Deoxypodophyllotoxin; The Cytotoxic and Antiangiogenic Component from Pulsatilla koreana

Kim, Yong; Kim, Song Bae; You, Youngjae; Ahn, Byung Zun

doi:10.1055/s-2002-23140

Cited by 78 publications

(50 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Stock solutions were mixed to obtain the desired concentrations. The mixed stock solutions were then diluted in 50% methanol in the concentration ranges of 2 -200 µg/mL (2,4,8,20, 40, 80 and 200 µg/mL) for pulsatilloside E, anemoside B4, and cussosaponin C, and 5 -500 µg/mL (5, 10, 20, 50, 100, 200 and 500 µg/mL) for pulsatilla saponin H. All solutions were stored at 5 o C.…”

Section: Methodsmentioning

confidence: 99%

“…[2][3][4][5][6][7] Previous chemical and pharmacological investigations have revealed that triterpenoid saponins are its main biologically active components, exhibiting antitumor, cytotoxic, cognition-enhancing and other effects. [8][9][10] Recently, the saponin fraction of P. koreana was reported to improve memory impairments, and clinical trials for treatment of Alzheimer's disease using this fraction are in progress in Korea. 11,12 As noted above, triterpenoid saponins represent the major biological activities of P. koreana and these saponins could be used as chemical markers for quality control.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Simultaneous Determination of Triterpenoid Saponins from Pulsatilla koreana using High Performance Liquid Chromatography Coupled with a Charged Aerosol Detector (HPLC-CAD)

Yeom¹,

Suh²,

Youm³

et al. 2010

Bulletin of the Korean Chemical Society

View full text Add to dashboard Cite

Several triterpenoid saponins from root of Pulsatilla koreana Nakai (Ranunculaceae) were studied and their biological activities were reported. It is difficult to analyze triterpenoid saponins using HPLC-UV due to the lack of chromophores. So, evaporative light scattering detection (ELSD) is used as a valuable alternative to UV detection. More recently, a charged aerosol detection (CAD) has been developed to improve the sensitivity and reproducibility of ELSD. In this study, we developed and validated a novel method of high performance liquid chromatography coupled with a charged aerosol detector for the simultaneous determination of four triterpenoid saponins: pulsatilloside E, pulsatilla saponin H, anemoside B4 and cussosaponin C. Analytes were separated by the Supelco Ascentis ® Express C18 column (4.6 mm × 150 mm, 2.7 µm) with gradient elution of methanol and water at a flow rate of 0.8 mL/min at 30 o C. We examined various factors that could affect the sensitivity of the detectors, including various concentrations of additives, the pH of the mobile phase, and the CAD range. Linear calibration curves were obtained within the concentration ranges of 2 -200 µg/mL for pulsatilloside E, anemoside B4 and cussosaponin C, and 5 -500 µg/mL for pulsatilla saponin H with correlation coefficient (R 2 ) greater than 0.995. The limit of detection (LOD) and quantification (LOQ) were 0.04 -0.2 and 2 -5 µg/mL, respectively. The validity of the developed HPLC-CAD method was confirmed by satisfactory values of linearity, intra-and inter-day accuracy and precision. This method could be successfully applied to quality evaluation, quality control and monitoring of Pulsatilla koreana.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Triterpenoid Saponins from Pulsatilla koreana using High Performance Liquid Chromatography Coupled with a Charged Aerosol Detector (HPLC-CAD)

Yeom¹,

Suh²,

Youm³

et al. 2010

Bulletin of the Korean Chemical Society

View full text Add to dashboard Cite

show abstract

“…[2][3][4] Oleanane-type triterpenoid saponins, lupane-type triterpenoid saponins, quinones, phenylpropanoids, and flavonoid glycosides have been isolated from the root and aerial parts of P. koreana in previous phytochemical studies. [5][6][7][8][9] However, there have been few studies that have specifically aimed to identify compounds from the flowers of P. Koreana.…”

Section: Introductionmentioning

confidence: 99%

Flavonoid Glycosides from the Flowers ofPulsatilla koreanaNakai

et al. 2016

View full text Add to dashboard Cite

− Extraction and fractionation of Pulsatilla koreana flowers followed by, repeated open column chromatography for EtOAc and n-BuOH fractions yielded four flavonoid glycosides, namely, astragalin (1), tiliroside (2), buddlenoide A (3), and apigenin-7-O-(3''-E-p-coumaroyl)-glucopyranoside (4). The chemical structures of these flavonoid glycosides were elucidated on the basis of various spectroscopic methods including electronic ionization mass spectrometry (EI-MS), 1D NMR ( 1 H, 13C, DEPT), 2D NMR (gCOSY, gHSQC, gHMBC), and infrared (IR) spectrometry. This study represents the first report of the isolation of the flavonoid glycosides from the flowers of P. koreana.

show abstract

“…Also, PKE has been found to have prominent neuroprotective abilities to reverse scopolamine-induced cognitive impairment in rats (10). In addition, several studies have been reported that components of PKE were effective in preventing cancers cells including melanoma and ovarian cancer (12,13).…”

Section: Introductionmentioning

confidence: 99%

Apoptotic and anti-angiogenic effects of Pulsatillaï¿½koreana extract on hepatocellular carcinoma

Hong

Jung²,

Lee³

et al. 2011

Int J Oncol

View full text Add to dashboard Cite

Abstract. Chemoprevention through the use of food and plants has emerged as a novel approach to control various malignancies including cancer. Pulsatilla koreana extract (PKE) has been used to treat malaria and dysentery. The functions and effect of PKE in cancer treatment have been reported but with less information. In this study, we investigated the effect of PKE on the progression of hepatocellular carcinoma (HCC) cells and its mechanism. PKE strongly suppressed the growth of HCC cells in a dose-dependent manner. Apoptosis by PKE was observed by DAPI and TUNEL staining and accompanied with increases of cleaved PARP and caspase-3 in Huh-7 cells. Also, PKE decreased the expression of hypoxia-inducible factor (HIF-1α) and vascular endothelial growth factor (VEGF), and inhibited tube formation and migration of human umbilical vein endothelial cells (HUVECs). In addition, PKE potently suppressed in vivo neovascularization in a mouse Matrigel plug assay. Furthermore, in vivo study showed that PKE significantly inhibited tumor growth in a mouse xenograft model, and induced apoptosis by increasing the cleaved PARP and caspase-3. The expressions of Ki-67, VEGF, and CD31 in the tumor tissue were decreased by the treatment of PKE. Taken together, our study demonstrates that PKE not only induced apoptosis but also inhibited cell growth and angiogenesis of human HCC. We suggest that PKE is an effective chemotherapeutic candidate for cancer therapy against HCC.

show abstract

Deoxypodophyllotoxin; The Cytotoxic and Antiangiogenic Component from Pulsatilla koreana

Cited by 78 publications

References 5 publications

Simultaneous Determination of Triterpenoid Saponins from Pulsatilla koreana using High Performance Liquid Chromatography Coupled with a Charged Aerosol Detector (HPLC-CAD)

Simultaneous Determination of Triterpenoid Saponins from Pulsatilla koreana using High Performance Liquid Chromatography Coupled with a Charged Aerosol Detector (HPLC-CAD)

Flavonoid Glycosides from the Flowers ofPulsatilla koreanaNakai

Apoptotic and anti-angiogenic effects of Pulsatillaï¿½koreana extract on hepatocellular carcinoma

Contact Info

Product

Resources

About