Deoxyribonucleic acid homologies among strains of <i>Bacteroides melaninogenicus</i> and related species

Steenbergen, T. J. M. van; Vlaanderen, C. A.; Graaff, J. de

doi:10.1111/j.1365-2672.1982.tb04685.x

Cited by 31 publications

(12 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The two strains selected for use as DNA probes in this study have been shown previously to represent at least two centers of variation among strains labeled Prevotella intermedia (7,27). In the previous studies, reciprocal hybridizations between members of the two groups of isolates revealed approximately 40% DNA-DNA homology, perhaps because of the lower stringency of the reassociation experiments.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Biochemical and Chemical Studies on Strains Designated Prevotella intermedia and Proposal of a New Pigmented Species, Prevotella nigrescens sp. nov.

Shah

Gharbia

1992

International Journal of Systematic Bacteriology

180

134

View full text Add to dashboard Cite

A total of 31 strains of Prevotella intermedia were subjected to DNA-DNA hybridization and were characterized by performing physiological tests and by performing a multilocus enzyme analysis, using malate dehydrogenase and glutamate dehydrogenase. All of the strains assigned to P. intermedia fermented glucose and sucrose, hydrolyzed starch but not esculin, and produced indole, acetic, isobutyric, isovaleric, and succinic acids as metabolic end products. The results of DNA reassociation experiments performed with the reference probe permitted separation of the strains into two well-defined homology groups. In addition, strains with DNAs that hybridized with DNA from strain ATCC 25611T ( ' I ' = type strain) had high levels of peptidase activity and cleaved lipid substrates (4-methylumbelliferyl laurate and 4-methylumbellifelyl elaidate). Multilocus enzyme electrophoresis revealed two electromorphic profiles, one characteristic of strain ATCC 25611T and the other characteristic of strain ATCC 335aT. We propose that a new species, Prevotella nigrescens, should be created for the genetically distinct group of strains that hybridized with strain ATCC 33563T. Strain ATCC 33563 is designated the type strain of P. nigrescens.The "black-pigmented bacteroides" have undergone major taxonomic revisions at both the generic and species levels within the last decade (for a review, see reference 14).

show abstract

Section: Resultsmentioning

confidence: 99%

“…1) and therefore provided a rapid way to identify unknown isolates. The identification of two DNA homology groups among human Prevotella intermedia isolates has been reported previously (7,27). These isolates have been subdivided into three serotypes, serotypes I, 11, and 111, by using monoclonal antibodies (4).…”

Section: Resultsmentioning

confidence: 99%

Biochemical and Chemical Studies on Strains Designated Prevotella intermedia and Proposal of a New Pigmented Species, Prevotella nigrescens sp. nov.

Shah

Gharbia

1992

International Journal of Systematic Bacteriology

180

134

View full text Add to dashboard Cite

show abstract

“…It has been shown that the group of black-pigmented Bacteroides strains is very heterogeneous on the basis of DNA base contents and DNA hybridization studies. Therefore, reciassification has been proposed by several authors (van Steenbergen, de Soet & de Graaff 1979, Coykendall, Kaczmarek & Slots 1980,, van Steenbergen, Vlaanderen & de Graaff 1981, van Steenbergen. Vlaanderen & de Graaff 1982b.…”

Section: Discussionmentioning

confidence: 99%

Histopathological effects in the palate of the rat induced by injection with different black‐pigmented Bacteroides strains

Roeterink

Steenbergen

Jong

et al. 1984

J of Periodontal Research

View full text Add to dashboard Cite

The present investigation was carried out in order to study the histopathological effect and virulence of different black‐pigmented Bacteroides species on both soft and hard tissues in an animal model. Male Wistar rats were injected in the palate next to the first right molar with 30 μl of a bacterial suspension containing 5 × 1010 viable cells per ml. To determine the difference in virulence the histopathological effects were studied five days after injection. All strains tested caused a submucosal infiltrate at the injection site, consisting of mononuclear cells, mainly lymphocytes, monocytes and fibroblasts. These inflammatory responses were accompanied by devitalization and resorption of bone. Simultaneously active osteoblasts and bone formation were observed. Strains of Bacteroides asaccharolyticus, Bacteroides melaninogenicus, Bacteroides corporis, and Bacteroides loescheii caused a less prominent devitalization of the palatinal bone than strains of Bacteroides gingivalis and Bacteroides intermedius. Strains of the latter species caused often a nasal infiltrate and abundant bone formation at the nasal side of the palatinal bone. B. asaccharolyticus and B. melaninogenicus scarcely caused signs of a reaction at the nasal side of the palatinal bone. In a second longitudinal study the histopathological effects of B. gingivalis strain HG 66 were investigated. The inflammation was characterized by a rapid influx of polymorphonuclear cells within 2 to 4 hours. These cells gradually diminished and were replaced by a mononuclear infiltrate. One of the most characteristic features accompanying the inflammation was the disappearance of the periosteum. After two days a recovery of periosteum was observed and the osteoblasts became active in new bone formation. Devitalization from the palatinal bone started about 8 hours after injection and could be seen until 10 days after injection. No inflammatory reaction or devitalization of bone could be observed 15 days after injection.

