Dental mesenchymal stem cells and neuro-regeneration: a focus on spinal cord injury

Bonaventura, Gabriele; Incontro, Salvatore; Iemmolo, Rosario; Cognata, Valentina La; Barbagallo, Ignazio; Costanzo, Erminio; Barcellona, Maria Luisa; Pellitteri, Rosalia; Cavallaro, Sebastiano

doi:10.1007/s00441-019-03109-4

Cited by 36 publications

(31 citation statements)

References 75 publications

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“…This lipid screening was done by preparing the blank SLNs using different lipid matrices such as stearic acid, Compritol 888 ATO, and Precirol 5 ATO; the lipid concentrations were chosen based on the amount we used to prepare pSLNs. Subsequently, we investigated the effect of these nanocarriers on the cell viability of adult primary stem cells [ 34 , 35 ]. We chose Dental Pulp Stem Cells (DPSCs) transdifferentiated in Neural Stem Cells (NSCs) because they possess protective effects against models of neurodegenerative diseases, including Alzheimer’s disease [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

Curcumin Containing PEGylated Solid Lipid Nanoparticles for Systemic Administration: A Preliminary Study

et al. 2020

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Curcumin (CUR) has a wide range of pharmacological properties, including anti-inflammatory and antioxidant activities, and it can be considered a good candidate for the potential treatment of central nervous system (CNS) pathologies, although its use in clinical practice is compromised due to its high lipophilicity. Solid lipid nanoparticles (SLNs) are well-known nanocarriers representing a consolidated approach for the delivery of lipophilic compounds, but their systemic use is limited due their short half-life. The formulation of stealth SLNs (pSLNs) could be a valid strategy to overcome this limit. Curcumin-loaded-pSLNs were prepared by the solvent evaporation method. Formulation was characterized for their mean size, zeta potential, size distribution, and morphology. Drug antioxidant activity was evaluated by Oxygen Radical Absorbance Capacity (ORAC) assay. Finally, the obtained formulations were analyzed in terms of long-term stability. Curcumin-loaded-pSLNs showed good technological parameters with a mean particle size below 200 nm, as confirmed by TEM images, and a zeta potential value around −30 mV, predicting good long-term stability. Differential Scanning Calorimetry (DSC) analysis confirmed that PEG micelles interacted with the SLN surface; this suggests the location of the PEG on the pSLN surface. Therefore, these preliminary studies suggest that the produced formulation could be regarded as a promising carrier for the systemic administration.

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Section: Resultsmentioning

confidence: 99%

Curcumin Containing PEGylated Solid Lipid Nanoparticles for Systemic Administration: A Preliminary Study

et al. 2020

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“…Pointing to the importance of ECM in maintaining homeostasis for proliferation and differentiation of DSCs, several studies have indicated that reconstruction of the appropriate microenvironment and boosting its interaction with stem cells are essential steps to successful cell therapy[ 50 ]. The application of DSCs in stem cell therapeutic approaches is attractive due to their simple isolation and efficient administration[ 51 ]. The multi-lineage capacity of DSCs differentiation to various tissues and organs suggests their greater ability than other adult stem cell populations for the treatment of different diseases[ 52 ].…”

Section: Introductionmentioning

confidence: 99%

Dental stem cells: The role of biomaterials and scaffolds in developing novel therapeutic strategies

