Dependence of operational parameters of laccase-based biosensors on structure of photocross-linked polymers as holding matrixes

Kavetskyy, Taras; Smutok, Oleh; Demkiv, Olha; Kašėtaitė, Sigita; Ostrauskaitė, Jolita; Švajdlenková, Helena; Šauša, O.; Zubrytska, K.; Hoivanovych, Nataliia; Gonchar, Mykhailo

doi:10.1016/j.eurpolymj.2019.03.056

Cited by 17 publications

(23 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The photocross-linked polymers based on the epoxidized linseed oil (ELO), bisphenol A diglycidyl ether (RD), and triarylsulfonium hexafluorophosphates used as photoinitiator (PI) were synthesized by the reaction of ELO and 10 mol.% or 30 mol.% of RD, using 3 mol.% of PI (ELO/10RD and ELO/30RD) as reported in [5].…”

Section: Methodsmentioning

confidence: 99%

“…The swelling tests were performed during 8 days for the ureasil-based polymers and 2 days for the photocrosslinked polymers at room temperature. Details of swelling experiment were reported in [2,4,5].…”

Section: Methodsmentioning

confidence: 99%

“…In case of the ureasil-based polymers, planar gold electrodes DRP-C220AT from "DropSens" (Llanera, Asturias, Spain) were used as working electrodes [1,3]. In case of the photocross-linked polymers, graphite rods (type RW001, 3.05 mm diameter, area 7.3 mm 2 , Ringsdorff Werke, Bonn, Germany) were used as working electrodes [5]. Immobilization of laccase was described for the ureasil-based polymers in [1,3] and for the photocross-linked polymers in [5].…”

Section: Methodsmentioning

confidence: 99%

“…In case of the photocross-linked polymers, graphite rods (type RW001, 3.05 mm diameter, area 7.3 mm 2 , Ringsdorff Werke, Bonn, Germany) were used as working electrodes [5]. Immobilization of laccase was described for the ureasil-based polymers in [1,3] and for the photocross-linked polymers in [5].…”

Section: Methodsmentioning

confidence: 99%

“…At the same time, positron annihilation lifetime spectroscopy (PALS) is known as a progressive method for microstructural analysis of macromolecular structures. Combination of both methods allowed to get information about network properties of the polymer matrices and the results obtained will be further compared with sensitivity of bioelectrodes constructed using the polymer matrices [1][2][3][4][5]. A role of free-volume and crosslink density in the host polymer matrices used for improvement of operational parameters of laccase-based amperometric biosensors would be established.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Controlling the Network Properties of Polymer Matrices for Improvement of Amperometric Enzyme Biosensors: Contribution of Positron Annihilation

et al. 2020

View full text Add to dashboard Cite

Recent results obtained by studies on the network properties using positron annihilation lifetime spectroscopy and swelling measurements in the ureasil-based and photocross-linked polymer matrices are compared. Common features observed are the larger difference (αF2 − αF1) of the coefficients for the thermal expansion of free-volume holes αF1, αF2 below and above Tg, respectively, in the polymer matrices, the highest sensitivity of the amperometric enzyme biosensors constructed based on the polymer matrices. It is suggested that the correlation found may be more general. It seems, therefore, that controlling the network properties of polymer matrices suitable for construction of amperometric enzyme biosensors by examining (αF2 − αF1) magnitude in temperature dependent positron annihilation lifetime spectroscopy experiment may be used for improvement of operational parameters of such sensors.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Controlling the Network Properties of Polymer Matrices for Improvement of Amperometric Enzyme Biosensors: Contribution of Positron Annihilation

et al. 2020

View full text Add to dashboard Cite

show abstract

Extracellular laccase from Monilinia fructicola: isolation, primary characterization and application

Demkiv

Gayda

Broda

et al. 2020

Cell Biology International

View full text Add to dashboard Cite

Laccases are enzymes belonging to the family of blue copper oxidases. Due to their broad substrate specificity, they are widely used in many industrial processes and environmental bioremediations for removal of a large number of pollutants. During last decades, laccases attracted scientific interest also as highly promising enzymes to be used in bioanalytics. The aim of this study is to obtain a highly purified laccase from an efficient fungal producer and to demonstrate the applicability of this enzyme for analytics and bioremediation. To select the best microbial source of laccase, a screening of fungal strains was carried out and the fungus Monilinia fructicola was chosen as a producer of an extracellular enzyme. Optimal cultivation conditions for the highest yield of laccase were established; the enzyme was purified by a column chromatography and partially characterized. Molecular mass of the laccase subunit was determined to be near 35 kDa; the optimal pH ranges for the highest activity and stability are 4.5–5.0 and 3.0–5.0, respectively; the optimal temperature for laccase activity is 30°C. Laccase preparation was successfully used as a biocatalyst in the amperometric biosensor for bisphenol A assay and in the bioreactor for bioremediation of some xenobiotics.

show abstract

Novel Photocross-Linked Polymers for Construction of Laccase-Based Amperometric Biosensors

Kavetskyy

Zubrytska

Smutok

et al. 2020

NATO Science for Peace and Security Series B: Physics and Biophysics

View full text Add to dashboard Cite

Dependence of operational parameters of laccase-based biosensors on structure of photocross-linked polymers as holding matrixes

Cited by 17 publications

References 32 publications

Controlling the Network Properties of Polymer Matrices for Improvement of Amperometric Enzyme Biosensors: Contribution of Positron Annihilation

Controlling the Network Properties of Polymer Matrices for Improvement of Amperometric Enzyme Biosensors: Contribution of Positron Annihilation

Extracellular laccase from Monilinia fructicola: isolation, primary characterization and application

Novel Photocross-Linked Polymers for Construction of Laccase-Based Amperometric Biosensors

Contact Info

Product

Resources

About