Densonucleosis of bloodsucking mosquitoes

Buchatsky, L.

doi:10.3354/dao006145

Cited by 38 publications

(33 citation statements)

References 7 publications

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“…A baculovirus may prove useful in the control of Culex mosquitoes (Becnel et al 2001). The Aedes densonucleosis virus (family Parvoviridae, genus Brevidensovirus, AeDNV) was originally isolated in the Ukraine (Lebedeva et al 1972) and also has potential as a biological control agent (Buchatsky 1989). AeDNV has been extensively characterized with respect to its host range and has been found to be highly speciÞc to mosquitoes of the Aedes and Culex genera.…”

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confidence: 99%

<I>Aedes aegypti</I> Densonucleosis Virus Amplifies, Spreads, and Reduces Host Populations in Laboratory Cage Studies

Suchman¹,

Piper

Valdez

et al. 2009

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Aedes densonucleosis virus (family Parcoviridae, genus Brevidensovirus, AeDNV) is a mosquito pathogen that increases Aedes aegypti larval mortality and reduces adult life span. We conducted three laboratory population cage trials, each lasting 16-25 wk. We tested two broad hypotheses. First, Ae. aegypti raised in containers seeded with 10(8) AeDNV genome equivalents/ml (geq/ ml), a concentration feasible for field application, increase AeDNV to concentrations that cause significant adult and larval mortality. Second, infected female mosquitoes disperse AeDNV to uninfected larval habitats. In hypothesis 1, we addressed the rate at which infected larvae secrete virus, how AeDNV titers change in seeded containers over time, whether AeDNV decays over time, and whether AeDNV exposed populations are reduced. In hypothesis 2, we monitored AeDNV concentrations in novel containers after oviposition by infected females. Both hypotheses were supported. Larvae increased AeDNV, secreting virus at a rate of 2.14 x 10(6) geq/larva/d when exposed to 10(8) geq/ml. AeDNV titers reached an asymptote of 10(10) geq/ml by week 10 in seeded containers. AeDNV decayed by 1 log every 4 d as indicated by a reduction in larval mortality. Adult population size was reduced in treated populations. Infected females dispersed AeDNV to novel containers, with titers reaching 10(8) geq/ml. The parameters were used in a Leslie-Lewis matrix model. This model predicted that AeDNV negatively affects Ae. aegypti densities and population structure and thus vectorial capacity.

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<I>Aedes aegypti</I> Densonucleosis Virus Amplifies, Spreads, and Reduces Host Populations in Laboratory Cage Studies

Suchman¹,

Piper

Valdez

et al. 2009

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“…The type species of the genus Brevidensovirus is Aedes aegypti densovirus (AaeDNV) (Buchatsky., 1989). The genus contains several other isolates including Aedes albopictus densovirus (AalDNV) [this species contains to date three distinct viruses from C6/36 cells all identified as AalDNV, we shall refer to these in this paper as AalDNV-1 (identified by Jousset et al, 1993;Boublik et al, 1994), AalDNV-2 (identified by Chen et al, 2004) and AalDNV-3 (identified by Paterson et al, 2005)].…”

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Isolation and characterization of the full coding sequence of a novel densovirus from the mosquito Culex pipiens pallens

Zhai¹,

Lv²,

Sun³

et al. 2008

Journal of General Virology

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During an investigation of arboviruses in China, a novel densovirus (DNV) was isolated from the adult female Culex pipiens pallens. The virus, designated Culex pipiens pallens densovirus (CppDNV), caused cytopathic effect in C6/36 cells. The virus particles were icosahedral, non-enveloped and had a mean diameter of 24 nm. The complete coding region of CppDNV was found to be 3335 nt and it contained three open reading frames (ORFs). CppDNV shares 82-93 % identical nucleotides with isolates of the Aedes albopictus densovirus [isolates AalDNV-1, AalDNV-2 (C6/36 DNV) and , Aedes aegypti densovirus (AaeDNV) and Haemagogus equines densovirus (HeDNV). The nucleotide sequence identity among CppDNV isolates exceeds 98 %. Phylogenetic trees based on non-structural (NS1 and NS2) and capsid (VP) genes show that CppDNV clustered with the species AaeDNV and represents a novel variant of this species within the genus Brevidensovirus.Densoviruses (DNVs) are classified as members of the genera Densovirus, Iteravirus, Brevidensovirus and Pefudensovirus (subfamily Densovirinae) within the family Parvoviridae. They represent a group of non-enveloped viruses, with single-stranded DNA genomes, encapsidated within icosahedrally arranged viral particles. Their host range is limited to a few closely related invertebrates, particularly insects. However, some DNVs also infect and multiply in shrimps. Members of the genera Densovirus and Iteravirus infect lepidopterans (Tijssen & Arella., 1991). Members of the genus Brevidensovirus infect mosquitoes (Ward et al., 2001), while members of the genus Pefudensovirus infect cockroaches. Members of the genera Densovirus and Pefudensovirus have ambisense genomes that are 5.5-6 kb long. Their structural and non-structural proteins are encoded from separate strands, while those of the genera Iteravirus and Brevidensovirus are monosens (encoded from the same strand) and are~5 and 4 kb long, respectively (Jousset et al., 1993;O'Neill et al., 1995;Fauquet et al., 2005).The identification of DNVs in various mosquito cells and wild mosquitoes suggests that the brevidensoviruses have a widespread distribution (O'Neill et al., 1995). The type species of the genus Brevidensovirus is Aedes aegypti densovirus (AaeDNV) (Buchatsky., 1989). The genus contains several other isolates including Aedes albopictus densovirus (AalDNV) [this species contains to date three distinct viruses from C6/36 cells all identified as AalDNV, we shall refer to these in this paper as AalDNV-1 (identified by Jousset et al., 1993;Boublik et al., 1994), AalDNV-2 (identified by Chen et al., 2004) and AalDNV-3 (identified by Paterson et al., 2005)]. Culex pipiens densovirus (CpDNV) was isolated from Culex pipiens larvae; however, it is genetically related to the type speciesThe GenBank/EMBL/DDBJ accession numbers for the sequences reported in this paper are EF579756-EF579771.Supplementary tables are available with the online version of this paper. (2008( ), 89, 195-199 DOI 10.1099 Here we report the isolation and characterization of a ...

