Dental Stem Cells Harvested from Third Molars Combined with Bioactive Glass Can Induce Signs of Bone Formation In Vitro

Raspini, Gregorio; Wolff, Jan; Helminen, Mika; Raspini, Giovacchino; Raspini, Mario; Sándor, George K.B.

doi:10.5037/jomr.2018.9102

Cited by 5 publications

(3 citation statements)

References 34 publications

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“…46 hDPSC have relevant advantages including its high availability for autologous transplantation, which minimizes immunological rejection and ethical concerns. 47 hDPSC are multipotent cell populations largely present in the pulp chamber. hDPSC display mesenchymal-like characteristics, including self-renewal immunomodulatory properties, multi-lineage differentiation potential, and migration to the site of injury.…”

Section: The Emerging Role Of Dental Pulp-derived Stem Cellsmentioning

confidence: 99%

Trends in Salivary Gland Tissue Engineering: From Stem Cells to Secretome and Organoid Bioprinting

Chansaenroj

Yodmuang

Ferreira

2021

Tissue Engineering Part B: Reviews

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Xerostomia or dry mouth are commonly diagnosed in head and neck cancer patients due to salivary gland (SG) epithelial injury after radiotherapy. Regenerative medicine has fetched the opportunity to replace or regenerate the SG epithelia and restore its secretory function. Early adult stem cell transplantation strategies in rodents have recently shown to improve clinical outcomes in radiotherapy-induced xerostomia in Phase 1/2 human trials. Mesenchymal stem cells from adipose tissue are the most promising, although the ones from the labial mucosa, bone marrow, or dental pulp have an attractive therapeutic value after successful findings in ex vivo and in vivo mouse models of SG injury. Emerging approaches using cell-free therapy with cell ''extracts'', ''soups'' or secretome components also exhibit favorable outcomes in the same rodent models. When compared to cell-based approaches, extracellular vesicles (EV) from the secretome (i.e., exosomes) can be easily extracted, quantified, and are more stable for long-term storage and use in SG tissue engineering. Additive manufacturing and threedimensional bioprinting or bioassembly have an important role on generating spheroids or organoids for cell transplantation to ameliorate SG injury. Moreover, organoids can secrete EV, which may have a therapeutic potential worth to explore in future studies. In this review, we will describe the technological advancements and challenges of these different cell-based and cell-free strategies in SG tissue engineering and regeneration.

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Section: The Emerging Role Of Dental Pulp-derived Stem Cellsmentioning

confidence: 99%

Trends in Salivary Gland Tissue Engineering: From Stem Cells to Secretome and Organoid Bioprinting

Chansaenroj

Yodmuang

Ferreira

2021

Tissue Engineering Part B: Reviews

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“…Stem cells have been successfully isolated from human teeth and were studied to test their ability to regenerate dental structures and periodontal tissues. MSCs were reported to be successfully isolated from dental tissues like dental pulp of permanent and deciduous teeth, periodontal ligament, apical papilla and dental follicle (42)(43)(44). These cells were described as an excellent cell source owing to their ease of accessibility, their ability to differentiate into osteoblasts and odontoblasts and lack of ethical controversies (45).…”

Section: Stem Cells In Dentistrymentioning

confidence: 99%

Current state of stem cell-based therapies: an overview

Aly

2020

Stem Cell Investig

127

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Recent research reporting successful translation of stem cell therapies to patients have enriched the hope that such regenerative strategies may one day become a treatment for a wide range of vexing diseases. In fact, the past few years witnessed, a rather exponential advancement in clinical trials revolving around stem cell-based therapies. Some of these trials resulted in remarkable impact on various diseases.In this review, the advances and challenges for the development of stem-cell-based therapies are described, with focus on the use of stem cells in dentistry in addition to the advances reached in regenerative treatment modalities in several diseases. The limitations of these treatments and ongoing challenges in the field are also discussed while shedding light on the ethical and regulatory challenges in translating autologous stem cellbased interventions, into safe and effective therapies.

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“…Dental pulp stem cells are also of interest due to their ease of access, low donor site morbidity, and ability to differentiate into fibroblasts, nerve cells, endothelial cells, and odontoblasts in order to facilitate creation of new connective tissue [89] . Raspini et al [90] showed that dental pulp stem cells combined with bioactive glass scaffold that was treated with osteogenic medium in vitro showed good biocompatibility and osteogenic induction, making it a promising combination for hard tissue regeneration in the cranio-maxillofacial skeleton. However, the comparative efficacy of these cells between laboratory study and patient intervention remains to be seen [91] .…”

Section: Stem Cells and Growth Factorsmentioning

confidence: 99%

Tissue engineering in mandibular reconstruction: osteogenesis-inducing scaffolds

Nelms¹,

Palmer²

2019

PAR

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Currently, the gold standard for aesthetic and functional reconstruction of critical mandibular defects is an autologous fibular flap; however, this carries risk of donor site morbidity, and is not a promising option in patients with depleted donor sites due to previous surgeries. Tissue engineering presents a potential solution in the design of a biomimetic scaffold that must be osteoconductive, osteoinductive, and support osseointegration. These osteogenesis-inducing scaffolds are most successful when they mimic and interact with the surrounding native macroand micro-environment of the mandible. This is accomplished via the regeneration triad: (1) a biomimetic, bioactive osteointegrative scaffold, most likely a resorbable composite of collagen or a synthetic polymer with collagen-like properties combined with beta-tri calcium phosphate that is 3D printed according to defect morphology; (2) growth factor, most frequently bone morphogenic protein 2 (BMP-2); and (3) stem cells, most commonly bone marrow mesenchymal stem cells. Novel techniques for scaffold modification include the use of nano-hydroxyapatite, or combining a vector with a biomaterial to create a gene activated matrix that produces proteins of interest (typically BMP-2) to support osteogenesis. Here, we review the current literature in tissue engineering in order to discuss the success of varying use and combinations of scaffolding materials (i.e., ceramics, biological polymers, and synthetic polymers) with stem cells and growth factors, and will examine their success in vitro and in vivo to induce and guide osteogenesis in mandibular defects.

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Dental Stem Cells Harvested from Third Molars Combined with Bioactive Glass Can Induce Signs of Bone Formation In Vitro

Cited by 5 publications

References 34 publications

Trends in Salivary Gland Tissue Engineering: From Stem Cells to Secretome and Organoid Bioprinting

Trends in Salivary Gland Tissue Engineering: From Stem Cells to Secretome and Organoid Bioprinting

Current state of stem cell-based therapies: an overview

Tissue engineering in mandibular reconstruction: osteogenesis-inducing scaffolds

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