Dental and Nondental Stem Cell Based Regeneration of the Craniofacial Region: A Tissue Based Approach

Hughes, Declan; Song, Bing

doi:10.1155/2016/8307195

Cited by 18 publications

(19 citation statements)

References 116 publications

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“…In recent years, DPSCs are proved to be a promising choice in dentin and bone regeneration. The ability to manipulate the stem cells accurately to a desired cell lineage remains a much pursued goal of research [ 41 ]. Given the negative role of RA in mineralization, we propose that blocking RA signal may be an effective method for dentin regeneration from DPSCs.…”

Section: Discussionmentioning

confidence: 99%

Retinoic Acid Signal Negatively Regulates Osteo/Odontogenic Differentiation of Dental Pulp Stem Cells

Wang

et al. 2020

Stem Cells International

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Retinoic acid (RA) signal is involved in tooth development and osteogenic differentiation of mesenchymal stem cells (MSCs). Dental pulp stem cells (DPSCs) are one of the useful MSCs in tissue regeneration. However, the function of RA in osteo/odontogenic differentiation of DPSCs remains unclear. Here, we investigated the expression pattern of RA in miniature pig tooth germ and intervened in the RA signal during osteo/odontogenic differentiation of human DPSCs. Deciduous canine (DC) germs of miniature pigs were observed morphologically, and the expression patterns of RA were studied by in situ hybridization (ISH). Human DPSCs were isolated and cultured in osteogenic induction medium with or without RA or BMS 493, an inverse agonist of the pan-retinoic acid receptors (pan-RARs). Alkaline phosphatase (ALP) activity assays, alizarin red staining, quantitative calcium analysis, CCK8 assay, osteogenesis-related gene expression, and in vivo transplantation were conducted to determine the osteo/odontogenic differentiation potential and proliferation potential of DPSCs. We found that the expression of RARβ and CRABP2 decreased during crown calcification of DCs of miniature pigs. Activation of RA signal in vitro inhibited ALP activities and mineralization of human DPSCs and decreased the mRNA expression of ALP, osteocalcin, osteopontin, and a transcription factor, osterix. With BMS 493 treatment, the results were opposite. Interference in RA signal decreased the proliferation of DPSCs. In vivo transplantation experiments suggested that osteo/odontogenic differentiation potential of DPSCs was enhanced by inversing RA signal. Our results demonstrated that downregulation of RA signal promoted osteo/odontogenic differentiation of DPSCs and indicated a potential target pathway to improve tissue regeneration.

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Section: Discussionmentioning

confidence: 99%

Retinoic Acid Signal Negatively Regulates Osteo/Odontogenic Differentiation of Dental Pulp Stem Cells

Wang

et al. 2020

Stem Cells International

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“…The most important purpose of tissue engineering is to create therapeutic substitutes for regenerating tissues and organs. In dentistry and maxillofacial surgery, the reconstruction of critical-size mandibular or alveolar bony defects remains a challenge; thus, bone regeneration using cell-seeded scaffolds has been investigated [ 1 , 2 , 3 , 4 , 5 , 6 ]. The ideal requirements that a scaffold should have to improve bone healing are high porosity and an adequate pore size to facilitate cell seeding and diffusion of cells and nutrients; the capacity to transport nutrients, oxygen, and metabolites.…”

Section: Discussionmentioning

confidence: 99%

“…Tissue engineered-based approaches represent an important challenge in craniofacial reconstruction [ 1 , 2 , 3 ]. In fact, the main limitation of maxillofacial surgery is the ability to achieve the regeneration of hard and soft tissues lost in cases of trauma, disease, or medical issues.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Poly(Lactic-co-glycolic) Acid Alone or in Combination with Hydroxyapatite on Human-Periosteal Cells Bone Differentiation and in Sinus Lift Treatment

