Dental Pulp Mesenchymal Stem Cells as a Treatment for Periodontal Disease in Older Adults

Hernández-Monjaraz, Beatriz; Santiago‐Osorio, Edelmiro; Ledesma‐Martínez, Edgar; Aguíñiga-Sánchez, Itzen; Sosa-Hernández, Norma Angélica; Mendoza-Núñez, Vı́ctor Manuel

doi:10.1155/2020/8890873

Cited by 27 publications

(11 citation statements)

References 90 publications

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“…36 These and other experimental data present strong evidence that DPSCs have the potential to induce the formation of human dental tissues in vivo; however, clinical data were available for only a few human cases. 37 Thus, the present case report examined whether the transplantation of human DPSCs contributed to periodontal repair in a patient with a periodontal disease-induced infrabony defect.…”

Section: Discussionmentioning

confidence: 96%

Retrieval of a periodontally compromised tooth by allogeneic grafting of mesenchymal stem cells from dental pulp: A case report

et al. 2018

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ObjectiveTo report a case of successful allogeneic grafting of mesenchymal dental pulp stem cells (DPSCs) as preliminary findings in a patient with periodontal disease enrolled into clinical trial ISRCTN12831118.MethodsMesenchymal stem cells from the dental pulp of a deciduous tooth from a 7-year-old donor were separated from the pulp chamber and processed via enzymatic digestion and centrifugation. DPSCs were passaged and cultured on a 35 × 13 mm culture dish in minimum essential medium-alpha, without supplementation. After reaching 80% confluency, 5 x 106 allogeneic DPSCs in 250 µl phosphate buffered saline were seeded onto a dry scaffold of lyophilized collagen-polyvinylpyrrolidone sponge placed in the left lower premolar area of a 61-year-old patient with periodontal disease. Surgical access to the lower premolar area was achieved using the flap technique.ResultsAt 3 and 6 months following allogeneic graft, the patient showed no sign of rejection and exhibited decreases in tooth mobility, periodontal pocket depth and bone defect area. Bone mineral density had increased at the graft site.ConclusionsRegenerative periodontal therapy using DPSCs of allogeneic origin may be a promising treatment for periodontal disease-induced bone defects.

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Section: Discussionmentioning

confidence: 96%

Retrieval of a periodontally compromised tooth by allogeneic grafting of mesenchymal stem cells from dental pulp: A case report

et al. 2018

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“…Resident dental MSCs are involved in both inflammatory response and dental tissue repair after trauma. The clinical protocols for applying dental MSCs for the treatment of periodontal and endodontic defects are currently developing [151][152][153]. Besides, due to their accessibility and functional properties, dental MSCs have an enormous potential for application beyond the dental field [23].…”

Section: Concluding Remarks and Future Perspectivementioning

confidence: 99%

Toll-Like Receptors and Dental Mesenchymal Stromal Cells

Andrukhov

2021

Front. Oral. Health

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Dental mesenchymal stromal cells (MSCs) are a promising tool for clinical application in and beyond dentistry. These cells possess multilineage differentiation potential and immunomodulatory properties. Due to their localization in the oral cavity, these cells could sometimes be exposed to different bacteria and viruses. Dental MSCs express various Toll-like receptors (TLRs), and therefore, they can recognize different microorganisms. The engagement of TLRs in dental MSCs by various ligands might change their properties and function. The differentiation capacity of dental MSCs might be either inhibited or enhanced by TLRs ligands depending on their nature and concentrations. Activation of TLR signaling in dental MSCs induces the production of proinflammatory mediators. Additionally, TLR ligands alter the immunomodulatory ability of dental MSCs, but this aspect is still poorly explored. Understanding the role of TLR signaling in dental MSCs physiology is essential to assess their role in oral homeostasis, inflammatory diseases, and tissue regeneration.

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“…The odontogenic abilities of hDPSCs are crucial for this physiological process [ 30 ]. Recently, clinical trials for hDPSC-mediated therapies have rapidly developed, and there is a high potential for using hDPSCs as a novel therapy for many diseases, such as periodontal disease [ 31 ], neurological diseases [ 32 ], and stroke [ 33 , 34 ]. However, there is no research available regarding the effect of EDA and its mutant on the odontogenic differentiation abilities of hDPSCs.…”

Section: Discussionmentioning

confidence: 99%

Functional Analysis of Ectodysplasin-A Mutations in X-Linked Nonsyndromic Hypodontia and Possible Involvement of X-Chromosome Inactivation

Pan

Lü

Peng

et al. 2021

Stem Cells International

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Background. Mutations of the Ectodysplasin-A (EDA) gene are generally associated with syndrome hypohidrotic ectodermal dysplasia or nonsyndromic tooth agenesis. The influence of EDA mutations on dentinogenesis and odontoblast differentiation has not been reported. The aim of this study was to identify genetic clues for the causes of familial nonsyndromic oligodontia and explore the underlying mechanisms involved, while focusing on the role of human dental pulp stem cells (hDPSCs). Materials and Methods. Candidate gene sequences were obtained by PCR amplification and Sanger sequencing. Functional analysis was conducted, and the pathogenesis associated with EDA mutations in hDPSCs was investigated to explore the impact of the identified mutation on the phenotype. Capillary electrophoresis (CE) was used to detect X-chromosome inactivation (XCI) in the blood of female carriers. Results. In this study, we identified an EDA mutation in a Chinese family: the missense mutation c.1013C>T (Thr338Met). Transfection of hDPSCs with a mutant EDA lentivirus decreased the expression of EDA and dentin sialophosphoprotein (DSPP) compared with transfection of control EDA lentivirus. Mechanistically, mutant EDA inhibited the activation of the NF-κB pathway. The CE results showed that symptomatic female carriers had a skewed XCI with a preferential inactivation of the X chromosome that carried the normal allele. Conclusions. In summary, we demonstrated that EDA mutations result in nonsyndromic tooth agenesis in heterozygous females and that, mechanistically, EDA regulates odontogenesis through the NF-κB signalling pathway in hDPSCs. Due to the large heterogeneity of tooth agenesis, this study provided a genetic basis for individuals who exhibit similar clinical phenotypes.

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Dental Pulp Mesenchymal Stem Cells as a Treatment for Periodontal Disease in Older Adults

Cited by 27 publications

References 90 publications

Retrieval of a periodontally compromised tooth by allogeneic grafting of mesenchymal stem cells from dental pulp: A case report

Retrieval of a periodontally compromised tooth by allogeneic grafting of mesenchymal stem cells from dental pulp: A case report

Toll-Like Receptors and Dental Mesenchymal Stromal Cells

Functional Analysis of Ectodysplasin-A Mutations in X-Linked Nonsyndromic Hypodontia and Possible Involvement of X-Chromosome Inactivation

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