show abstract

“…Attempts to better classify this bacterial group have been made on the bases of physiologic characteristics (10), electrophoretic patterns of dehydrogenases (34), cellular fatty acid and sugar composition (3,4,18), lipid analysis (19,26), serology (15), and bacteriophage typing (2). The diversity of this bacterial group was further demonstrated in studies using 16S rRNA oligonucleotide cataloging (25), rRNA-DNA hybridization (12), DNA homology (11,36), and 5S rRNA comparative analysis (35). On the basis of this heterogeneity, Shah and Collins (30)(31)(32) proposed that the genus Bacteroides be divided into the following three genera: (i) Bacteroides sensu stricto, consisting of saccharolytic, nonpigmenting species such as the type species, B. fragilis, and its relatives; (ii) Prevotella, consisting of moderately saccharolytic, bile-sensitive, predominantly oral species, such as P. melaninogenica and P. intermedia; and (iii) Porphyromonas, generally consisting of asaccharolytic, black- L16484 L16489 L16486 M58762 M86695b L16495 L16493 L16494 L16497 L16496 L16490 L26102 L16491 L16492 L26103 L16475 L16477 L16478 L16476 L16465 L16467 L16466 L16483 L16487 L16468 L16481 L16469 L16470 L16471 L16479 L16480 L16474 L16472 L16482 L16473 L16488 L16498 (16).…”

mentioning

confidence: 99%

Phylogeny of Bacteroides, Prevotella, and Porphyromonas spp. and related bacteria

et al. 1994

View full text Add to dashboard Cite

The phylogenetic structure of the bacteroides subgroup of the cytophaga-flavobacter-bacteroides (CFB) phylum was examined by 16S rRNA sequence comparative analysis. Approximately 95% of the 16S rRNA sequence was determined for 36 representative strains of species of Prevotella, Bacteroides, and Porphyromonas and related species by a modified Sanger sequencing method. A phylogenetic tree was constructed from a corrected distance matrix by the neighbor-joining method, and the reliability of tree branching was established by bootstrap analysis. The bacteroides subgroup was divided primarily into three major phylogenetic clusters which contained most of the species examined. The first cluster, termed the prevotella cluster, was composed of 16 species of Prevotella, including P. melaninogenica, P. intermedia, P. nigrescens, and the ruminal species P. ruminicola. Two oral species, P. zoogkoformans and P. heparinolytica, which had been recently placed in the genus PrevoteUla, did not fall within the prevotella cluster. These two species and six species of Bacteroides, including the type species B. fragilis, formed the second cluster, termed the bacteroides cluster. The third cluster, termed the porphyromonas cluster, was divided into two subclusters. The first contained Porphyromonas gingivalis, P. endodontalis, P. asaccharolytica, P. circumdentaria, P. salivosa, [Bacteroides] [Bacteroides] splanchnicus fell just outside the three major clusters but still belonged within the bacteroides subgroup. On the basis of bootstrap analysis, the remaining species, [Bacteroides] putredinis, Rikenella microfusus, and two misclassified, free-living species of Cytophaga, appeared to form an additional subgroup separate from the bacteroides and other subgroups of the CFB phylum. However, on classification by single-base signatures, these species belonged within the bacteroides subgroup. Additional single-base signatures defined and differentiated clads within the bacteroides subgroup. With few exceptions, the 16S rRNA data were in overall agreement with previously proposed reclassifications of species of Bacteroides, Prevotella, and Porphyromonas. Suggestions are made to accommodate those species which do not fit previous reclassification schemes.The taxonomy of the "bacteroides" has undergone significant changes in the past few years. Species of this bacterial group were once loosely defined as obligately anaerobic, gram-negative, nonsporulating, pleomorphic rods. In addition, guanine-plus-cytosine contents of the DNA of the bacteroides were shown to range broadly from 28 to 61 mol% (10). Attempts to better classify this bacterial group have been made on the bases of physiologic characteristics (10), electrophoretic patterns of dehydrogenases (34), cellular fatty acid and sugar composition (3, 4, 18), lipid analysis (19, 26), serology (15), and bacteriophage typing (2). The diversity of this bacterial group was further demonstrated in studies using 16S rRNA oligonucleotide cataloging (25), rRNA-DNA hybridization (12), DNA homol...

show abstract

Deoxyribonucleic acid homologies among strains of Bacteroides melaninogenicus and related species

Cited by 31 publications

References 27 publications

Biochemical and Chemical Studies on Strains Designated Prevotella intermedia and Proposal of a New Pigmented Species, Prevotella nigrescens sp. nov.

Biochemical and Chemical Studies on Strains Designated Prevotella intermedia and Proposal of a New Pigmented Species, Prevotella nigrescens sp. nov.

Histopathological effects in the palate of the rat induced by injection with different black‐pigmented Bacteroides strains

Phylogeny of Bacteroides, Prevotella, and Porphyromonas spp. and related bacteria

Contact Info

Product

Resources

About