Deoxyribonucleic Acid Ploidy Studies in Choroidal Melanomas

Coleman, Kate; Baak, Jan P.H.; Dorman, Anthony; Mullaney, Joan; Curran, B.; Tiernan, Damien; Farrell, Michael J.; Fenton, M.; Leader, M.

doi:10.1016/s0002-9394(14)73592-5

Cited by 12 publications

(3 citation statements)

References 17 publications

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“…However, only 200 cells are used in image analysis compared with at least 10 000 in flow cytometry, techniques are complicated and time-consuming and many prepara tions are unsuccessful. 15 Another important difference from previously published studies is the sampling bias introduced by the majority of patients in the present study having medium or large tumours with a high proportion or a pure population of epithelioid cells. Small tumours more frequently have a low epithelioid content and a better prognosis for survival; therefore the present study represents a skewed population of patients who are already at a significant risk of developing metastases.…”

Section: Discussionmentioning

confidence: 66%

DNA index and % S phase fraction in posterior uveal melanoma: A 5 year prospective study of fresh tissue using flow cytometry

Richardson

Lawry

Rees

et al. 1997

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SUMMARYIn many different solid tumours the DNA couteut of cells has been reported to be associated with malig nancy. With one exception, studies using flow cytome try to estimate cellular DNA in uveal melanoma have used archival material. We report the findings of a prospective study using fresh uveal melanoma where the DNA index (DI) and percentage of cells in S phase (%SPF) have been determined by flow cytometry in 51 patients with a minimum follow-up of 5 years. Forty one (80%) of the tumours were large, 9 (18%) were medium and only 1 was small. Eight (16%) were epithelioid tumours, 41 (80%) were mixed cell and only 2 were spindle B cell tumours. Twenty-seven tumours were diploid, of which 10 (37%) developed metastases. Of the 24 aneuploid tumours, metastases developed in 6 (25%). The median %SPF was significantly reduced in patients with diploid tumours compared with aneuploid tumours (p

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Section: Discussionmentioning

confidence: 66%

DNA index and % S phase fraction in posterior uveal melanoma: A 5 year prospective study of fresh tissue using flow cytometry

Richardson

Lawry

Rees

et al. 1997

Eye

View full text Add to dashboard Cite

show abstract

“…IA is particularly useful in cell recognition and classification, and measurements of cell components. Comparing IA and FCM on uveal melanomas, tetraploidy was detected in tumours that were diploid by FCM 12. By IA it was found that all spindle A cells were diploid,7 12 and most tetraploid peaks were formed by epithelioid cells 12.…”

mentioning

confidence: 93%

“…Comparing IA and FCM on uveal melanomas, tetraploidy was detected in tumours that were diploid by FCM 12. By IA it was found that all spindle A cells were diploid,7 12 and most tetraploid peaks were formed by epithelioid cells 12. The lack of association of aneuploidy with epithelioid cell type may partly be explained by the application of a modified (five categories) classification (see Toti et al ).…”

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confidence: 99%