Dependence of Energy Metabolism on the Density of Cells in Culture.

Bereiter‐Hahn, Jürgen; Münnich, A.; Woiteneck, P.

doi:10.1247/csf.23.85

Cited by 45 publications

(40 citation statements)

References 23 publications

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“…Cell density has been shown to affect the energy metabolism [72], activation of p53 pathway [73] and de novo synthesis of sphingolipids [74]. Additionally viral productivity depends on other factors, including cell substrate, cell cycle, culture time at the infection, multiplicity of infection, culture medium and feeding mode [61,75-77].…”

Section: Feeding Strategies and Medium Modification For Viral Productmentioning

confidence: 99%

Influence of HEK293 metabolism on the production of viral vectors and vaccine

Petiot

Čuperlović-Culf

Shen

et al. 2015

Vaccine

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Section: Feeding Strategies and Medium Modification For Viral Productmentioning

confidence: 99%

Influence of HEK293 metabolism on the production of viral vectors and vaccine

Petiot

Čuperlović-Culf

Shen

et al. 2015

Vaccine

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“…The NAD ϩ /NADH and NADP ϩ /NADPH couples have received less attention regarding their role in cell proliferation (7,36). NAD works as a carrier, taking electrons from catabolic reactions and redirecting them into the oxidative phosphorylation chain, enabling cells to maximize energy production from catabolic metabolism (34).…”

Section: Attene-ramos Matias S Kajorn Kitiphongspattana Katrinmentioning

confidence: 99%

“…NOmediated signaling is also involved in both induction (14, 59) and inhibition (2, 16) of cell proliferation as well as the induction of genotoxicity, mitochondrial damage, and apoptosis (30). These differential effects seem to be concentration dependent (13).The NAD ϩ /NADH and NADP ϩ /NADPH couples have received less attention regarding their role in cell proliferation (7,36). NAD works as a carrier, taking electrons from catabolic reactions and redirecting them into the oxidative phosphorylation chain, enabling cells to maximize energy production from catabolic metabolism (34).…”

mentioning

confidence: 99%

Temporal changes of multiple redox couples from proliferation to growth arrest in IEC-6 intestinal epithelial cells

Attene‐Ramos¹,

Kitiphongspattana²,

Ishii-Schrade³

et al. 2005

American Journal of Physiology-Cell Physiology

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Changes in intracellular redox couples and redox reactive molecules have been implicated in the regulation of a variety of cellular processes, including cell proliferation and growth arrest by contact inhibition. However, the magnitude, direction, and temporal relationship of redox changes to cellular responses are incompletely defined. The present work sought to characterize redox and metabolic changes associated with proliferative stages to contact inhibition of growth in rat IEC-6 intestinal epithelial cells. From the first day of culture until 1 day before confluence, an increase in GSH concentrations and a significant reduction in the redox potential of the GSSG/2GSH couple were observed. These changes were accompanied by a decrease in relative reactive oxygen species (ROS) and nitric oxide (NO) concentrations and oxidation of the redox potential of the NADP(+)/reduced NADP and NAD(+)/NADH couples. Postconfluent cells exhibited a significant decrease in GSH concentrations and a significant oxidation of the GSSG/2GSH couple. When cell proliferation decreased, relative ROS concentrations increased (P < 0.01), whereas NO concentrations remained unchanged, and the NAD(+)/NADH couple became more reduced. Together, these data indicate that the redox potential of distinct couples varies differentially in both magnitude and direction during successive stages of IEC-6 growth. This finding points out the difficulty of defining intracellular redox status at particular stages of cell growth by examining only one redox species. In addition, the data provide a numerical framework for future research of regulatory mechanisms governed by distinct intracellular redox couples.

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“…For instance, it has been shown that cell density influences energy metabolism (Bereiter-Hahn et al 1998), the activation of p53 tumor suppressor (Bar et al 2004), the de novo synthesis of sphingolipids (Vukelic and KalanjBognar 2001) and sensitivity to drugs (Masquelier and Vitols 2004), and recently the ''critical cell density'' of a cultured population was thought to be involved in the adaptive drug resistant mechanism (de Anta et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Heterogeneity in tumor cell energetic metabolome at different cell cycle phases of human colon cancer cell lines

Maddula

Baumbach

2010

Metabolomics

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In this present study, the efficacy of metabolomics as a tool for tumor cell energetics for in vitro cell cultures was demonstrated with full competence for the first time by elucidating the anabolic and energy-yielding segments of glycolysis and glutaminolysis, which constitute a part of energy metabolism in tumor cells. By synchronizing colon cancer cells SW480 and SW620 in culture, the metabolome specific to cell cycle phases was analyzed using nuclear magnetic resonance spectroscopy. At the G 1 /S transition of the cell cycle (i.e. transition from cell growth to duplication of genetic material), the majority of the energy production was realized by glycolysis through a high channeling of glucose carbons towards lactate. During the late S phase, the majority of energy was produced by glutaminolysis through a high channeling of glutamine carbons towards lactate, while the glucose carbons were channeled towards bio-synthetic pathways. These results indicate that the metabolism of proliferating cells is heterogeneous throughout the cell cycle and can be better interpreted on the basis of different cell cycle phases. These findings could be exploited for the development of a tool for tumor diagnosis as well as for targeting tumors.

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Dependence of Energy Metabolism on the Density of Cells in Culture.

Cited by 45 publications

References 23 publications

Influence of HEK293 metabolism on the production of viral vectors and vaccine

Influence of HEK293 metabolism on the production of viral vectors and vaccine

Temporal changes of multiple redox couples from proliferation to growth arrest in IEC-6 intestinal epithelial cells

Heterogeneity in tumor cell energetic metabolome at different cell cycle phases of human colon cancer cell lines

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