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“…caspius, and to a lesser extent Culex pipiens. 10 AaPV is highly pathogenic for Ae. aegypti, killing up to 95% of larvae after oral infection.…”

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A mosquito densovirus infecting Aedes aegypti and Aedes albopictus from Thailand.

Kittayapong

Baisley

O'Neill

1999

The American Journal of Tropical Medicine and Hygiene

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Abstract. A previously undescribed mosquito densovirus was detected in colonies of Aedes aegypti and Ae. albopictus from Thailand, using a polymerase chain reaction (PCR)-based assay. Phylogenetic analysis of this virus showed it to be most closely related to ADNV isolated from Russian Ae. aegypti. Both Aedes species were susceptible to oral infection with the Thai-strain virus. Larval mortality for Ae. albopictus was higher (82%) than for Ae. aegypti (51%). Aedes aegypti were able to transmit the virus vertically to a high (58%) proportion of G 1 progeny, and the virus was maintained persistently for up to six generations. A PCR survey of adult Ae. aegypti and Ae. albopictus in Thailand indicated that only Ae. aegypti are infected in the field, with an overall prevalence of 44%. Densovirus infection in adult Ae. aegypti showed distinct seasonal variation. The Thai strain densovirus may play a role in structuring Ae. albopictus and Ae. aegypti populations in nature.Insect densoviruses are small, autonomous, non-enveloped DNA viruses that belong to the family Parvoviridae. They are characterized by the presence of inverted terminal repeat sequences and the separate encapsidation of complementary single-stranded DNA. Two subgroups of insect densoviruses have been recognized on the basis of lepidopteran isolates. 1Subgroup I densoviruses have a 6-kb genome, code their structural and nonstructural proteins from separate strands, and infect all insect cells except those of the midgut. Subgroup II densoviruses have a smaller genome, approximately 4.9 kb, code all their proteins from one strand, and infect only insect midgut cells. Mosquito densoviruses do not easily fit into this classification scheme, and as such form a third subgroup. They are characterized as having a 4-kb genome and infecting all tissues of their insect hosts.2 While Subgroup I and II densoviruses typically encapsidate both plus and minus DNA strands with equal frequency, some mosquito densoviruses encapsidate primarily minus strands. 3,4 To date, five mosquito densoviruses have been described. The first, ADNV, was isolated from Aedes aegypti larvae, 3,5 while the second, AaPV, was recovered from an Ae. albopictus (C6/36) cell line. 4,6,7 Genomic sequence data have been obtained for both these densoviruses. A third was observed by electron microscopy of an Ae. pseudoscutellaris (AP-61) cell line. 8 More recently, two additional mosquito densoviruses (TaDNV and HeDNV) were detected using a polymerase chain reaction (PCR) assay in infected Toxorhynchites amboinensis (TRA-284) and Haemagogus equinus (GML-HE-12) cell lines, respectively.2 The PCR products from both viruses were sequenced, and infection was further confirmed by electron microscopy and indirect fluorescent antibody tests.Mosquito densoviruses are believed to be maintained in nature primarily by horizontal transmission, although transovarial and venereal transmission have also been recorded. 2,9 These viruses are often pathogenic to their arthropod hosts. ADNV was found to efficiently ki...

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Densonucleosis of bloodsucking mosquitoes

Cited by 38 publications

References 7 publications

<I>Aedes aegypti</I> Densonucleosis Virus Amplifies, Spreads, and Reduces Host Populations in Laboratory Cage Studies

<I>Aedes aegypti</I> Densonucleosis Virus Amplifies, Spreads, and Reduces Host Populations in Laboratory Cage Studies

Isolation and characterization of the full coding sequence of a novel densovirus from the mosquito Culex pipiens pallens

A mosquito densovirus infecting Aedes aegypti and Aedes albopictus from Thailand.

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