Dependence of Complex Formation of (1 → 3)-<i>β</i>-<scp>d</scp>-Glucan with Congo Red on Temperature in Alkaline Solutions

Ogawa, K.; Dohmaru, Takaaki; Yui, Toshifumi

doi:10.1271/bbb.58.1870

Cited by 32 publications

(17 citation statements)

References 4 publications

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“…Changes in the surrounding conditions (e.g., due to interactions with other molecules) determine a detectable shift in the maximum of the CR absorption spectrum. More interestingly, CR may show an induced CD signal if the interaction takes place with ordered macromolecules [25,26]. We have used these CR properties to follow the triplex denaturation in the unperturbed Sclg and after sonication.…”

Section: Uv and CD Spectra Of Congo Red In The Presence Of Scleroglucanmentioning

confidence: 99%

“…It is known that the interaction between Sclg triplex and Congo red (CR) produces a shift in the CR absorption band and, more interestingly, induces a circular dichroism (CD) signal [22][23][24][25][26]. This signal is lost when triplex unfolds, suggesting that after denaturation Sclg loses the triplex configuration, but the structural details on the Sclg/CR complexes are still missing.…”

Section: Introductionmentioning

confidence: 99%

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Relative Stability of the Scleroglucan Triple-Helix and Single Strand: an Insight from Computational and Experimental Techniques

Bocchinfuso

Meisina

Conflitti

et al. 2016

Zeitschrift Für Physikalische Chemie

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Scleroglucan (Sclg) is a polysaccharide that exhibits a triple helix conformation (triplex), both in aqueous solution and in the solid state, which is lost in DMSO solution, at high temperature and at high pH values. The triplex conformation is characterized by a high rigidity, responsible of Sclg peculiar properties. Although the relative stability of triplex and single strand has already been investigated, different structural details are still missing. In the present study, we analyse the structural properties and the factors stabilizing the single chain and the triple helix of Sclg in different conditions. To this end, we simulated both systems in water and in DMSO. The triple helix has been also simulated in the presence of chemical damages on one of the three strands (to reproduce in silico the effect of sonication) or by inducing a partial unfolding of the triplex structure. The computational results have been compared with experimental evidences in which the triplex denaturation, at alkaline pH values, has been followed by monitoring the UV and CD spectra of Congo red, used as a probe molecule. Our results indicate that sonication breaks the Sclg chains without appreciably changing the stability of the other tracts of triple helix. The simulated perturbed or partially unfolded triplexes show a clear tendency to form less Unauthenticated Download Date | 6/23/16 1:58 PM 2 | G. Bocchinfuso et al. ordered aggregates. Finally, our simulations put in evidence an important role of the hydrophobic interactions both in the triplex stability and in the aggregation processes observed after induced denaturation.

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Section: Uv and CD Spectra Of Congo Red In The Presence Of Scleroglucanmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Relative Stability of the Scleroglucan Triple-Helix and Single Strand: an Insight from Computational and Experimental Techniques

Bocchinfuso

Meisina

Conflitti

et al. 2016

Zeitschrift Für Physikalische Chemie

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“…Therefore, we determined the cell wall structure and major compounds of G. lucidum to investigate the role of ugp in cell wall formation. We used the cell wall stress compounds Congo Red (CR, inhibits b-1,3-glucan assembly in the cell wall (Ogawa et al, 1994)), Calcofluor White (CFW, inhibits the assembly of chitin chains in the wall (Yoshimi et al, 2013)) and SDS (perturbs membrane integrity (Munro et al, 2007)) to determine the sensitivity of the ugpi strains to cell wall stress. The results showed that the ugpi strains were more tolerant of CFW (Figs.…”

Section: The Cell Wall Structure Was Altered In the Ugpi Mutantsmentioning

confidence: 99%

UDP-glucose pyrophosphorylase influences polysaccharide synthesis, cell wall components, and hyphal branching in Ganoderma lucidum via regulation of the balance between glucose-1-phosphate and UDP-glucose

Chen

Gao

et al. 2015

Fungal Genetics and Biology

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“…Polysaccharides showing a triple helix conformation form a complex with Congo red in alkaline solutions, which is stabilized by strong hydrogen bonds and/or hydrophobic interactions between the polysaccharide and the dye molecule. Complexation is characterized by the red shift of the absorption maximum of the Congo red solutions (489 nm) to longer wavelengths up to 520 nm [45].…”

Section: Conformational Arrangementmentioning

confidence: 99%

Structural Features and Healthy Properties of Polysaccharides Occurring in Mushrooms

2012

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Abstract:Polysaccharides from mushrooms have attracted a great deal of attention due to the many healthy benefits they have demonstrated, such as immunomodulation, anticancer activity, prevention and treatment of cardiovascular diseases, antiviral and antimicrobial effects, among others. Isolation and purification of polysaccharides commonly involve several steps, and different techniques are actually available in order to increase extraction yield and purity. Studies have demonstrated that the molecular structure and arrangement significantly influence the biological activity; therefore, there is a wide range of analytical techniques for the elucidation of chemical structures. Different polysaccharides have been isolated from mushrooms, most of them consisting of β-linked glucans, such as lentinan from Lentinus edodes, pleuran from Pleurotus species, schizophyllan from Schizophyllum commune, calocyban from Calocybe indica, or ganoderan and ganopoly from Ganoderma lucidum. This article reviews the main methods of polysaccharide isolation and structural characterization, as well as some of the most important polysaccharides isolated from mushrooms and the healthy benefits they provide.

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Dependence of Complex Formation of (1 → 3)-β-d-Glucan with Congo Red on Temperature in Alkaline Solutions

Cited by 32 publications

References 4 publications

Relative Stability of the Scleroglucan Triple-Helix and Single Strand: an Insight from Computational and Experimental Techniques

Relative Stability of the Scleroglucan Triple-Helix and Single Strand: an Insight from Computational and Experimental Techniques

UDP-glucose pyrophosphorylase influences polysaccharide synthesis, cell wall components, and hyphal branching in Ganoderma lucidum via regulation of the balance between glucose-1-phosphate and UDP-glucose

Structural Features and Healthy Properties of Polysaccharides Occurring in Mushrooms

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