Deoxycytidyl transferase activity of yeast REV1 protein

Nelson, John; Lawrence, Christopher W.; Hinkle, David C.

doi:10.1038/382729a0

Cited by 533 publications

(497 citation statements)

References 10 publications

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“…Rev1 has a deoxycytidyltransferase activity that is directed opposite various damaged bases, as well as abasic sites and normal DNA 66,67 . The Rev1 protein contains an N-terminal domain that is unique among other Y family enzymes 46 (known as BRCA1 C-terminal (BRCT), which is involved in protein-protein interactions) and a C-terminal region that mediates interaction with other TLS polymerases 68 .…”

Section: Polη the Study Of Patients Affected By The Xeroderma Pigmenmentioning

confidence: 99%

DNA polymerases in adaptive immunity

Weill

Reynaud

2008

Nat Rev Immunol

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PrefaceTo cope with an unpredictable variety of potential pathogenic insults, the immune system must generate an enormous diversity of recognition structures, and it does so by making stepwise modifications of key genetic loci in each lymphoid cell. This proceeds through the action of lymphoid-specific proteins acting together with the general DNA-repair machinery of the cell. Strikingly, these general mechanisms are usually diverted from their normal functions, being used in rather atypical ways in order to privilege diversity over accuracy. In this Review, we focus on the contribution of a set of DNA polymerases discovered in the last decade to these unique DNA transactions.

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Section: Polη the Study Of Patients Affected By The Xeroderma Pigmenmentioning

confidence: 99%

DNA polymerases in adaptive immunity

Weill

Reynaud

2008

Nat Rev Immunol

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“…Rev1 is a deoxycytidyl transferase that inserts dCMP opposite G and certain lesions like abasic sites and guanine adducts [Nelson et al, 1996a;Waters et al, 2009]. Rev1 is proposed to act as a scaffold for multiple polymerases .…”

Section: Rev1mentioning

confidence: 99%

Mouse models of DNA polymerases

Menezes¹,

Sweasy²

2012

Environ and Mol Mutagen

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In 1956, Arthur Kornberg discovered the mechanism of the biological synthesis of DNA and was awarded the Nobel Prize in Physiology or Medicine in 1959 for this contribution, which included the isolation and characterization of Escherichia coli DNA polymerase I. Now there are 15 known DNA polymerases in mammalian cells that belong to four different families. These DNA polymerases function in many different cellular processes including DNA replication, DNA repair, and damage tolerance. Several biochemical and cell biological studies have provoked a further investigation of DNA polymerase function using mouse models in which polymerase genes have been altered using gene-targeting techniques. The phenotypes of mice harboring mutant alleles reveal the prominent role of DNA polymerases in embryogenesis, prevention of premature aging, and cancer suppression. Environ. Mol. Mutagen. 53:645-665, 2012. V V C 2012 Wiley Periodicals, Inc.

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“…It is not a polymerase, but a dCMP transferase, inserting a dCMP residue in a template-directed manner [18]. In the crystal structure, the incoming dCTP pairs with an arginine in the active site [19].…”

Section: Tls Polymerasesmentioning

confidence: 99%

Translesion synthesis in mammalian cells

Lehmann

2006

Experimental Cell Research

102

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DNA damage blocks the progression of the replication fork. In order to circumvent the damaged bases, cells employ specialised low stringency DNA polymerases, which are able to carry out translesion synthesis (TLS) past different types of damage. The five polymerases used in TLS in human cells have different substrate specificities, enabling them to deal with many different types of damaged bases. PCNA plays a central role in recruiting the TLS polymerases and effecting the polymerase switch from replicative to TLS polymerase. When the fork is blocked PCNA gets ubiquitinated. This increases its affinity for the TLS polymerases, which all have novel ubiquitin-binding motifs, thereby facilitating their engagement at the stalled fork to effect TLS.

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Deoxycytidyl transferase activity of yeast REV1 protein

Cited by 533 publications

References 10 publications

DNA polymerases in adaptive immunity

DNA polymerases in adaptive immunity

Mouse models of DNA polymerases

Translesion synthesis in mammalian cells

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