Dependence of Platelet Thrombus Stability on Sustained Glycoprotein IIb/IIIa Activation Through Adenosine 5′-Diphosphate Receptor Stimulation and Cyclic Calcium Signaling

Abstract

Continuous P2Y1 and P2Y12 stimulation resulting in cyclic [Ca2+]i oscillations is required for maintaining the activation of GP IIb/IIIa needed for thrombus stability in flowing blood.

Rapid resensitization of purinergic receptor function in human platelets

“…As a consequence of this rapid resensitization, platelets are able to retain their reactivity at sites of vessel injury even if they have already been exposed previously to ADP, thereby sustaining hemostatic function. Recent studies have suggested that continuous ADP signaling is required for thrombus stability [37,38]. It is unclear at present how the processes and timing of P2Y desensitization and subsequent resensitization link with those of thrombus formation and subsequent stability.…”

Imaging of Structural Changes in Endothelial Cells and Thrombus Formation at the Site of FeCl₃-Induced Injuries in Mice Cremasteric Arteries

“…As previously demonstrated, platelet ADP receptor P2Y12 plays a crucial role in maintaining the stability of platelet thrombi under blood flow conditions 21,23,33,34) , while the cyclo-oxygenase pathway, which was blocked by aspirin, does not play a major Experiments were conducted similar to as described in the legend for Fig. 2.…”

“…The 3D images of thrombi developed at the site of FeCl3-induced endothelial damage were detected in real time with our newly-developed real-time 3D imaging system using ultra-fast confocal microscopy equipped with a piezo-motor control unit 21) . 3D growth of thrombi was continuously recorded with a personal computer (PowerMac, Apple, Co., USA).…”

Important Regulatory Role of Activated Platelet-Derived Procoagulant Activity in the Propagation of Thrombi Formed Under Arterial Blood Flow Conditions

“…[25][26][27] Clinically relevant anticoagulants, including unfractionated heparin and low-molecular-weight heparin, were also tested, but the results could not be included because the blood coagulated during experiments.…”