Dentin matrix protein 1 expression during osteoblastic differentiation, generation of an osteocyte GFP-transgene

Kalajzić, Ivo; Braut, Alen; Guo, Dayong; Jiang, Xi; Kronenberg, Mark S.; Mina, M.; Harris, Marie A.; Harris, S. E.; Rowe, David W.

doi:10.1016/j.bone.2004.03.006

Cited by 202 publications

(221 citation statements)

References 24 publications

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“…Dentin matrix protein-1 (DMP1)-green fluorescent protein (GFP) mice, that express GFP under the control of 8 kb of the Dmp1 promoter region and 4439 bp of the first exon, first intron and part of the second exon, to restrict GFP expression to osteocytes (Kalajzic et al 2004), were obtained from the colony of Dr Hong Zhou, ANZAC Research Institute (Sydney). The animals were housed in a 12 h light:12 h darkness cycle with food and water provided ad libitum.…”

Section: Animal Experimentsmentioning

confidence: 99%

Decline in calcitonin receptor expression in osteocytes with age

Gooi

Chia

Walsh

et al. 2014

Journal of Endocrinology

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We have previously shown that co-administration of the transient osteoclast inhibitor, salmon calcitonin (sCT), blunts the anabolic effect of parathyroid hormone (PTH) in young rats and increases osteocytic expression of the bone formation inhibitor sclerostin (Sost). To determine whether this also occurs in adult animals, we co-administered sCT with PTH to 6-month-old sham-operated (SHAM) and ovariectomised (OVX) rats. While sCT reduced the stimulatory effect of PTH on serum amino-terminal propeptide of type 1 procollagen levels, in contrast to its influence in young rats, sCT did not reduce the anabolic effect of PTH on femoral bone mineral density, tibial trabecular bone volume or bone formation rate in 6-month-old SHAM or OVX rats. Quantitative real-time PCR analysis of femoral metaphyses collected 1 and 4 h after a single PTH injection confirmed a significant increase in mRNA levels for interleukin 6 (Il6) and ephrinB2 (EfnB2), and a significant reduction in Sost and dentin matrix protein-1 (Dmp1) in response to PTH. However, in contrast to observations in young rats, these effects were not modified by co-administration of sCT, nor did sCT significantly modify Sost, Dmp1, or matrix extracellular phosphoglycoprotein (Mepe) mRNA levels. Furthermore, while CT receptor (CTR) mRNA (Calcr) was readily detected in GFPC osteocytes isolated from young (3-week-old) DMP1-GFP mice, Calcr levels in osteocytes declined as mice aged, reaching levels that were undetectable in long bone at 49 weeks of age. These data indicate that osteocyte-mediated responses to CT are most likely to be of physiological relevance in young rodents.

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Section: Animal Experimentsmentioning

confidence: 99%

Decline in calcitonin receptor expression in osteocytes with age

Gooi

Chia

Walsh

et al. 2014

Journal of Endocrinology

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“…Whole Mount Calvaria Immunostaining-For whole-mount bone immunostaining for ␣-tubulin, transgenic mice expressing the topaz variant of enhanced green fluorescent protein (eGFP-tpz) targeted to the osteocyte were used (45). These mice were kindly provided by Dr. David Rowe (University of Connecticut Health Center, Farmington, CT 06030).…”

Section: Measurement Of Alkaline Phosphatase Activity and Mineralizatmentioning

confidence: 99%

“…In these mice GFP is expressed almost exclusively in osteocytes and preosteocytes. This transgenic mouse model, therefore, provides an extremely useful tool for imaging of osteocytes either in situ within the bones or in cell cultures (45)(46)(47).…”

Section: Measurement Of Alkaline Phosphatase Activity and Mineralizatmentioning

confidence: 99%

Cilia-like Structures and Polycystin-1 in Osteoblasts/Osteocytes and Associated Abnormalities in Skeletogenesis and Runx2 Expression

Xiao

Zhang

Mahlios

et al. 2006

Journal of Biological Chemistry

234

259

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We examined the osteoblast/osteocyte expression and function of polycystin-1 (PC1), a transmembrane protein that is a component of the polycystin-2 (PC2)-ciliary mechano-sensor complex in renal epithelial cells. We found that MC3T3-E1 osteoblasts and MLO-Y4 osteocytes express transcripts for PC1, PC2, and the ciliary proteins Tg737 and Kif3a. Immunohistochemical analysis detected cilia-like structures in MC3T3-E1 osteoblastic and MLO-Y4 osteocyte-like cell lines as well as primary osteocytes and osteoblasts from calvaria. Pkd1 m1Bei mice have inactivating missense mutations of Pkd1 gene that encode PC1. Pkd1 m1Bei homozygous mutant mice demonstrated delayed endochondral and intramembranous bone formation, whereas heterozygous Pkd1 m1Bei mutant mice had osteopenia caused by reduced osteoblastic function. Heterozygous and homozygous Pkd1 m1Bei mutant mice displayed a gene dose-dependent decrease in the expression of Runx2 and osteoblastrelated genes. In addition, overexpression of constitutively active PC1 C-terminal constructs in MC3T3-E1 osteoblasts resulted in an increase in Runx2 P1 promoter activity and endogenous Runx2 expression as well as an increase in osteoblast differentiation markers. Conversely, osteoblasts derived from Pkd1 m1Bei homozygous mutant mice had significant reductions in endogenous Runx2 expression, osteoblastic markers, and differentiation capacity ex vivo. Co-expression of constitutively active PC1 C-terminal construct into Pkd1 m1Bei homozygous osteoblasts was sufficient to normalize Runx2 P1 promoter activity. These findings are consistent with a possible functional role of cilia and PC1 in anabolic signaling in osteoblasts/osteocytes.

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“…Mice expressing green fluorescent protein under the control of the dentin matrix protein 1 promoter (DMP1-GFP) in a C57BL/6 background were previously described (15). All mice were fed a regular diet and water ad libitum and maintained on a 12-h light/dark cycle.…”

Section: Methodsmentioning

confidence: 99%

High Bone Mass in Mice Lacking Cx37 Because of Defective Osteoclast Differentiation

Pacheco‐Costa

Hassan

Reginato

et al. 2014

Journal of Biological Chemistry

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Background: Connexin proteins are essential for cell differentiation, function, and survival. Results: Global deletion of Cx37 results in increased bone mass caused by reduced osteoclast maturation. Conclusion: Our findings demonstrate a previously unrecognized role of Cx37 in bone homeostasis in vivo. Significance: Therapeutic approaches to increase bone mass might be developed by interfering with Cx37 function.

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Dentin matrix protein 1 expression during osteoblastic differentiation, generation of an osteocyte GFP-transgene

Cited by 202 publications

References 24 publications

Decline in calcitonin receptor expression in osteocytes with age

Decline in calcitonin receptor expression in osteocytes with age

Cilia-like Structures and Polycystin-1 in Osteoblasts/Osteocytes and Associated Abnormalities in Skeletogenesis and Runx2 Expression

High Bone Mass in Mice Lacking Cx37 Because of Defective Osteoclast Differentiation

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