Dentinal Tubule Penetration of a Calcium Silicate-Based Root Canal Sealer Using a Specific Calcium Fluorophore

Coronas, Viviane Siqueira; Villa, Natália; Nascimento, Angela Longo do; Duarte, Pedro Henrique Marks; Rosa, Ricardo Abreu da

doi:10.1590/0103-6440202002829

Cited by 21 publications

(22 citation statements)

References 25 publications

(38 reference statements)

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“…No reliable data exist about the penetration depth of endodontic sealers others than from studies where sealers were labelled with rhodamine B [ 7 , 8 ]. Studies using a calcium marker indicated that depth of apatite crystal precipitation in the dentinal tubules is more inferior than expected from previous studies [ 4 , 7 , 9 ]. However, this technique is also not yet validated, and no method is available to evaluate the sealer penetration by direct detection of the sealer in the dentinal tubules underneath the specimen’s surface.…”

Section: Introductionmentioning

confidence: 62%

Minimal Dentinal Tubule Penetration of Endodontic Sealers in Warm Vertical Compaction by Direct Detection via SEM Analysis

Schmidt¹,

Schäfer²,

Bürklein³

et al. 2021

JCM

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Sealer staining using rhodamine B dye to investigate the penetration depth of endodontic sealers was proven unsuitable for this purpose. This study aimed to investigate the sealer penetration depth into dentinal tubules by scanning electron microscopy (SEM). Root canals of 52 human upper central incisors were instrumented using the ProTaper Gold NiTi system (Dentsply Sirona, York, PA, USA) up to size F3. After irrigation with sodium hypochlorite and citric acid combined with ultrasonic activation, the root canals were either filled using the epoxy resin sealer AH Plus (Dentsply Sirona) or the calcium silicate-based sealer Total Fill BC Sealer HiFlow (TFHF, FKG Dentaire, La Chaux-de-Fonds, Switzerland) by warm vertical compaction. Root slices of 1 mm thickness were obtained at 2 to 3, 5 to 6 and 8 to 9 mm from the apex. The root slices were investigated for sealer penetration into the dentinal tubules using SEM according to four root quadrants (buccal, mesial, oral, distal). Statistical analysis was performed by the Kruskal-Wallis test (p = 0.05) as data were not normally distributed according to the Shapiro-Wilk test. AH Plus penetrated significantly deeper into the dentinal tubules compared to TFHF at each root level (p < 0.05). Dentinal sealer penetration was deeper in the bucco-oral direction compared to the mesio-distal direction. AH Plus penetrated deeper into dentinal tubules than TFHF. Warm vertical compaction exerting high pressure on the root canal filling material is not able to press sealers deep into dentinal tubules as penetration depth values did not exceed a mean of 110 µm in SEM.

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Section: Introductionmentioning

confidence: 62%

Minimal Dentinal Tubule Penetration of Endodontic Sealers in Warm Vertical Compaction by Direct Detection via SEM Analysis

Schmidt¹,

Schäfer²,

Bürklein³

et al. 2021

JCM

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“…Similar to several previous studies, bioceramic sealers associated with rhodamine B were used in this study (Akcay et al, 2016; El Hachem et al, 2019; Keleş et al, 2018; Sigadam et al, 2020). Furtado et al (2020) stated that bioceramic sealers should not be used associated with rhodamine B, because the water necessary during setting of the sealer may attach to the hydrophilic rhodamine B instead of the bioceramic sealer (Coronas et al, 2020). Therefore, the use of bioceramic sealers associated with rhodamine B may have affected the results of this study.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the effect of different chelation solutions on penetration of resin‐based and bioceramic sealers with a novel method