et al. 2017

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Most recent advances in tissue engineering in the fields of oral surgery and dentistry have aimed to restore hard and soft tissues. Further improvement of these therapies may involve more biological approaches and the use of dental tissue stem cells in combination with inorganic/organic scaffolds. In this study, we analyzed the osteoconductivity of two different inorganic scaffolds based on poly (lactic-co-glycolic) acid alone (PLGA-Fisiograft) or in combination with hydroxyapatite (PLGA/HA-Alos) in comparison with an organic material based on equine collagen (PARASORB Sombrero) both in vitro and in vivo. We developed a simple in vitro model in which periosteum-derived stem cells were grown in contact with chips of these scaffolds to mimic bone mineralization. The viability of cells and material osteoconductivity were evaluated by osteogenic gene expression and histological analyses at different time points. In addition, the capacity of scaffolds to improve bone healing in sinus lift was examined. Our results demonstrated that the osteoconductivity of PLGA/HA-Alos and the efficacy of scaffolds in promoting bone healing in the sinus lift were increased. Thus, new clinical approaches in sinus lift follow-up should be considered to elucidate the clinical potential of these two PLGA-based materials in dentistry.

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“…Generally, the principles of tissue engineering are based on three elements, including stem cells with multi-lineage differentiation potential, scaffolds as carriers for stem cells, and bioactive molecules inducing differentiation [ 93 ]. Dental MSCs are regarded as ideal cells for dental tissue engineering since they possess a shared embryological origin with craniofacial tissue [ 94 ].…”

Section: Dental Msc-based Therapy For Dental Diseasesmentioning

confidence: 99%

Dental Tissue-Derived Human Mesenchymal Stem Cells and Their Potential in Therapeutic Application

Gan

Liu

Cui

et al. 2020

Stem Cells International

101

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Human mesenchymal stem cells (hMSCs) are multipotent cells, which exhibit plastic adherence, express specific cell surface marker spectrum, and have multi-lineage differentiation potential. These cells can be obtained from multiple tissues. Dental tissue-derived hMSCs (dental MSCs) possess the ability to give rise to mesodermal lineage (osteocytes, adipocytes, and chondrocytes), ectodermal lineage (neurocytes), and endodermal lineages (hepatocytes). Dental MSCs were first isolated from dental pulp of the extracted third molar and till now they have been purified from various dental tissues, including pulp tissue of permanent teeth and exfoliated deciduous teeth, apical papilla, periodontal ligament, gingiva, dental follicle, tooth germ, and alveolar bone. Dental MSCs are not only easily accessible but are also expandable in vitro with relative genomic stability for a long period of time. Moreover, dental MSCs have exhibited immunomodulatory properties by secreting cytokines. Easy accessibility, multi-lineage differentiation potential, and immunomodulatory effects make dental MSCs distinct from the other hMSCs and an effective tool in stem cell-based therapy. Several preclinical studies and clinical trials have been performed using dental MSCs in the treatment of multiple ailments, ranging from dental diseases to nondental diseases. The present review has summarized dental MSC sources, multi-lineage differentiation capacities, immunomodulatory features, its potential in the treatment of diseases, and its application in both preclinical studies and clinical trials. The regenerative therapeutic strategies in dental medicine have also been discussed.

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Dental and Nondental Stem Cell Based Regeneration of the Craniofacial Region: A Tissue Based Approach

Cited by 18 publications

References 116 publications

Retinoic Acid Signal Negatively Regulates Osteo/Odontogenic Differentiation of Dental Pulp Stem Cells

Retinoic Acid Signal Negatively Regulates Osteo/Odontogenic Differentiation of Dental Pulp Stem Cells

Evaluation of Poly(Lactic-co-glycolic) Acid Alone or in Combination with Hydroxyapatite on Human-Periosteal Cells Bone Differentiation and in Sinus Lift Treatment

Dental Tissue-Derived Human Mesenchymal Stem Cells and Their Potential in Therapeutic Application

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