Deoxyribonucleoprotein Changes in Ram and Bull Spermatids

Loir, Maurice; Reviers, M.T. Hochereau de

doi:10.1530/jrf.0.0310127

Cited by 13 publications

(15 citation statements)

References 10 publications

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“…1, Figs 2 and 4). Our results, which indicate that AMD-binding decreases as cells progress in their development from primary spermatocytes to round spermatids, are in agreement with the data of Brächet & Hulin (1969) and Loir & Hochereau-de Reviers (1972). Since relatively large amounts of RNA are synthesized in Chinese hamster leptotene and pachytene nuclei (Utakoji, 1966), and treatment with AMD inhibits this RNA synthesis (Barcellona & Brinkley, 1973), it is possible that the physicochemical status of chromatin material in these cells influences their AMD-binding capacities.…”

Section: Resultssupporting

confidence: 89%

“…Brächet & Hulin (1969) have shown that mouse spermatocytes bind more AMD than spermatids or spermatozoa, but neither specific types of spermatocytes nor specific spermatid steps were identified. Loir & Hochereau-de Reviers (1972) found that pachytene spermatocytes were more heavily labelled with AMD than were round sperma¬ tids in the bull. We have demonstrated that leptotene and pachytene sperma¬ tocytes bound more AMD than corresponding round spermatids in the same respective seminiferous tubules from Chinese hamsters (Table 2 and PI.…”

Section: Resultsmentioning

confidence: 93%

“…The second aspect of the present work localizes and estimates the extent of differential binding of [3H]AMD in vivo to some spermatogenic cells of the Chinese hamster. Actinomycin D has been shown to bind selectively with different types of somatic cells (Bolund, 1970), with different cells from the same population (Bolund, 1970;Ringertz, Darzynkiewicz & Bolund, 1969), with synchronously dividing tissue culture cells during the cell cycle (Pederson & Robbins, 1972) and with different types of spermatogenic cells in the mouse (Brächet & Hulin, 1969), ram (Loir & Hochereau-de Reviers, 1972) and bull Loir & Hochereau-de Reviers, 1972). All of the studies on spermatogenic cells have employed quantitative autoradiography of whole-cell preparations that were incubated with [3H]AMD in vitro.…”

Section: Resultsmentioning

confidence: 99%

“…There have been reports in the literature concerning the binding of [3H] AMD to mouse (Brächet & Hulin, 1969), bull (Darzynkiewicz, Gledhill & Ringertz, 1969;Loir & Hochereau-de Reviers, 1972) and ram (Loir & Hochereau-de Reviers, 1972) spermatogenic cells using quantitative autoradiography. Mouse and ram cells were exposed to labelled antibiotic after fixation, and cells from the bull were cultured with [3H]AMD in vitro before fixation.…”

Section: Introductionmentioning

confidence: 99%

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Differential Binding of Tritiated Actinomycin to the Nuclei of Mammalian Spermatogenic Cells in Vivo

Barcellona¹,

Brackeen²,

Brinkley³

1974

Reproduction

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Chinese hamsters, Cricetulus griseus, were given a single injection of 2\m=.\0 \g=m\g[3H]actinomycin D directly into the left testis. For 24 hr after injection, the distribution of the radioisotope in the reproductive organs, blood plasma and liver was followed, using liquid scintillation counting techniques. After 24 hr, nearly 50% of the injected dose remained in the left testis while insignificant amounts of label were associated with the right testis. The left epididymis gradually accumulated radioisotope probably by intratubular transport from the left testis. The right epididymis possessed negligible amounts of label after 24 hr. Levels of the antibiotic in blood plasma and liver steadily decreased during the experimental period.Localization of [3H]actinomycin D within the spermatogenic cells of the seminiferous epithelium was determined by autoradiography. The results indicated that the antibiotic was differentially bound by various types of spermatogenic cells. Of the spermatocytes, Stage VIII leptotene nuclei (31\m=.\6grains/nucleus) were much more heavily labelled compared to early Stage V pachytene nuclei (6\m=.\2grains/nucleus). Actinomycin D was lightly bound to nuclei of Step 5 and Step 8 round spermatids (3\m=.\6 and 3\m=.\8 grains/nucleus, respectively). Elongating spermatid nuclei (Steps 9 to 14) were lightly labelled at best, whereas elongated spermatid nuclei (Steps 15 to 18) were very heavily grained. The label intensity decreased in Step-19 spermatids (spermiation step). Labelling variations among spermatogenic cells may be attributable to the physicochemical state of deoxyribonucleoprotein complexes in these cells.

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Section: Resultssupporting

confidence: 89%

Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Differential Binding of Tritiated Actinomycin to the Nuclei of Mammalian Spermatogenic Cells in Vivo

Barcellona¹,

Brackeen²,

Brinkley³

1974

Reproduction

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“…The DNA-protamine complex shows a degree of condensation similar to that found in the bacteriophage head and possesses low capacity for binding of actinomycin D and cationic dyes (Olins et al, 1968;Darzynkiewicz et al, 1969;Loir & Hochereau de Reviers, 1972;Mezquita Vol. 170 & Teng, 1977).…”

mentioning

confidence: 91%

Studies on sex-organ development. Changes in chromatin structure during spermatogenesis in maturing rooster testis as demonstrated by the initiation pattern of ribonucleic acid synthesis in vitro

Mezquita¹,

Teng²

1978

Biochemical Journal

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To probe the structural change in the genome of the differentiating germ cell of the maturing rooster testis, the chromatin from nuclei at various stages of differentiation were transcribed with prokaryotic RNA polymerase from Escherichia coli or with eukaryotic RNA polymerase II from wheat germ. The transcription was performed under conditions of blockage of RNA chain reinitiation in vitro with rifampicin or rifamycin AF/013. With the E. coli enzyme, the changes in (1) the titration curve for the enzyme-chromatin interaction, (2) the number ofinitiation sites, (3) the rate ofelongation ofRNA chains, and (4) the kinetics of the formation of stable initiation complexes revealed the unmasking of DNA in elongated spermatids and the masking of DNA in spermatozoa. In both cases the stability of the DNA duplex in the initiation region for RNA synthesis greatly increased. In contrast with the E. coli enzyme, the wheat-germ RNA polymerase II was relatively inefficient at transcribing chromatin of elongated spermatids. Such behaviour can be predicted if unmasked double-stranded DNA is present in elongated spermatids.

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Separation of ram spermatids by sedimentation at unit gravity

Loir

Lanneau

1974

Experimental Cell Research

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Deoxyribonucleoprotein Changes in Ram and Bull Spermatids

Cited by 13 publications

References 10 publications

Differential Binding of Tritiated Actinomycin to the Nuclei of Mammalian Spermatogenic Cells in Vivo

Differential Binding of Tritiated Actinomycin to the Nuclei of Mammalian Spermatogenic Cells in Vivo

Studies on sex-organ development. Changes in chromatin structure during spermatogenesis in maturing rooster testis as demonstrated by the initiation pattern of ribonucleic acid synthesis in vitro

Separation of ram spermatids by sedimentation at unit gravity

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