Dental Tissue-Derived Mesenchymal Stem Cells: Applications in Tissue Engineering

Dave, Jay R.; Tomar, Geetanjali B.

doi:10.1615/critrevbiomedeng.2018027342

Cited by 48 publications

(40 citation statements)

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“…[ 19 ] These stem cells primarily include the tooth-related stem cells DPSCs, SHEDs, GMSCs, PDLSCs, dental follicle progenitor cells (DFPCs), stem cells from the apical papilla (SCAPs), tooth germ stem cells (TGSCs), ABMSCs, and the saliva gland-related stem cells submandibular gland epithelial stem cells (SMGepiSCs) [ 28 ]. Ease of tissue harvest, low population-doubling time, plasticity, multipotential capabilities, and immunomodulatory properties make these cells suitable candidates for various therapeutic strategies [ 20 , 29 ]. In some respects, these oral tissue stem cells showed a variety of good characteristics compared with BMSCs and ADSCs as we have mentioned above, which may related to the unique tissue origin of the oral cavity and the complexity of the oral environment.…”

Section: Oral Tissue Stem Cells and Exosomesmentioning

confidence: 99%

“…Different stem cell populations with different remarkable properties were used in different areas. Oral tissue-derived stem cells recently gained more attention because these cells are rich in source and retrieved via noninvasive procedures [ 19 , 20 ]. Many kinds of stem cells were isolated from oral tissue, such as dental pulp stem cells (DPSCs), stem cells from apical papilla (SCAPs), and stem cells from human exfoliated deciduous teeth (SHEDs).…”

Section: Introductionmentioning

confidence: 99%

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Exosomes from oral tissue stem cells: biological effects and applications

et al. 2020

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As natural nanoparticles, exosomes are a type of extracellular vesicles that are enclosed by a lipid bilayer and contain various cargos, including miRNA, mRNA, DNA and proteins. Exosomes have rapidly gained attention as a highly promising cell-free therapy. Because the cargo of exosomes changes with the changes in parent cells and status, exosomes from different types of cells may exhibit different biological effects. Considering the particularity of oral tissue stem cells, their exosomes were isolated and used to examine their related biological functions and the possibility of replacing stem cells. A variety of exosomes of oral tissue stem cells were studied, and the results revealed many special biological characteristics of these exosomes and their parent cells, especially immunomodulation, osteogenesis, odontogenesis, neuroprotection, nerve regeneration, wound healing, skin regeneration and vascularization. The oral tissue stem cell exosomes may be loaded with drugs or genes and act as tools for tumor treatment. The relevant results showed that exosomes from oral tissue stem cells were potent therapeutic tools. The present review focuses on the biological function and application of oral tissue stem cell-derived exosomes.

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Section: Oral Tissue Stem Cells and Exosomesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Exosomes from oral tissue stem cells: biological effects and applications

et al. 2020

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“…The ease of tissue collection, high initial cell yield, low population doubling time, plasticity, multipotential capacities, and immunomodulatory properties make MSC a suitable candidate for various therapeutic strategies. In addition, cells derived from dental tissue can be transformed into induced pluripotent stem cells to customize cell-based regenerative approaches [2].…”

Section: Introductionmentioning

confidence: 99%

State-of-the-Art of the Clinical use of Stem Cells from Deciduous Teeth: A Review

Souza

Zotarelli-Filho

Kassis

et al. 2021

MedNEXTJ Med Health Sci

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Introduction: In recent years, mesenchymal stem cells derived from dental tissue have gained popularity for applications in tissue engineering and regenerative medicine. Objective: To present the main considerations about stem cells of primary teeth, as well as the importance of their clinical application. Methods: Experimental and clinical studies were included (case reports, retrospective, prospective and randomized studies), following the rules of systematic review-PRISMA. The search strategy was performed on Medline / Pubmed, Web Of Science, ScienceDirect Journals (Elsevier), Cochrane Library, Harvard Library, Scopus, and OneFile (Gale). Major findings and Conclusion: Dental pulp stem cells from primary teeth have very promising properties and potential for bone TE; however, based on these findings and the well-known restrictions in stem cell research, it may be beneficial to at least standardize the methods currently used in animal models based on the bone defect to heal, in order to achieve a higher level of reliability results, enabling safe clinical use.

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“…These cells were initially described as one specific type of ecto-mesenchymal stromal cells, because they fulfill the morphological and marker expression criteria for their classification as such, and they can also be induced to differentiate to diverse connective tissue lineages when they were maintained in culture media containing 10-20% fetal serum [10,11]. The use of hDPSCs created substantial interest in the tissue engineering field because of their high capacity for multilineage differentiation [12,13]. However, it soon became apparent that hDPSCs were not a conventional type of MSCs, and that their capacities exceeded those of a typical MSC.…”

Section: Introductionmentioning

confidence: 99%

Vasculogenesis from Human Dental Pulp Stem Cells Grown in Matrigel with Fully Defined Serum-Free Culture Media

et al. 2020

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The generation of vasculature is one of the most important challenges in tissue engineering and regeneration. Human dental pulp stem cells (hDPSCs) are some of the most promising stem cell types to induce vasculogenesis and angiogenesis as they not only secrete vascular endothelial growth factor (VEGF) but can also differentiate in vitro into both endotheliocytes and pericytes in serum-free culture media. Moreover, hDPSCs can generate complete blood vessels containing both endothelial and mural layers in vivo, upon transplantation into the adult brain. However, many of the serum free media employed for the growth of hDPSCs contain supplements of an undisclosed composition. This generates uncertainty as to which of its precise components are necessary and which are dispensable for the vascular differentiation of hDPSCs, and also hinders the transfer of basic research findings to clinical cell therapy. In this work, we designed and tested new endothelial differentiation media with a fully defined composition using standard basal culture media supplemented with a mixture of B27, heparin and growth factors, including VEGF-A165 at different concentrations. We also optimized an in vitro Matrigel assay to characterize both the ability of hDPSCs to differentiate to vascular cells and their capacity to generate vascular tubules in 3D cultures. The description of a fully defined serum-free culture medium for the induction of vasculogenesis using human adult stem cells highlights its potential as a relevant innovation for tissue engineering applications. In conclusion, we achieved efficient vasculogenesis starting from hDPSCs using serum-free culture media with a fully defined composition, which is applicable for human cell therapy purposes.

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Dental Tissue-Derived Mesenchymal Stem Cells: Applications in Tissue Engineering

Cited by 48 publications

References 0 publications

Exosomes from oral tissue stem cells: biological effects and applications

Exosomes from oral tissue stem cells: biological effects and applications

State-of-the-Art of the Clinical use of Stem Cells from Deciduous Teeth: A Review

Vasculogenesis from Human Dental Pulp Stem Cells Grown in Matrigel with Fully Defined Serum-Free Culture Media

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