ObjectivesThe objective of this current survey was to assess both the occurrence and severity of pre‐ and post‐endodontic pain among female individuals, distinguishing between those with anemia and those without.Materials and MethodsIn the current study, we included a total of 60 women with anemia (hemoglobin < 11) and those without anemia (hemoglobin > 13). We recorded the occurrence and severity of pain before and at 24, 48, and 72 h following root canal treatment using a visual analog scale (VAS) ranging from 0 to 10. Additionally, we collected data on patient age, tooth type, as well as pulpal and periapical conditions. Statistical analysis was carried out using two‐way ANOVA, pairedt‐tests, and Pearson correlation coefficient. The significance level for statistical tests was set at p ≤ .05.ResultsThe incidence of preoperative pain in both anemic and non‐anemic patients was 80%. The total incidence of post‐operative pain was recorded as 71.7% (82.2% in anemic and 61.1% in non‐anemic women). Compared with the pretreatment pain incidence and intensity, the values increased in 24 h but declined in 48 h and 72 h after treatment. Considering the posttreatment pain intensity, the mean values were higher in the three time intervals (24, 48, and 72 h) in anemic patients. Pulpal and periapical status, in contrast to age and tooth type, significantly contributed to the intensity of posttreatment pain.ConclusionRegarding the importance of pain phenomenon in human life, it is recommended to consider anemia as an important risk factor for post‐endodontic pain. Early diagnosis and analgesic treatment interventions in anemic females, alongside the pain control during root canal treatment, could promote the patient's satisfaction and quality of care.