Dependence of alignment direction on magnitude of strain in esophageal smooth muscle cells

Ritchie, Alastair Campbell; Wijaya, Surya Maitri; Ong, Wey Feng; Zhong, Shan; Chian, Kerm Sin

doi:10.1002/bit.22190

Cited by 30 publications

(27 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Uniaxial cyclic stretching of 2D cell cultures causes the actin cytoskeleton and focal adhesions of many cell types to align perpendicular to the direction of stretch [15,16,17]. Different cell types orient at different rates, for instance, fibroblasts orient within 3 hours [24] and vascular endothelial cells orient within 6 hours [25], while some cell types, including neutrophils, do not reorient [26].…”

Section: Discussionmentioning

confidence: 99%

“…AF strains calculated from MRI support the presence of large axial, radial and shear strains under physiological loading and further demonstrate strain amplification during injury and degeneration [5,7]. High magnitudes of cyclic equibiaxial strain induced apoptosis at 15% stretch in rabbit AF cells [12] and at 20% stretch in rat AF cells [13,14], however equibiaxial strain suppresses reorientation effects observed in pure uniaxial stretch of other cell types [15,16,17]. The hypothesis of this study was that human degenerated AF cells obtained from surgical samples reorient perpendicular to excessive uniaxial strains (10%, 15%, and 20% stretch), as observed in other cell types, but will undergo apoptosis when their adaptive reorientation is restricted as might occur around defects or crosslinking found with degeneration.…”

Section: Introductionmentioning

confidence: 97%

See 1 more Smart Citation

Live free or die: Stretch-induced apoptosis occurs when adaptive reorientation of annulus fibrosus cells is restricted

Abbott

Howe

Langevin

et al. 2012

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

High matrix strains in the intervertebral disc occur during physiological motions and are amplified around structural defects in the annulus fibrosus (AF). It remains unknown if large matrix strains in the human AF result in localized cell death. This study investigated strain amplitudes and substrate conditions where AF cells were vulnerable to stretch-induced apoptosis. Human degenerated AF cells were subjected to 1Hz-cyclic tensile strains for 24 hours on uniformly collagen coated substrates and on substrates with 40 μm stripes of collagen that restricted cellular reorientation. AF cells were capable of responding to stretch (stress fibers and focal adhesions aligned perpendicular to the direction of stretch), but were vulnerable to stretch-induced apoptosis when cytoskeletal reorientation was restricted, as could occur in degenerated states due to fibrosis and crosslink accumulation and at areas where high strains occur (around structural defects, delaminations, and herniations).

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Live free or die: Stretch-induced apoptosis occurs when adaptive reorientation of annulus fibrosus cells is restricted

Abbott

Howe

Langevin

et al. 2012

Biochemical and Biophysical Research Communications

View full text Add to dashboard Cite

show abstract

“…Therefore, mimicking the native cell alignment is necessary in engineered muscle tissue (smooth, skeletal, and cardiac) to achieve effective contraction. 10 On the other hand, vascular cells are arranged in distinct patterns in the multiple layers of the arterial wall with circumferentially aligned vascular SMCs in the media and longitudinally aligned endothelial cells in the intima. 11 In the context of wound repair, axially aligned fibroblasts within the healing tissues (guided tissue regeneration) are desirable.…”

Section: Introductionmentioning

confidence: 99%

Automated and Adaptable Quantification of Cellular Alignment from Microscopic Images for Tissue Engineering Applications

