Dentin demineralization: Effects of dentin depth, pH and different acids

Marshall, Grayson W.; Inai, Norimichi; Magidi, I-Chien Wu; Balooch, M.; Kinney, J.H.; Tagami, J; Marshall, Sally J.

doi:10.1016/s0109-5641(97)80104-2

Cited by 62 publications

(56 citation statements)

References 12 publications

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“…The two sectioned pieces then were bonded together with cyanoacrylate (Adhesive QX-4; MDS Products, Inc., Anaheim, California), which served subsequently as a reference layer for the etching experiments, as previously described. 15,16 Each rebonded specimen then was sectioned perpendicular to the dentin tubules in the regions of interest (sclerotic or normal root dentin) to provide a 1-1.5-mm-thick disk. The surface of the disk was polished to 0.05 m with alumina powder after it had been embedded in auto-curing polymethylmethacrylate resin and ultrasonicated to remove the remnant smear layer.…”

Section: Sample Preparationmentioning

confidence: 99%

Citric acid etching of cervical sclerotic dentin lesions: An AFM study

Marshall

Chang

Saeki

et al. 2000

J. Biomed. Mater. Res.

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Atomic force microscopy (AFM) has been used to determine microstructural changes, etching rates of peritubular dentin, and intertubular dentin recession during demineralization in dilute acidic solutions. These studies have not included many forms of altered dentin, including noncarious sclerotic root dentin associated with Cl V (abfraction) lesions. We sought to determine microstructural changes and recession rates during demineralization in citric acid (pH 2.5, 0.018M) for the transparent/sclerotic zone. Highly polished dentin disks were prepared from teeth with noncarious C1 V lesions (n = 3) and compared with normal root dentin (n = 3). Samples were etched at 5-s intervals for 1 min and at longer intervals up to 30 min. The depth changes in various portions of the dentin with respect to the reference layer were measured and changes in microstructure observed in solution in the wet cell of the AFM. In sclerotic dentin, most tubule lumens were occluded with crystalline deposits that etched more slowly than the other dentin components, but etching rates could not be determined due to their irregular geometry. The intertubular dentin recession quickly reached a plateau after a depth change of <1 microm for all dentin types, in agreement with prior work. Mixed linear regression models indicated an important difference between the etching of sclerotic intertubular dentin and that of non-sclerotic root dentin that became apparent after 600 s (p = 0.037). The sclerotic intertubular dentin underwent less depth change at the plateau (558 nm) compared to normal root dentin (744 nm). In addition, normal root dentin underwent significantly greater recession than coronal dentin (p = 0.002). The results of this study indicate that intertubular sclerotic dentin from Cl V lesions etches differently than normal root dentin, and this may help explain the difficulties in restoring such lesions with current bonding procedures.

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Section: Sample Preparationmentioning

confidence: 99%

Citric acid etching of cervical sclerotic dentin lesions: An AFM study

Marshall

Chang

Saeki

et al. 2000

J. Biomed. Mater. Res.

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“…Um fator importante para a efetividade dos condicionadores ácidos é a concentração do ácido fosfórico, que, em última análise, também influencia o seu pH 12 . Todos os condicionadores ácidos utilizados apresentaram ácido fosfórico em concentrações que variavam de 30 a 40%, quantidade que se tem demonstrado efetiva na produção de padrão adequado de condicionamento da dentina 6,13,14 .…”

Section: Discussionunclassified

Influência da marca do condicionador ácido na resistência de união da resina composta à dentina

Passos

Santos

Rodrigues

et al. 2012

Rev. odontol. UNESP

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ResumoIntrodução: Apesar da importância da etapa de condicionamento ácido nos procedimentos adesivos, pouco se sabe sobre o efeito da marca do condicionador ácido sobre a união do adesivo à dentina. Objetivo: Este estudo avaliou o efeito de diferentes condicionadores ácidos na união da resina composta à dentina. Material e método: Vinte e cinco incisivos bovinos tiveram a coroa dividida em duas hemissecções. As superfícies vestibulares foram desgastadas com lixa carbeto de silício (SiC) #180 até a exposição de dentina, seguida por lixas SiC #600 para padronização da smear layer. As amostras foram aleatoriamente alocadas em cinco grupos (n = 10), de acordo com marca do condicionador ácido: 3M ESPE, Ultradent, Dentsply, Villevie e DFL. Apenas um sistema de união convencional de dois passos (Ambar, FGM) foi utilizado. Matrizes de silicone foram utilizadas na confecção de três cilindros de resina composta em cada amostra. Após 24 horas, os cilindros foram testados sob carga de cisalhamento até a falha. Os dados foram analisados com ANOVA a um critério e teste de Tukey (α = 0,05). O modo de falha foi avaliado em microscopia óptica e os dados submetidos ao Teste Qui-quadrado (α = 0,05). Resultado: Não houve diferença estatística na resistência de união obtida entre os condicionadores ácidos avaliados. Houve predomínio de falhas adesivas, exceto para os condicionadores da Dentsply e DFL, que demonstraram similaridade entre falhas adesivas e mistas. Conclusão: As diferentes marcas de condicionadores ácidos avaliados não influenciaram a resistência de união de resina composta à dentina.Descritores: Adesivos dentinários; resistência ao cisalhamento; ataque ácido dentário. AbstractIntroduction: Despite the importance of the acid etching step on bonding procedures, little is known about the acid etchant brand effect on bond strength of adhesive to dentin. Objective: The aim of this study was to evaluate the effect of different acid etchants at the bond strength of composite resin to dentine. Material and method: Twenty five bovine incisors had their crown sectioned in two hemi-sections. The buccal surfaces were grounded with #180 silicon carbide (SiC) abrasive paper to exposure of dentin, followed by #600 SiC paper to standardize the smear layer. The samples were randomly allocated into five groups (n = 10) according to acid etchant brands: 3M ESPE, Ultradent, Dentsply, Villevie and DFL.Only a two-step etch-and-rinse adhesive system (Ambar, FGM) was used. Silicon molds were used to build-up three resin composite cylinder per sample. After 24 hours, cylinders were submitted to shear load until the failure. Data were analyzed by one way ANOVA and Tukey`s test (α = 0.05). The failure mode was evaluated under optical microscopy and the data were submitted to Chi-square test (α = 0.05). Result: There was no statistical difference on bond strength obtained between the acid etchants evaluated. There was predominance of adhesive failures, except for etchants of Dentsply and DFL that showed similarity between mixed and adhesive fail...

