Deoxyribonuclease activity of polyclonal IgGs: a putative serological marker in patients with spondyloarthritides

Abstract

Antibodies executing catalytic activity are referred to as antibody enzymes or short "abzymes" and may have diagnostic relevance. Abzymes with deoxyribonuclease (DNase) activity have been demonstrated in patients with autoimmune and infectious diseases. Despite several reports on the occurrence of DNase abzymes in systemic autoimmune rheumatic diseases, conclusive data about DNase activity of antibodies in patients with spondyloarthritides (SpAs) are lacking. In recent cross-sectional studies evaluating levels of IgG DNase activity in patients with psoriatic arthritis (PsA), reactive arthritis (ReA), and ankylosing spondylitis (AS), DNase activity of IgG has been assessed by the rivanol clot method and confirmed by agarose gel electrophoresis. Remarkably, levels of IgG DNase activity were significantly higher in sera of SpA patients than those in control subjects. In patients with PsA, ReA, and AS, a positive correlation of DNase IgG activity with synovitis, disease activity, and stage of spondylitis was observed, respectively. Given the involvement of autoimmune reactions in cytolysis and connective tissue degradation in PsA, ReA, and to a lesser extent in AS, abzymes might have an impact on the pathophysiology of SpAs. Detection of IgG DNase activity in patients suffering from SpA represents an exciting new research field and may assist in the differential diagnosis of SpA.

Immune cell transcript modules reveal leukocyte heterogeneity in synovial biopsies of seronegative spondylarthropathy patients

“…In our study, we do not observe CD8+ T cells levels within the synovia, implying that this cell type may not play as important a role in the local tissue as it does in the periphery. Additionally, a recent identification of abzymes (antibodies with catalytic activity) has been correlated to SpA [ 25 ], despite the absence of B cells within the local tissue. Indeed, extra-articular symptoms of AS and other SpA may involve such cell types; whether these cell types infiltrate the local environment of an extra-articular site of inflammation is an important question and remains unclear.…”

Enzymatic activity of blood serum, abzyme activity of IgG and IgA, free serum DNA and the formation of extracellular traps by neutrophils in patients with breast neoplasms

“…Нейтрофильные гранулоциты и нейтрофильные внеклеточные ловушки (НВЛ) являются постоянной структурой в микроокружении опухолей; их роль в онкогенезе неоднозначна [14,15,16] Согласно некоторым данным, они захватывают циркулирующие опухолевые клетки, способствуя метастазированию [17].…”

Heterogeneity of Polyclonal Immunoglobulins Nuclease Activity in Rheumatoid and Reactive Arthritis

“…Во-вторых, при ряде аутоиммунных заболеваний, таких как системная красная волчанка и ревматоидный артрит, кроме циркулирующей ДНК, в сыворотке крови появ-ляются антитела к ДНК и циркулирующие им-мунные комплексы с ДНК. Обнаружено повы-шение ДНКазной активности сыворотки крови при ревматоидном артрите, спондилоартритах [4,9]. Описано снижение ДНКазной активности сы-воротки при СКВ, в биологических моделях по-казано развитие клинических проявлений СКВ у мышей, дефицитных по гену ДНКазы I, у 2-х пациентов с СКВ обнаружены дефекты в гене, кодирующем ДНКазу I [10,16].…”

“…Наиболее высокие уровни ДНКазной активности иммунолобулинов установлены при ревматоидном артрите, псориатическом артрите, системной красной волчанке [9,18].…”