Deoxyribonucleic acid of cotton

Ergle, David R.; Katterman, Frank R. H.

doi:10.1104/pp.36.6.811

Cited by 39 publications

(6 citation statements)

References 11 publications

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“…The overall content in 5mC of plant genomes depends on genome size and content in repetitive DNA sequences. Yet, the Arabidopsis genome (120 Mb) contains only 6% of 5mC, whereas the maize genome (2,500 Mb) contains more than 25% (Ergle and Katterman 1961). Consistent with previous Wndings (Messeguer et al 1991), the 5mC content of the tomato genome (cv Ailsa craig) (950 Mb, Peterson et al 1996), ranged between 20 and 30% and depends both on tissues and developmental stages (Fig.…”

Section: Discussionsupporting

confidence: 83%

Tissue dependent variations of DNA methylation and endoreduplication levels during tomato fruit development and ripening

Teyssier

Bernacchia

Maury

et al. 2008

Planta

110

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Tomato fruit cells are characterized by a strong increase in nuclear ploidy during fruit development. Average ploidy levels increased to similar levels (above 50C) in two distinct fruit tissues, pericarp and locular tissue. However, ploidy profiles differed significantly between these two tissues suggesting a tissue-specific control of endoreduplication in tomato fruit. To determine possible relationships between endoreduplication and epigenetic mechanisms, the methylation status of genomic DNA from pericarp and locular tissue of tomato fruit was analysed. Pericarp genomic DNA was characterized by an increase of CG and/or CNG methylation at the 5S and 18S rDNA loci and at gyspsy-like retrotransposon sequences during fruit growth. A sharp decrease of the global DNA methylation level together with a reduction of methylation at the rDNA loci was also observed in pericarp during fruit ripening. Inversely, no major variation of DNA methylation either global or locus-specific, was observed in locular tissue. Thus, tissue-specific variations of DNA methylation are unlikely to be triggered by the induction of endoreduplication in fruit tissues, but may reflect tissue-specific ploidy profiles. Expression analysis of eight putative tomato DNA methyltransferases encoding genes showed that one chromomethylase (CMT) and two rearranged methyltransferases (DRMs) are preferentially expressed in the pericarp during fruit growth and could be involved in the locus-specific increase of methylation observed at this developmental phase in the pericarp.

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Section: Discussionsupporting

confidence: 83%

Tissue dependent variations of DNA methylation and endoreduplication levels during tomato fruit development and ripening

Teyssier

Bernacchia

Maury

et al. 2008

Planta

110

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“…In plant genomes, the overall 5-mC content differs between species and depends on genome size (Ergle & Katterman 1961). Within the same species, differences in methylation levels can occur in relation to developmental stage and between different organs/ tissues (Ruiz-García et al 2005, Lu et al 2008, Teyssier et al 2008.…”

Section: Discussionmentioning

confidence: 99%

Effects of light deficiency on genome methylation in Posidonia oceanica

Greco¹,

Chiappetta²,

Bruno³

et al. 2013

Mar. Ecol. Prog. Ser.

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Growth and distribution of seagrasses is closely related to undersea light quality and intensity. Currently, several factors linked to anthropogenic pressure, such as increased sediment runoff, resuspension of bottom sediments, and algae accumulations, reduce sub-surface light availability in most Mediterranean coastal areas, and are causing a general regression of Posidonia oceanica (L.) Delile meadows. We investigated whether plant capacity to adapt to light deficiency could be related to a repatterning of genome methylation in a natural population of P. oceanica subjected to low-light conditions. DNA methylation status was analyzed in shoot apical meristems and leaves of plants growing in a disturbed meadow, characterized by reduced light availability, versus plants growing in a preserved meadow. Two complementary approaches were applied, viz. the methylation-sensitive amplification polymorphism (MSAP) technique and im muno cytological detection of methylated sites on interphase nuclei. Genome hypermethylation was detected in the organs of plants growing at the disturbed site, indicating that de novo methylation occurred under light-related stress conditions. MSAP analysis revealed that changes in methylation status involved specific classes of genes with various functions, including light perception and harvesting genes such as PoPHYB and LHCB5. It is likely that epigenetic regulation of these 2 genes through methylation plays a role in the resistance and resilience of P. oceanica plants to critical light conditions. We propose that the status of genome methylation is a promising diagnostic tool for early detection of stress factors affecting P. oceanica meadows.

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“…By using sodiunm dodecyl sulfate as the extracting and deproteinizing agent (10), the preparations were isolated in highly polymerized form from 300 g samples of freshly decorticated embryos. Hydrolysis of the DNA isolates to the free purine and pyrimidine bases, chromatographlic separation, and spectrophotometric determination followed procedures described previously (10 mononucleotides were separated by descending paper chromatography (13) and eluted with 5 ml of IM potassium phosphate buffer of pH 7.0 at 370.…”

mentioning

confidence: 99%

“…Hydrolysis of the DNA isolates to the free purine and pyrimidine bases, chromatographlic separation, and spectrophotometric determination followed procedures described previously (10 mononucleotides were separated by descending paper chromatography (13) and eluted with 5 ml of IM potassium phosphate buffer of pH 7.0 at 370. The eluates were analyzed spectrophotometrically (10) and from the spectral data the nucleotide concentrations were calculated, using coefficients given by Elson et al (9).…”

mentioning

confidence: 99%