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“…40 The main decalcification was located at the peritubular area, 44 with less acid penetration at the intertubular aspect.…”

Section: Figure 3a: Backscattered Fe-sem Image Of Specimen Conditionementioning

confidence: 97%

“…39,[40][41][42] After this procedure, 3 layers can be distinguished: first, a superficial smeared collagen layer; second, an intermediate, densely packed fibrilar layer and third, a deeper area with some scattered mineral crystals and a few randomly exposed collagen fibrils. 36 The size of the porosities in dentin has been estimated to be about 10-50 nm and are filled with water or dentinal fluids.…”

Section: Figure 3a: Backscattered Fe-sem Image Of Specimen Conditionementioning

confidence: 99%

“…45 For CSE, increasing conditioning time did not affect the depth of decalcification (from 1.2 µm to 1.4 µm), which is in contrast with ADP, which displayed decalcification (ranging from 1.9 µm to 3.5 µm) similar to the total-etch adhesives tested (1.9 µm to 3.8 µm). As the depth of decalcification increases with the decrease in pH, 40 it would be more difficult for the hydrophilic monomers to penetrate into deeper areas of the decalcified dentin. [49][50] However, a correlation between the different pH and depth of decalcification was not observed regarding the percentage of nanoleakage within the hybrid layer, as stated in Figure 1.…”

Section: Figure 5a: Secondary Electron Fe-sem Image Of Specimen Restomentioning

confidence: 99%

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Effect of Dentin Conditioning Time on Nanoleakage

Duarte

Perdigão

Lopes

2006

Operative Dentistry

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Operative Dentistry, 2006, 30-4, 500-511 S Duarte Jr • J Perdigão • MM Lopes Clinical RelevanceIncreasing dentin conditioning time did not affect nanoleakage for any of the dentin adhesives used in this study. SUMMARYObjectives: To study the nanoleakage pattern in the dentin hybrid layer by using different dentin adhesives. The null hypotheses tested in this study were: 1) dentin conditioning time does not affect nanoleakage within the hybrid layer; 2) the type of dentin adhesive used does not affect nanoleakage. Methods: Standardized Class V cavities were prepared in 30 intact human molars on the buccal and lingual surfaces. The specimens were randomly assigned to 2 total-etch dentin adhesives ( Upon restoration of the Class V cavities with the proprietary resin composite, the specimens were isolated with nail polish except for a 2.0-mm rim around the restoration, and they were immersed in 50 wt% ammoniacal silver nitrate solution (pH=9.5) for 24 hours followed by 8 hours of immersion in photo-developing solution to reduce the silver ions to metallic silver. The specimens were fixed, dehydrated and processed for FESEM and TEM. Silver penetration was measured along the cervical wall, and data were analyzed with Kruskal-Wallis non-parametric tests at a significance level of 95%. Results: There were no statistically significant differences among the experimental groups for the factor "conditioning time" (p>0.926). There were significant differences for the variable "dentin adhesive" (p<0.0001). The least amount of nanoleakage within the hybrid layer occurred with CSE, while ONS resulted in the greatest penetration of silver ions. The adhesives OPS and APL ranked in the intermediary subset. Under TEM, all adhesives resulted in some degree of nanoleakage within the hybrid layer. Both spotted/reticular and water-tree nanoleakage patterns were observed. Significance: Longer conditioning times did not increase nanoleakage within the hybrid layer. Nanoleakage varied with the type of adhesive used.

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Dentin demineralization: Effects of dentin depth, pH and different acids

Cited by 62 publications

References 12 publications

Citric acid etching of cervical sclerotic dentin lesions: An AFM study

Citric acid etching of cervical sclerotic dentin lesions: An AFM study

Influência da marca do condicionador ácido na resistência de união da resina composta à dentina

Effect of Dentin Conditioning Time on Nanoleakage

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