Dental Pulp Tissue Engineering with Stem Cells from Exfoliated Deciduous Teeth

Cordeiro, Mabel Mariela Rodríguez; Dong, Zhihong; Kaneko, Tomoyuki; Zhang, Zhaocheng; Miyazawa, Marta; Shi, Songtao; Smith, Anthony J.; Nör, Jacques E.

doi:10.1016/j.joen.2008.04.009

Cited by 574 publications

(509 citation statements)

References 33 publications

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“…The teeth were then cut transversally in the cervical region with a diamond-edged blade at low speed under cooling with sterile PBS to obtain slices 1-mm thick and a wide pulp chamber. 20,21,22 The pulp tissue was carefully pulled away with tissue forceps, without touching the predentin layer, which could be visually identified by its whitish color in comparison with the yellow dentin.…”

Section: Methodology Tooth Slice Preparationmentioning

confidence: 99%

Effect of EDTA on TGF-β1 released from the dentin matrix and its influence on dental pulp stem cell migration

et al. 2016

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Bioactive molecules stored in dentin, such as transforming growth factor beta1 (TGF-b1), may be involved in the signaling events related to dental tissue repair. The authors conducted an in vitro evaluation of the amount of TGF-b1 released from dentin slices after treatment with 10% ethylenediaminetetraacetic acid (EDTA), 2.5% sodium hypochlorite (NaOCl) or phosphate-buffered saline (PBS), and the effect of this growth factor on stem cell migration from human exfoliated deciduous teeth (SHED). Sixty 1-mm-thick tooth slices were prepared with or without the predentin layer, and treated with either 10% EDTA for 1 minute, 2.5% NaOCl for 5 days or kept in PBS. Tooth slice conditioned media were prepared and used for TGF-b1 ELISA and migration assays. Culture medium with different concentrations of recombinant human TGF-b1 (0.5, 1.0, 5.0 or 10.0 ng/mL) was also tested by migration assay. The data were evaluated by ANOVA and Tukey's test. Optical density values corresponding to media conditioned by tooth slices either containing or not containing the predentin layer and treated with 10% EDTA were statistically greater than the other groups and close to 1 ng/mL. Increased rates of migration toward media conditioned by tooth slices containing the predentin layer and treated with PBS, 10% EDTA or 2.5% NaOCl were observed. Recombinant human TGF-b1 also stimulated migration of SHED, irrespective of the concentration used. EDTA may be considered an effective extractant of TGF-b1 from the dentin matrix. However, it does not impact SHED migration, suggesting that other components may account for the cell migration.

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Section: Methodology Tooth Slice Preparationmentioning

confidence: 99%

Effect of EDTA on TGF-β1 released from the dentin matrix and its influence on dental pulp stem cell migration

et al. 2016

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“…Human pulp-derived stem cells (hDPSCs) possess high proliferation potential with multi-lineage differentiation capacity compare to the ordinary source of adult stem cells [1][2][3][4][5][6][7][8] …”

Section: Introductionmentioning

confidence: 99%

“…Characteristically, similar to the mesenchymal stem cells, dental pulp stem cells are adherent clonogenic cells which have multiple differentiation capacity into mesenchyme and/or non-mesenchyme lineages, both in vitro and in vivo. [1][2][3][4][5][6][7][8]17,18 DPSCs are identified by their negative expression of hematopoietic antigens (e.g., CD45, CD34, CD14), and positive expression of CD90, CD29, CD73, CD105, CD44 and STRO-1. 19,20 Easy obtained potential with minimum pain & morbidity make human DPSCs as a valuable source of MSCs compared to the ordinary sources, such as bone marrow mesenchymal stem cells 21 .…”

Section: Introductionmentioning

confidence: 99%

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Karamzadeh

Eslaminejad

Aflatoonian

2012

JoVE

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“…Consequently, the ingrowing tissue had to be triggered either by the fibrin gel itself or by tissue factors released from the dentinal wall. Both cell-based and cell-homing approaches showed various aspects necessary for pulp regeneration (14)(15)(16). Some of these studies already focused on the use of irrigating solutions (10,17) and the type of scaffold (18)(19)(20).…”

Section: Discussionmentioning

confidence: 99%

Fibrin Gel Improves Tissue Ingrowth and Cell Differentiation in Human Immature Premolars Implanted in Rats

Ruangsawasdi

Zehnder

Weber

2014

Journal of Endodontics

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INTRODUCTION In pulpless immature human premolars implanted in rodents, this study investigated whether fibrin gel offered advantages over leaving the root canal empty regarding soft tissue ingrowth and cell differentiation. METHODS Root canals of extracted human immature premolars (n = 12) were accessed and then irrigated with 5% sodium hypochlorite followed by 17% ethylenediaminetetraacetic acid. Root canals were then either left empty or filled with a fibrin gel (n = 6 each) before being placed subcutaneously on top of the calvarial bone of rats (1 tooth per rat) for 12 weeks. After sacrifice, teeth were histologically assessed. Tissue ingrowth was quantified and compared between groups using the Mann-Whitney U test (P < .05). Cells adhering to the pulp canal wall were immunohistochemically screened for the presence of bone sialoprotein (BSP) and dentin sialoprotein (DSP). RESULTS More tissue grew into the pulp space when teeth were filled with fibrin gel (P < .05). The presence of fibrin gel affected not only the extent of tissue ingrowth but also tissue morphology and differentiation of cells contacting the dentinal wall. In the fibrin gel group, newly formed tissue was similar to normal pulp, constituted of inner pulp, cell-rich zone, cell-free zone, and an apparent odontoblast layer, which stained positive for BSP and DSP. Newly formed blood vessels were also more abundant compared with the initially empty root canals. CONCLUSIONS Under the conditions of this study, fibrin gel improved cell infiltration and cell-dentin interaction. Both are necessary for pulp tissue regeneration.

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Dental Pulp Tissue Engineering with Stem Cells from Exfoliated Deciduous Teeth

Cited by 574 publications

References 33 publications

Effect of EDTA on TGF-β1 released from the dentin matrix and its influence on dental pulp stem cell migration

Effect of EDTA on TGF-β1 released from the dentin matrix and its influence on dental pulp stem cell migration

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Fibrin Gel Improves Tissue Ingrowth and Cell Differentiation in Human Immature Premolars Implanted in Rats

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