Granz

Gorji

2020

WJSC

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Dental stem cells (DSCs) are self-renewable cells that can be obtained easily from dental tissues, and are a desirable source of autologous stem cells. The use of DSCs for stem cell transplantation therapeutic approaches is attractive due to their simple isolation, high plasticity, immunomodulatory properties, and multipotential abilities. Using appropriate scaffolds loaded with favorable biomolecules, such as growth factors, and cytokines, can improve the proliferation, differentiation, migration, and functional capacity of DSCs and can optimize the cellular morphology to build tissue constructs for specific purposes. An enormous variety of scaffolds have been used for tissue engineering with DSCs. Of these, the scaffolds that particularly mimic tissue-specific micromilieu and loaded with biomolecules favorably regulate angiogenesis, cell-matrix interactions, degradation of extracellular matrix, organized matrix formation, and the mineralization abilities of DSCs in both in vitro and in vivo conditions. DSCs represent a promising cell source for tissue engineering, especially for tooth, bone, and neural tissue restoration. The purpose of the present review is to summarize the current developments in the major scaffolding approaches as crucial guidelines for tissue engineering using DSCs and compare their effects in tissue and organ regeneration.

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“…In Vitro Study on AST-SSLN 2.9.1. Cell Cultures: Treatment and MTT Assay Human dental pulp stem cells (hDPSCs) were isolated from teeth of anonymous donors/patients, according to the ethical Committee of Azienda Ospedaliero-Universitaria "Policlinico-Vittorio Emanuele" (Catania, Italy) [36]. Briefly, freshly extracted teeth were opened and the dental pulp tissue was taken, fragmented, digested with 3 mg/mL collagenase type I for 1 h at 37 • C and centrifuged at 200× g for 15 min.…”

Section: 9mentioning

confidence: 99%

“…The choice of the most suitable lipid matrix to formulate AST-SSLN was first determined using the MTT assay, since this test proved useful for revealing the potential cytotoxic effects of excipients on adult primary stem cells (primary adult stem cell human dental pulp, DPSCs) [36,43]. DSPCs isolated from human dental pulp and differentiated into neurons, derive from the embryonic neural crest and represent a useful source of primary cells for modeling neurological disorders at the molecular level [44,45].…”

Section: Screening Of Lipid Matrixmentioning

confidence: 99%

Astaxanthin-Loaded Stealth Lipid Nanoparticles (AST-SSLN) as Potential Carriers for the Treatment of Alzheimer’s Disease: Formulation Development and Optimization

et al. 2021

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Alzheimer’s disease (AD) is a neurodegenerative disorder associated with marked oxidative stress at the level of the brain. Recent studies indicate that increasing the antioxidant capacity could represent a very promising therapeutic strategy for AD treatment. Astaxanthin (AST), a powerful natural antioxidant, could be a good candidate for AD treatment, although its use in clinical practice is compromised by its high instability. In order to overcome this limit, our attention focused on the development of innovative AST-loaded stealth lipid nanoparticles (AST-SSLNs) able to improve AST bioavailability in the brain. AST-SSLNs prepared by solvent-diffusion technique showed technological parameters suitable for parenteral administration (<200 nm). Formulated nanosystems were characterized by calorimetric studies, while their toxicological profile was evaluated by the MTT assay on the stem cell line OECs (Olfactory Ensheathing Cells). Furthemore, the protective effect of the nanocarriers was assessed by a long-term stability study and a UV stability assay confirming that the lipid shell of the nanocarriers was able to preserve AST concentration in the formulation. SSLNs were also capable of preserving AST’s antioxidant capacity as demonstrated in the oxygen radical absorbance capacity (ORAC) assay. In conclusion, these preliminary studies outline that SSLNs could be regarded as promising carriers for systemic administration of compounds such as AST aimed at AD treatment.

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Dental mesenchymal stem cells and neuro-regeneration: a focus on spinal cord injury

Cited by 36 publications

References 75 publications

Curcumin Containing PEGylated Solid Lipid Nanoparticles for Systemic Administration: A Preliminary Study

Curcumin Containing PEGylated Solid Lipid Nanoparticles for Systemic Administration: A Preliminary Study

Dental stem cells: The role of biomaterials and scaffolds in developing novel therapeutic strategies

Astaxanthin-Loaded Stealth Lipid Nanoparticles (AST-SSLN) as Potential Carriers for the Treatment of Alzheimer’s Disease: Formulation Development and Optimization

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