Deoxyshikonin inhibited rotavirus replication by regulating autophagy and oxidative stress through SIRT1/FoxO1/Rab7 axis

Huang, Haohai; Liú, Dan; He, Bin; Pu, Rong; Cui, Yejia; Zhou, Guanghui

doi:10.1016/j.micpath.2023.106065

Cited by 4 publications

(3 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Increase in cellular ROS and a decreased intracellular GSH concentration has also been reported in RV-SA11-infected Caco-2 cells ( Buccigrossi et al., 2014 ; Cui et al., 2018 ; Guerrero et al., 2016 ; Huang et al., 2023 ; Paparo et al., 2020 ). A study done in RV infected mice model also showed reduced GSH levels in RV-infected mice compared to that of mock-infected mice ( Katyal et al., 1999 ).…”

Section: Discussionmentioning

confidence: 53%

Rotavirus-induced lncRNA SLC7A11-AS1 promotes ferroptosis by targeting cystine/glutamate antiporter xCT (SLC7A11) to facilitate virus infection

Banerjee,

Sarkar,

Mukherjee

et al. 2024

Virus Research

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 53%

Rotavirus-induced lncRNA SLC7A11-AS1 promotes ferroptosis by targeting cystine/glutamate antiporter xCT (SLC7A11) to facilitate virus infection

Banerjee,

Sarkar,

Mukherjee

et al. 2024

Virus Research

View full text Add to dashboard Cite

“…32 These studies demonstrate that shikonin and its natural or synthetic derivatives possess a spectrum of benefits in immune disorders and malignancies. DSK is a natural derivative of shikonin, as the other shikonin derivatives, has a variety of inhibitory effects on cytochrome P450 activity, 33 cancer cell proliferation, 13 and rotavirus replication 34 via multiple targets and signaling pathways. In this study, we further investigate the anticancer effect of DSK on cervical cancer cells, and our results reveal that DSK significantly reduces the viability of HeLa and SiHa cells associated with induction of apoptosis and downregulation of cIAP-1, F I G U R E 5 DSK triggers apoptotic signaling in human cervical cancer cells.…”

Section: Discussionmentioning

confidence: 99%

Deoxyshikonin triggers apoptosis in cervical cancer cells through p38 MAPK‐mediated caspase activation

Lee,

Chen,

Kao

et al. 2024

Environmental Toxicology

View full text Add to dashboard Cite

Deoxyshikonin (DSK) is a biological component derived from Lithospermum erythrorhizon. Although DSK possesses potential anticancer activities, whether DSK exerts anticancer effects on cervical cancer cells is incompletely explored. This study was aimed to investigate the anticancer activity of DSK against cervical cancer cells and its molecular mechanisms. Cell viability was evaluated by MTT assay. Level of phosphorylation and protein was determined using Western blot. Involvement of signaling kinases was assessed by specific inhibitors. Our results revealed that DSK reduced viability of human cervical cell in a dose‐dependent fashion. Meanwhile, DSK significantly elicited apoptosis of HeLa and SiHa cells. Apoptosis microarray was used to elucidate the involved pathways, and the results showed that DSK dose‐dependently diminished cellular inhibitor of apoptosis protein 1 (cIAP1), cIAP2, and XIAP, and induced cleavage of poly(ADP‐ribose) polymerase (PARP) and caspase‐8/9/3. Furthermore, we observed that DSK significantly triggered activation of ERK, JNK, and p38 MAPK (p38), and only inhibition of p38 diminished the DSK‐mediated pro‐caspases cleavage. Taken together, our results demonstrate that DSK has anti‐cervical cancer effects via the apoptotic cascade elicited by downregulation of IAPs and p38‐mediated caspase activation. This suggests that DSK could act as an adjuvant to facilitate cervical cancer management.

show abstract

“…Genipin, isolated from jasmine flowers, inhibits human rotavirus Wa strain and simian rotavirus SA-11 strain in vitro by inhibiting two different stages of the viral replication cycle: attachment and penetration (early stage) in pre-treatment and assembly and release (late stage) in post-treatment [ 6 ]. Deoxyshikonin can inhibit rotavirus replication by inducing low SIRT1, ac-Foxo1, Rab7, and VP6 protein levels, low RV titers, low autophagy, and oxidative stress [ 7 ]. The antiviral effect of Portulaca oleracea L. polysaccharide (POL-P), an active component of Portulaca oleracea L(POL), inhibits rotavirus replication by upregulating the expression of IFN-α [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

ML241 Antagonizes ERK 1/2 Activation and Inhibits Rotavirus Proliferation

Wang,

Hu,

et al. 2024

Viruses

View full text Add to dashboard Cite

Rotavirus (RV) is the main pathogen that causes severe diarrhea in infants and children under 5 years of age. No specific antiviral therapies or licensed anti-rotavirus drugs are available. It is crucial to develop effective and low-toxicity anti-rotavirus small-molecule drugs that act on novel host targets. In this study, a new anti-rotavirus compound was selected by ELISA, and cell activity was detected from 453 small-molecule compounds. The anti-RV effects and underlying mechanisms of the screened compounds were explored. In vitro experimental results showed that the small-molecule compound ML241 has a good effect on inhibiting rotavirus proliferation and has low cytotoxicity during the virus adsorption, cell entry, and replication stages. In addition to its in vitro effects, ML241 also exerted anti-RV effects in a suckling mouse model. Transcriptome sequencing was performed after adding ML241 to cells infected with RV. The results showed that ML241 inhibited the phosphorylation of ERK1/2 in the MAPK signaling pathway, thereby inhibiting IκBα, activating the NF-κB signaling pathway, and playing an anti-RV role. These results provide an experimental basis for specific anti-RV small-molecule compounds or compound combinations, which is beneficial for the development of anti-RV drugs.

show abstract

Deoxyshikonin inhibited rotavirus replication by regulating autophagy and oxidative stress through SIRT1/FoxO1/Rab7 axis

Cited by 4 publications

References 44 publications

Rotavirus-induced lncRNA SLC7A11-AS1 promotes ferroptosis by targeting cystine/glutamate antiporter xCT (SLC7A11) to facilitate virus infection

Rotavirus-induced lncRNA SLC7A11-AS1 promotes ferroptosis by targeting cystine/glutamate antiporter xCT (SLC7A11) to facilitate virus infection

Deoxyshikonin triggers apoptosis in cervical cancer cells through p38 MAPK‐mediated caspase activation

ML241 Antagonizes ERK 1/2 Activation and Inhibits Rotavirus Proliferation

Contact Info

Product

Resources

About