Deoxyribonucleic Acid Synthesis Time of Erythropoietic and Granulopoietic Cells in Human Beings

Stryckmans, Pierre; Ep, Cronkite; Fache, J.; Fliedner, Theodor M.; Ramos, J L A

doi:10.1038/211717a0

Cited by 98 publications

(30 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This is in keeping with previous studies of patients with acute leukemia in whom the percentages of marrow blasts initially labeled with thymidine-'H were as follows: 6.1% (0.2 to 9.1%o) (10), 7.9%o (2.2 to 26.5%o) (48), 9.9%o (3.0 to 24%o) (49), and 9%o (4.1 to 17.3%o) (13). The initial labeling indexes of our patients' circulating blasts were even lower than in the marrow, again in keeping with most other studies in which both leukemic marrow and blood were studied (9,13,(49)(50)(51).…”

Section: Percentage Of Blasts Synthesizing Dnasupporting

confidence: 70%

“…The only comparable measurements of the duration of DNA synthesis in normal granulocyte precursors are in two patients in whom it was estimated to be 13 to 14 hours (7,8). Ota (56) has studied the appearance times of labeled blast mitoses in vivo in three additional patients with acute leukemia, and in all three the duration of S was roughly 20 hours.…”

Section: Duration Of Phases Of Mitotic Cyclementioning

confidence: 99%

“…Many die but many others persist in the marrow and elsewhere as primitive cells and continue to proliferate. As the blasts accumulate, they Introduction Although there have been recent advances in knowledge about normal hematopoiesis (1)(2)(3)(4)(5)(6)(7)(8), information is still limited about cellular proliferation in acute leukemia (8)(9)(10)(11)(12)(13)(14). The present report is a study of two adults with acute leukemia in whom in vivo measurements were made of the mean generation times of their leukemic cells and of the phases of their mitotic cycles.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Studies of Cellular Proliferation in Human Leukemia. I. Estimation of Growth Rates of Leukemic and Normal Hematopoietic Cells in Two Adults with Acute Leukemia Given Single Injections of Tritiated Thymidine *

Clarkson¹,

Ohkita²,

K³

et al. 1967

J. Clin. Invest.

163

View full text Add to dashboard Cite

Summary. Two adults with rapidly progressive acute myeloblastic and myelomonoblastic leukemia were given single injections of tritiated thymidine, and measurements were made of the growth rates of their leukemic and normal hematopoietic cells by radioautographic methods. Although almost all leukemic blasts in both marrow and blood were metabolically active as shown by their ability to incorporate tritiated uridine and leucine in Vitro, only 5.6% and 6.1% ol the blasts in the marrow and even fewer in the blood incorporated tritiated thymidine. The mitotic indexes of the marrow blasts were 0.66% and 0.52%; no circulating blasts were dividing. The mean generation times of the actively proliferating blasts were estimated to be 49 and 83 hours. This cannot be equated with the doubling time of the total leukemic population as there is evidence that many blasts fail to continue dividing and die. The mean durations of the phases of the blasts' mitotic cycles were as follows: DNA synthesis (S) = 22 and 19 hours, premitosis (G2) = 3 hours, mitosis (M) = 0.47 and 0.62 hour (minimal estimates), and postmitosis (G1) = 24 and 61 hours. In both patients the maximal mean transit time of the blasts in the blood was 36 hours, and the minimal numbers of actively dividing blasts present were 1.6 and 2.6 X 109 per kg of body weight.Estimates were also made of the rates of proliferation and maturation of the residual normal erythrocytic and granulocytic cells in these two patients. Although total production was markedly diminished because of reduction in the number of normal elements, the relatively few remaining normal cells appeared to be dividing and maturing at rates that are about the same or only slightly slower than those found in normal subjects.We conclude that the main reason leukemic blasts displace normal hematopoietic precursors in acute leukemia is that the blasts largely fail to differentiate. Many die but many others persist in the marrow and elsewhere as primitive cells and continue to proliferate. As the blasts accumulate, they

show abstract

Section: Percentage Of Blasts Synthesizing Dnasupporting

confidence: 70%

Section: Duration Of Phases Of Mitotic Cyclementioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Studies of Cellular Proliferation in Human Leukemia. I. Estimation of Growth Rates of Leukemic and Normal Hematopoietic Cells in Two Adults with Acute Leukemia Given Single Injections of Tritiated Thymidine *

Clarkson¹,

Ohkita²,

K³

et al. 1967

J. Clin. Invest.

163

View full text Add to dashboard Cite

show abstract

“…Since the growth fraction varies in different tissues, and particularly among neoplasms, such estimates have been inaccurate. Recently, several normal patients and patients with leukemia, neoplastic effusions and solid tumors have been studied using serial sample techniques after 3HTdR-labeling in vivo (Bennington, 1969;Clarkson et al, 1965;Lala et al, 1965;Lipkin, Bell and Sherlock, 1963;Lipkin, Sherlock and Bell, 1963;Mauer andFisher, 1963, 1966;Stryckmans et al, 1966 Lala (1968) applied the analysis to the Ehrlich ascites tumor in mice, where the growth fraction declines with increasing tumor age due to transition from a dividing to a nondividing state occurring mostly at the end of mitosis (Lala et al, 1965).…”

Section: Fabrikantmentioning

confidence: 99%

The Kinetic of Cellular Proliferation in Human Tissues. Determination of Duration of DNA Synthesis Using Double Labeling Autoradiography

Fabrikant¹

1970

Br J Cancer

View full text Add to dashboard Cite

SUMMARY.-The duration of the DNA synthesis period in normal and malignant human tissues from the larynx, trachea bronchus and esophagus of 13 patients has been estimated by a tritiated thymidine, carbon-14 thymidine double-labeling method in vitro. The DNA synthesis times range from approximately 10 to 25 hours, are shortest in normal tissues, and are longer in malignant tumor cells than in benign tumor cells. The double-labeling method, the analysis of thymidine labeling index data for the measurement of the S period, and a model for determining potential doubling times in human tissues are described and discussed.

show abstract

“…This time in normal man was reported to be about 13 to l4 hours (Stryckmans et , 1966) and sim ilar values were found in human chronic myeloid leukemia cells (Vincent et al,I969). Maloney et al (1962) reported the S stage for canine myelocytes to be about 7 hours.…”

Section: Kinetics Of Granulopoiesis Cell Population Kineticsmentioning

confidence: 99%

Granulocyte kinetics in cats

Prasse

View full text Add to dashboard Cite

Deoxyribonucleic Acid Synthesis Time of Erythropoietic and Granulopoietic Cells in Human Beings

Cited by 98 publications

References 6 publications

Studies of Cellular Proliferation in Human Leukemia. I. Estimation of Growth Rates of Leukemic and Normal Hematopoietic Cells in Two Adults with Acute Leukemia Given Single Injections of Tritiated Thymidine *

Studies of Cellular Proliferation in Human Leukemia. I. Estimation of Growth Rates of Leukemic and Normal Hematopoietic Cells in Two Adults with Acute Leukemia Given Single Injections of Tritiated Thymidine *

The Kinetic of Cellular Proliferation in Human Tissues. Determination of Duration of DNA Synthesis Using Double Labeling Autoradiography

Granulocyte kinetics in cats

Contact Info

Product

Resources

About