2012
DOI: 10.1002/jbm.b.32693
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Dental implants stimulate expression of Interleukin‐8 and its receptor in human blood—An in vitro approach

Abstract: Interleukin (IL)‐8 secreted from osteoblasts and peripheral blood monocytes increases in patients with aseptic hip‐implant loss and in patients with mucositis after dental implant insertion. We explored in vitro the possibility of an IL‐8‐mediated inflammatory response as a consequence of contact between different dental implant surfaces and human blood. Titanium and zirconia implants were incubated in human blood. Nonstimulated blood served as negative, while blood stimulated with bacterial lipopolysaccharide… Show more

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Cited by 19 publications
(12 citation statements)
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“…cDNA synthesis and. qPCR was performed as previously described (26). e6/e7 primers are described elsewhere (27).…”
Section: Methodsmentioning
confidence: 99%
“…cDNA synthesis and. qPCR was performed as previously described (26). e6/e7 primers are described elsewhere (27).…”
Section: Methodsmentioning
confidence: 99%
“…RNA (200 ng) was transcribed into cDNA (cDNA synthesis kit AmpTec, Hamburg, Germany) according to the manufacturer's protocol. qPCR and primer design was performed as described previously, using the following primers at 60°C annealing temperature: SLPI , Forward: 3′‐AATgCCTggATCCTgTTgAC‐5′; SLPI , Reverse: 3′‐AAAggACCTggACCACACAg‐5′; annexin 2 , Forward: 3′‐AACCgACgAggACTCTCTCA‐5′; annexin 2 , Reverse: 3′‐CgCTgATCCACTTgggAACAT‐5′; AhR , Forward: 3′‐gTTggACgTCagCAAgTTCA‐5′; AhR , Reverse: 3′‐TggTgCCCAgAATAATgTgA‐5′; α7AChR , Forward: 3′‐ggACAAggTgCgCCCggCCTg‐5′; α7AChR , Reverse: 3′‐CACACAggCggCgAACTTCCACT‐5′. Primers for the house keeping gene 18S rRNA were purchased from Promolgene (Berlin, Germany) and used according to the manufacturer's protocol.…”
Section: Methodsmentioning
confidence: 99%
“…These lesions are associated with colonization of peri‐implant tissues by bacterial biofilms that elicit an inflammatory response, which is regulated by cytokines. Proinflammatory cytokines released by monocytes and macrophages, in response to bacterial products such as lipopolysaccharides, are associated with degradation of peri‐implant tissues 3,6‐8 . Some of these cytokines and their signaling receptors form complex networks, which modify the inflammatory response 9 …”
mentioning
confidence: 99%
“…Proinflammatory cytokines released by monocytes and macrophages, in response to bacterial products such as lipopolysaccharides, are associated with degradation of peri-implant tissues. 3,[6][7][8] Some of these cytokines and their signaling receptors form complex networks, which modify the inflammatory response. 9 The clinical stability of peri-implant tissues and the maintenance of bone around implants depend on homeostatic systems that include reactive and suppressor immune cells and their associated regulatory cytokines and mediators.…”
mentioning
confidence: 99%