Deoxynivalenol reduces quality parameters and increases DNA damage in mice spermatozoa

Salahipour, Mahsa Hallaj; Hasanzadeh, Shapour; Malekinejad, Hassan; Razi, Mazdak; Farrokhi-Ardebili, Farhad

doi:10.1111/and.13238

Cited by 10 publications

(7 citation statements)

References 34 publications

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“…However, studies on the effects of DON on semen of other species have showed interesting results. In a recent study on mice sperm, Salahipour and coworkers observed a dose-dependent toxic effect of 2.5–10 μM DON with a reduction in sperm metabolic activity and membrane integrity, and an increase in lipid peroxidation rate and DNA damage [ 44 ]. By contrast, in our study, toxic effects of DON on boar semen were not observed at concentrations up to 50.6 μM, and we did not detect an effect on DNA integrity.…”

Section: Discussionmentioning

confidence: 99%

Individual and Combined In Vitro Effects of Deoxynivalenol and Zearalenone on Boar Semen

Tassis

Tsakmakidis

Nagl³

et al. 2020

Toxins

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Mycotoxins deoxynivalenol (DON) and zearalenone (ZEN) can negatively affect pig health. However, little is known about their effects on boar semen. We assessed the individual and combined effects of DON and ZEN on boar semen in vitro. In a pretrial, we determined the minimum dose (MiD) of each mycotoxin that induces a significant alteration of sperm progressive motility, as investigated using computer-assisted semen analysis (CASA). In the main trial, the individual and combined effects of each mycotoxin’s MiD on sperm motility and kinetics (CASA analysis), morphology (SpermBlue staining), viability (calcein-propidium iodide staining), membrane functional status (hypoosmotic swelling test), and chromatin integrity (acridine orange staining) were analyzed. Pretrial results suggested a MiD of 50.6 μM and 62.8 μM for DON and ZEN, respectively. In the main trial, DON and ZEN administered at MiD significantly affected CASA parameters (e.g., increase of immotile spermatozoa, reduction of progressive motile spermatozoa), decreased sperm viability, and affected sperm morphology (head abnormalities) and membrane functional status. DON and ZEN showed less than additive effects on most parameters tested and a synergistic effect on viability and on two CASA parameters. In conclusion, DON and ZEN showed individual and combined toxic effects on boar semen in vitro.

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Section: Discussionmentioning

confidence: 99%

Individual and Combined In Vitro Effects of Deoxynivalenol and Zearalenone on Boar Semen

Tassis

Tsakmakidis

Nagl³

et al. 2020

Toxins

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show abstract

“…Meanwhile, many studies have shown that DON can cause excessive ROS and induce DNA damage. For example, after 6 h of exposure to 10 µM DON, DNA damage in mouse sperm was significantly increased, accompanied by a 2.5-fold increase in MDA content [ 12 , 13 ]. However, it is not clear whether low dose sub-chronic DON exposure influences the liver’s antioxidant enzyme activities, DNA damage, and DNA repair ability.…”

Section: Introductionmentioning

confidence: 99%

The Protective Effect of Heme Oxygenase-1 on Liver Injury Caused by DON-Induced Oxidative Stress and Cytotoxicity

Meng

Wang

Liao

et al. 2021

Toxins

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Deoxynivalenol (DON) is a kind of Fusarium toxin that can cause a variety of toxic effects. Oxidative stress and DNA damage play a critical role in the toxicity of DON. However, previous studies focused more on acute toxicity in vivo/vitro models and lacked subchronic toxicity study in vivo. The potentially harmful effect of DON given at doses comparable to the daily human consumption in target organs, especially the liver, which is the main detoxification organ of DON, is also still not fully understood. Otherwise, Heme Oxygenase-1 (HO-1) has also reduced cell damage under the DON condition according to our previous study. Therefore, we used a rodent model that mimicked daily human exposure to DON and further explored its mechanism of toxic effects on liver tissue and Hepa 1–6 cell line. We also used adeno-associated virus (AAV)-modified HO-1 expressing by tail vein injection and constructed lentivirus-Hepa 1–6 cell line for mimicking HO-1 protective ability under the DON condition. The main results showed that both 30 d and 90 d exposures of DON could cause low-grade inflammatory infiltration around hepatic centrilobular veins. The reactive oxygen species (ROS) and 8-hydroxy-2 deoxyguanosine (8-OHdG) increased during DON exposure, indicating oxidation stress and DNA damage. Significantly, AAV-mediated liver-specific overexpression of HO-1 reduced DON-induced liver damage and indirectly protected the abilities of antioxidant enzyme/DNA damage repair system, while AAV-mediated silence of HO-1 produced the opposite effect. In addition, we found that overexpression of HO-1 could enhance autophagy and combined it with an antioxidant enzyme/DNA damage repair system to inhibit DON-induced hepatocyte damage. Altogether, these data suggest that HO-1 reduces the oxidative stress and DNA damage caused by DON sub-chronic exposure through maintaining DNA repair, antioxidant activity, as well as autophagy.

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“…DON was revealed to inhibit cell proliferation in different conditions (32,33). In the present study, the allicin both inhibited cell proliferation and aggravated the DON-induced barrier damages within the experimental condition, which may be attributed to the synergy-effects of DON and allicin in restraining DNA formation (34,35). Contrary to the roles of allicin in the DON-induced damage model, allicin successfully prevented the LPS-induced damages to intestinal barrier function, which prompts us to further validate its effects and explore potential mechanisms.…”

Section: Discussionmentioning

confidence: 48%

Allicin Improves Intestinal Epithelial Barrier Function and Prevents LPS-Induced Barrier Damages of Intestinal Epithelial Cell Monolayers

et al. 2022

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Gut barrier disruption is the initial pathogenesis of various diseases. We previously reported that dietary allicin improves tight junction proteins in the endoplasmic reticulum stressed jejunum. However, whether the allicin benefits the gut barrier within mycotoxin or endotoxin exposure is unknown. In the present study, IPEC-J2 cell monolayers within or without deoxynivalenol (DON) or lipopolysaccharide (LPS) challenges were employed to investigate the effects of allicin on intestinal barrier function and explore the potential mechanisms. Results clarified that allicin at 2 μg/mL increased the viability, whereas the allicin higher than 10 μg/mL lowered the viability of IPEC-J2 cells via inhibiting cell proliferation. Besides, allicin increased trans-epithelial electric resistance (TEER), decreased paracellular permeability, and enhanced ZO-1 integrity of the IPEC-J2 cell monolayers. Finally, allicin supplementation prevented the LPS-induced barrier damages via activating Nrf2/HO-1 pathway-dependent antioxidant system. In conclusion, the present study strongly confirmed allicin as an effective nutrient to improve intestinal barrier function and prevent bacterial endotoxin-induced barrier damages.

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Deoxynivalenol reduces quality parameters and increases DNA damage in mice spermatozoa

Cited by 10 publications

References 34 publications

Individual and Combined In Vitro Effects of Deoxynivalenol and Zearalenone on Boar Semen

Individual and Combined In Vitro Effects of Deoxynivalenol and Zearalenone on Boar Semen

The Protective Effect of Heme Oxygenase-1 on Liver Injury Caused by DON-Induced Oxidative Stress and Cytotoxicity

Allicin Improves Intestinal Epithelial Barrier Function and Prevents LPS-Induced Barrier Damages of Intestinal Epithelial Cell Monolayers

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