Dental-derived stem cells in tissue engineering: the role of biomaterials and host response

Yuan, Weihao; Ferreira, Luiza de Almeida Queiroz; Yu, Bo; Ansari, Sahar; Moshaverinia, Alireza

doi:10.1093/rb/rbad100

Cited by 4 publications

(2 citation statements)

References 117 publications

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“…Another attractive cell source for regenerative medicine and tissue engineering is dental-derived stem cells (DSCs) due to their accessibility compared to that of bone marrow or adipose-derived MSCs, low immunogenicity, robust growth capacity, and multilineage potential [ 30 ]. This category includes dental pulp-derived stem cells (DPSCs), dental follicle-derived MSCs (DFSCs), stem cells derived from human exfoliated deciduous teeth (SHED), and periapical cysts MSCs (PCy-MSCs) [ 31 ].…”

Section: Bone Tissue Engineering: Combining Stem Cells Scaffolds the ...mentioning

confidence: 99%

Towards Stem Cell Therapy for Critical-Sized Segmental Bone Defects: Current Trends and Challenges on the Path to Clinical Translation

Quek,

Vizetto-Duarte,

Teoh

et al. 2024

JFB

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The management and reconstruction of critical-sized segmental bone defects remain a major clinical challenge for orthopaedic clinicians and surgeons. In particular, regenerative medicine approaches that involve incorporating stem cells within tissue engineering scaffolds have great promise for fracture management. This narrative review focuses on the primary components of bone tissue engineering—stem cells, scaffolds, the microenvironment, and vascularisation—addressing current advances and translational and regulatory challenges in the current landscape of stem cell therapy for critical-sized bone defects. To comprehensively explore this research area and offer insights for future treatment options in orthopaedic surgery, we have examined the latest developments and advancements in bone tissue engineering, focusing on those of clinical relevance in recent years. Finally, we present a forward-looking perspective on using stem cells in bone tissue engineering for critical-sized segmental bone defects.

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Section: Bone Tissue Engineering: Combining Stem Cells Scaffolds the ...mentioning

confidence: 99%

Towards Stem Cell Therapy for Critical-Sized Segmental Bone Defects: Current Trends and Challenges on the Path to Clinical Translation

Quek,

Vizetto-Duarte,

Teoh

et al. 2024

JFB

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“…While BMSCs can be isolated from the iliac crest, they are also obtainable from orofacial bones [80,81]. Human BMSCs, specifically from the maxilla and mandible, can be isolated from bone marrow aspirated during dental procedures such as dental implantation, third molar extraction, orthodontic osteotomy, or cyst extirpation [82][83][84][85]. The autotransplantation of MSCs derived from bone marrow has demonstrated the ability to stimulate regeneration up to 20% in both cementum and alveolar bone.…”

Section: Bone-marrow-derived Mscs (Bmscs) For Periodontal Regenerationmentioning

confidence: 99%

Insights and Advancements in Periodontal Tissue Engineering and Bone Regeneration

Angjelova,

Jovanova,

Polizzi

et al. 2024

Medicina

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The regeneration of periodontal bone defects continues to be an essential therapeutic concern in dental biomaterials. Numerous biomaterials have been utilized in this sector so far. However, the immune response and vascularity in defect regions may be disregarded when evaluating the effectiveness of biomaterials for bone repair. Among several regenerative treatments, the most recent technique of in situ tissue engineering stands out for its ability to replicate endogenous restorative processes by combining scaffold with particular growth factors. Regenerative medicine solutions that combine biomaterials/scaffolds, cells, and bioactive substances have attracted significant interest, particularly for bone repair and regeneration. Dental stem cells (DSCs) share the same progenitor and immunomodulatory properties as other types of MSCs, and because they are easily isolable, they are regarded as desirable therapeutic agents in regenerative dentistry. Recent research has demonstrated that DSCs sown on newly designed synthetic bio-material scaffolds preserve their proliferative capacity while exhibiting increased differentiation and immuno-suppressive capabilities. As researchers discovered how short peptide sequences modify the adhesion and proliferative capacities of scaffolds by activating or inhibiting conventional osteogenic pathways, the scaffolds became more effective at priming MSCs. In this review, the many components of tissue engineering applied to bone engineering will be examined, and the impact of biomaterials on periodontal regeneration and bone cellular biology/molecular genetics will be addressed and updated.

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Translational implications of a novel combination of iPRF and collagen scaffold for proliferation of gingival mesenchymal stem cells

Ghadge,

Shetty,

Kharat

et al. 2024

Sci Rep

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“Tissue engineering,” is a concept that involves the use of scaffolds, cells, and growth factors/signaling molecules in the field of regenerative medicine. The present study was carried out to evaluate the attachment and depth of penetration of the Gingival Mesenchymal Stem Cells ( GMSCs) onto the collagen scaffold preconditioned with Fetal Bovine Serum (FBS), Injectable Platelet Rich Fibrin (i-PRF) and a combination of FBS with i-PRF using histological analysis and environmental scanning electron microscopy (ESEM) respectively. In the present study, commercially available collagen membranes were used as scaffolds and divided into 3 groups where Group I was preconditioned with FBS, Group II was preconditioned with i-PRF, and Group III with a combination of FBS and i-PRF. Scaffolds were then seeded with GMSCs and incubated in a CO 2 incubator at 37 0 C for 7 days. Both histological and ESEM analysis showed proliferation, attachment, and depth of penetration along with the combination of cell morphological changes in all the three groups. In group I cells showed mild depth of penetration along with a round morphological appearance. In group II the cells showed moderate depth of penetration and rounded morphology. In group III maximum depth of penetration was seen along with the round and spheroidal morphology of cells. The combination of the growth media showed the best effect on cell attachment, depth of penetration, and cell morphology. This is the first report demonstrating the effect of the combination of collagen and i-PRF supports the proliferation of GMSCs. Since FBS alone and i-PRF alone exhibited similar cell proliferation it is recommended that i-PRF could be used in clinical scenarios making it xenofree.

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Dental-derived stem cells in tissue engineering: the role of biomaterials and host response

Cited by 4 publications

References 117 publications

Towards Stem Cell Therapy for Critical-Sized Segmental Bone Defects: Current Trends and Challenges on the Path to Clinical Translation

Towards Stem Cell Therapy for Critical-Sized Segmental Bone Defects: Current Trends and Challenges on the Path to Clinical Translation

Insights and Advancements in Periodontal Tissue Engineering and Bone Regeneration

Translational implications of a novel combination of iPRF and collagen scaffold for proliferation of gingival mesenchymal stem cells

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