Uysal

Kotan

Güneşer

et al. 2021

Microscopy Res & Technique

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This study aimed to investigate the effects of different chelation solutions on the penetration of resin‐based and bioceramic root canal sealers into dentinal tubules using a device that assembles conventional microplate detection with automated digital microscopy. Eighty‐four single‐rooted teeth were prepared with 30.07 nickel‐titanium instruments and divided into four groups of 21 according to the final irrigation solutions used: saline (control), ethylenediaminetetraacetic acid (EDTA), maleic acid (MA), and etidronic acid (HEBP). The samples were obturated with AH Plus, MTA Fillapex, or EndoSequence BC Sealer using the single‐cone technique. The roots were then sectioned horizontally at 2, 6, and 10 mm and examined using a Cytation 5 reader and Gen5 software. Data were compared using two‐way analysis of variance followed by univariate analyses and Tukey's multiple comparison test (p < .05). The mean penetration depth in the apical thirds was higher in the MA group than in the other groups (p = .013). The mean depth in the middle and coronal thirds was almost equal in the EDTA and HEBP groups. The mean depth was significantly higher in the coronal thirds of the root canals than in any other section in all final irrigation solution groups (p < .001). EndoSequence BC Sealer displayed higher penetration depth than AH Plus and MTA Fillapex, although the differences between the examined sealers were not statistically significant. The chelation solutions increased root canal sealer penetration into the dentinal tubule.

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“…The obtained results presented statistically significant differences in some testing conditions, supporting the n in each group was sufficient. Comparison between the different root thirds (apical, middle and coronal) provided essential information regarding the different anatomical characteristics and material behavior in each area, as explored in several papers [28][29][30][31]. In this study, we focused on the apical and middle thirds, since the sealer penetration in these areas is fundamental to the success of endodontic treatment.…”

Section: Discussionmentioning

confidence: 99%

“…Instead of rhodamine B, Fluo-3 is a nonfluorescent compound that binds selectively to the calcium present in calcium silicate-based sealer. The presence of these ions increases fluorescence, decreasing false-positive results when compared to other dyes, namely rhodamine B [28,34].…”

Section: Discussionmentioning

confidence: 99%

Effect of Different Irrigation Solutions on the Diffusion of MTA Cement into the Root Canal Dentin

et al. 2020

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(1) Aim: This study aims to analyze the in vitro infiltration of a silicate root canal sealer into dentinal tubules after using different endodontic irrigating solutions. (2) Methods: Twenty-nine teeth with single roots were separated into three groups according to the final irrigation protocol: G1 n = 10) = 17% EDTA (ethylenediaminetetraacetic acid) + 3.0% sodium hypochlorite (NaOCl), G2 (n = 10) = 17% EDTA + 2.0% chlorhexidine and G3 (Control group, n = 9) = 17% EDTA + saline solution. Root canals were filled using cold lateral compaction technique with MTA Fillapex sealer and gutta-percha. The sealer was labeled with rhodamine B. The teeth were segmented at the middle and third apical sections, which were visualized using 10× confocal laser microscopy to determine the sealer penetration percentage. (3) Results: In the apical section, no statistically significant differences were found between the groups regarding sealer penetration. In the middle section, Group 1 obtained the highest percentage, and Group 2 the lowest (p = 0.004). Group 1 also presented statistically significant differences in the Control Group (p = 0.031) and had close sealer penetration values. Meanwhile, the Control Group (p = 0.023) and Group 2 (p = 0.029) revealed a significant decrease of sealer penetration between the apical and middle sections. (4) Conclusion: The obtained results support that final irrigation with NaOCl promoted similar sealer penetration in the apical and middle sections. On the other hand, a significant decrease in the sealer penetration of the middle section was observed for the chlorhexidine and saline groups. Compared to other irrigant solutions, NaOCl promotes more uniform sealer penetration, which can correlate with better sealing and, consequently, higher endodontic treatment success.

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Dentinal Tubule Penetration of a Calcium Silicate-Based Root Canal Sealer Using a Specific Calcium Fluorophore

Cited by 21 publications

References 25 publications

Minimal Dentinal Tubule Penetration of Endodontic Sealers in Warm Vertical Compaction by Direct Detection via SEM Analysis

Minimal Dentinal Tubule Penetration of Endodontic Sealers in Warm Vertical Compaction by Direct Detection via SEM Analysis

Investigation of the effect of different chelation solutions on penetration of resin‐based and bioceramic sealers with a novel method

Effect of Different Irrigation Solutions on the Diffusion of MTA Cement into the Root Canal Dentin

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