Beyazoglu

Hefner

et al. 2011

Tissue Engineering Part C: Methods

View full text Add to dashboard Cite

Cellular alignment plays a critical role in functional, physical, and biological characteristics of many tissue types, such as muscle, tendon, nerve, and cornea. Current efforts toward regeneration of these tissues include replicating the cellular microenvironment by developing biomaterials that facilitate cellular alignment. To assess the functional effectiveness of the engineered microenvironments, one essential criterion is quantification of cellular alignment. Therefore, there is a need for rapid, accurate, and adaptable methodologies to quantify cellular alignment for tissue engineering applications. To address this need, we developed an automated method, binarization-based extraction of alignment score (BEAS), to determine cell orientation distribution in a wide variety of microscopic images. This method combines a sequenced application of median and band-pass filters, locally adaptive thresholding approaches and image processing techniques. Cellular alignment score is obtained by applying a robust scoring algorithm to the orientation distribution. We validated the BEAS method by comparing the results with the existing approaches reported in literature (i.e., manual, radial fast Fourier transform-radial sum, and gradient based approaches). Validation results indicated that the BEAS method resulted in statistically comparable alignment scores with the manual method (coefficient of determination R 2 = 0.92). Therefore, the BEAS method introduced in this study could enable accurate, convenient, and adaptable evaluation of engineered tissue constructs and biomaterials in terms of cellular alignment and organization.

show abstract

“…Alignment of tissue cells plays a key role in various important functions of the tissues such as neuron regeneration, defining mechanical and physical properties of tissue, pattern formation in embryogenesis, tissue maturation, etc. [14][15][16][17][18][19][20][21][22] . Further, given the increasing use of regenerative medicines and tissue implant, the understanding of tissue functioning has become very important.…”

Section: Importance Of Cell Alignment and Fibre Alignment In Biologymentioning

confidence: 99%

Extracting Cell and Fibre Orientation from Microscopic Images:Computational Methods and Tools

Sharma

2016

Current Science

View full text Add to dashboard Cite

Orientation of cell and its actin fibres dictate various properties of the cell and its associated tissue including morphology, cell migration and response of cell to external stimuli. While the vital role of cell and actin fibre orientation has been demonstrated in various studies and across many cell-types, extracting this information from microscopy images is a challenging task. Although several methods have been developed to quantify alignment of a cell and its actin fibres from experimental images, all of them differ at one step or the other. We highlight the importance of orientation of cell and associated fibres and present a generic scheme of various approaches to assimilate the widely used methods. We have presented a systematic approach to determine cell and fibre orientation. The study will improve our understanding of the core processes and will also help in development of high throughput imaging methods to extract information from experimental images.Keywords: BEAS, cell orientation, DFT, fibre orientation, FFT, image processing, thresholding.IN the study of biological phenomena, cell is at a very fundamental level, as it is the smallest building block of all organisms. In fact, it is the smallest form of life that exhibits all the basic traits that form life such as birth, ingestion, digestion, protection and finally death. Cell during its life cycle experiences a number of mechanical interactions with surrounding environment necessary for its communication, proliferation, differentiation and migration [1][2][3][4][5][6][7][8] . A cell responds to these mechanical forces by exhibiting changes at various levels such as in morphology, protein synthesis 9,10 , gene expression 11,12 , etc. Then, cell tries to adapt to its environment through these changes. Several of its mechanical properties facilitate the adaptation of cell to the associated environment. Therefore, to understand the mechanism of working of a cell, knowledge of its mechanical properties and how do these get affected by a cell's surrounding environment is very vital. Microscopy-based imaging and its subsequent analysis is one of the traditional and widely used approaches to gain insights into the cell's mechanical and morphological properties. While microscopy imaging is a widely used approach to extract quantitative information about cell morphology, image analysis and morphology quantification are still challenging. One of the morphological features of a cell, which has been shown to exhibit several genotypic and phenotypic properties of a cell is the orientation of the cell. Similarly, orientation of fibres has been shown to exhibit various properties of associated cell population, including their density, topography, etc.The orientation of cell and the associated fibres are the indicative of many physiological and disease conditions. While microscopy-based image acquisition is the traditional method to gain insights into the orientation and morphology of cell and associated fibres, extracting thequantitative information about the c...

show abstract

Dependence of alignment direction on magnitude of strain in esophageal smooth muscle cells

Cited by 30 publications

References 41 publications

Live free or die: Stretch-induced apoptosis occurs when adaptive reorientation of annulus fibrosus cells is restricted

Live free or die: Stretch-induced apoptosis occurs when adaptive reorientation of annulus fibrosus cells is restricted

Automated and Adaptable Quantification of Cellular Alignment from Microscopic Images for Tissue Engineering Applications

Extracting Cell and Fibre Orientation from Microscopic Images:Computational Methods and Tools

Contact Info

Product

Resources

About