Deoxyribonucleic acid polymerase associated with feline leukemia and sarcoma viruses: Properties of the enzyme and its product

Roy-Burman, Pradip

doi:10.1002/ijc.2910070306

Cited by 14 publications

(4 citation statements)

References 16 publications

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“…I Reaction mixtures (0.2 ml) were cooled at 0 to 4 C after treatment at specified conditions; 100 ;tg (0.1 ml) of bovine serum albumin was added, and the proteins were precipitated by addition of 0.2 ml of cold 50% trichloroacetic acid. The acid-insoluble materials were collected on membrane filters (Millipore Corp.), washed, dried, and counted for radioactivity as described previously (21…”

Section: Discussionmentioning

confidence: 99%

“…cells were grown in 50% phosphate-free minimal essential medium containing 10% dialyzed fetal bovine serum in the presence of the 3H-labeled L-amino acid mixture (2 gCi/ml) and carrier-free [32P]phosphate (80 ACi/ml). Two changes of the medium were made at 24-h intervals, and the virus was purified from the pooled culture fluids by the published procedure (19,21).…”

Section: Methodsmentioning

confidence: 99%

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Phosphoproteins: structural components of oncornaviruses

Pal¹,

Roy-Burman²

1975

J Virol

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Oncornaviruses, which contain a virion-associated protein kinase, were found to possess phosphoproteins as virion structural components. One major phosphoprotein common to strains of laboratory and wild mouse oncornaviruses and a strain of feline leukemia virus was shown to be a polypeptide of about 12, 000 mol wt. In addition to this, the Kirsten strain of murine sarcoma virus contained a second major phosphoprotein of about 10, 000 mol wt, and mouse erythroblastosis virus contained a second major phosphoprotein that was either identical to or comigrated with the virion glycoprotein of about 74, 000 mol wt. The major phosphoprotein of RD-114 virus was found to be of about 16, 000 mol wt. The major phosphoamino acid of the 12, 000-mol wt polypeptide of the mouse erythroblastosis virus was identified as phosphoserine, and that of the 16, 000-mol wt polypeptide of the RD-114 virus was identified as phosphothreonine.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Phosphoproteins: structural components of oncornaviruses

Pal¹,

Roy-Burman²

1975

J Virol

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“…Two changes of the medium were made at 24-h intervals and the virus was purified from the pooled culture fluids by the published procedure (19,22). The pelleted virus was then disrupted in 20 mM Tris-hydrochloride (pH 7.4) containing 0.1% Nonidet P-40 at 0 C for 1 h and then treated with RNase (0.5 mg/ml, final concentration) at 37 C for 2 h. The solution was then brought to 8 M Gu .HCI and 0.3% ,8-mercaptoethanol, heated at 56 C for 45 min and applied on a Bio Gel A 5-m column (1.5 by 87 cm) equilibrated with 6 M Gu-HCl for fractionation of virion polypeptides (15,19).…”

Section: Labs Sodium Dodecyl Sulfate (Sds) Obtained Frommentioning

confidence: 99%

Comparative studies on the structural phosphoproteins of mammalian type C viruses

Pal¹,

McAllister²,

Gardner³

et al. 1975

J Virol

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The major phosphoprotein common to woolly monkey sarcoma virus, gibbon ape lymphosarcoma virus, and type C viruses of the lower mammalian species (mouse, rat, cat), with the exception of the endogenous cat virus (RD-114), is the polypeptide of about 12,000 molecular weight. The protein-phosphate bond in this polypeptide of several viruses is of the phosphoserine variety excepting gibbon ape virus, which contains both phosphoserine and phosphothreonine. The primary phosphoprotein of RD-114 virus and the endogenous baboon type C virus, on the other hand, is the polypeptide of about 15,000 molecular weight which contains phosphothreonine as its phosphoamino acid. A second major phosphoprotein of molecular weight of 10,000 is detected only in viruses genetically related to rat species including those derived from the RPL cell line, from Sprague-Dawley rat embryo cells, and the Kirsten mouse sarcoma virus which was recovered from a mouse erythroblastosis virus after in vivo propagation through rat. These phosphorylated polypeptides of molecular weight 15,000, 12,000, or 10,000 are present in the virion structure in several different but nonrandom phosphorylated states.

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“…Viruses were harvested from 24-h culture fluids of virus-infected confluent cells for complementary DNA (cDNA) synthesis and from 6-to 8-h culture fluids for polyadenylic acid [poly(A)]-containing virus RNA. Virus was purified from culture fluids as described previously (26,29). The FeLV (Gardner-Arnstein strain) used in this study represents a mixture of subgroups A and B.…”

Section: Methodsmentioning

confidence: 99%