ostęp, Marzena

doi:10.1556/jpc.16.2003.4.6

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Cited by 5 publications

(1 citation statement)

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“…Analytical methods for betablockers determination in biological samples include liquid chromatography with UV or fluorescence detection [18][19][20], TLC [21] or voltammetry [22]. Due to the complex matrix of biological samples, pretreatment procedures such as LLE, or SPE are required for β-blockers analysis [23].…”

Section: Introductionmentioning

confidence: 99%

On-line coupling of Micro-Extraction by Packed Sorbent with Sequential Injection Chromatography system for direct extraction and determination of betaxolol in human urine

Šrámková

Chocholouš

Sklenářová

et al. 2015

Talanta

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Section: Introductionmentioning

confidence: 99%

On-line coupling of Micro-Extraction by Packed Sorbent with Sequential Injection Chromatography system for direct extraction and determination of betaxolol in human urine

Šrámková

Chocholouš

Sklenářová

et al. 2015

Talanta

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Betaxolol: A comprehensive profile

Alwadei

Bakheit

Abdel‐Aziz

et al. 2021

Profiles of Drug Substances, Excipients and Related Methodology

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Quantitative ultraviolet measurements on wetted thin-layer chromatography plates using a charge-coupled device camera

Lancaster

Goodall

Bergström

et al. 2008

Journal of Chromatography A

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This paper presents the first study of the UV imaging of spots on thin-layer chromatographic plates whilst still wet with solvent. Imaging of spots of benzophenone during and after development was carried out using a charge-coupled device camera. Limits of detection were found to be 5ng on a wetted plate and 3ng for a dry plate and the relationship between peak area and sample loading was found to be linear in the low nanogram range over an order of magnitude for both wet and dry modes with r(2) values>0.99. It was found that UV measurements on wet glass-backed plates suffer from low sensitivity; however, the use of aluminium-backed plates gave increased sensitivity. The apparent absorption coefficient epsilon(app) of 10AUm(2)g(-1) at 254nm is consistent with reflection of the light from the aluminium surface with a double pass through the sorbent layer, and suggests that use of aluminium-backed plates should enable monitoring of separations by UV absorbance during TLC development.

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Densitometric and videodensitometric TLC determination of timolol and betaxolol in ophthalmic solutions

Cited by 5 publications

References 13 publications

On-line coupling of Micro-Extraction by Packed Sorbent with Sequential Injection Chromatography system for direct extraction and determination of betaxolol in human urine

On-line coupling of Micro-Extraction by Packed Sorbent with Sequential Injection Chromatography system for direct extraction and determination of betaxolol in human urine

Betaxolol: A comprehensive profile

Quantitative ultraviolet measurements on wetted thin-layer chromatography plates using a charge-coupled device camera

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