Dependence of reactivity and cooperativity in normal human erythrocyte glucose-6-phosphate dehydrogenase on ionic strength, pH, and temperature

Adediran, S. A.

doi:10.1007/bf01025235

Cited by 4 publications

(1 citation statement)

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“…However, it must be established whether the relative inability of trehalose to partition into the membrane/permeate the RBC limited its abilities to protect against the injury at −4°C, or whether its resulting functionality as an impermeant osmotic agent (relative to PEG400) impeded any protection it could impart. With respect to the latter, an increase in RBC shrinkage could promote activation of non-selective, volume-dependent cation channels, leading to high intracellular Cl-concentrations, which would cause intracellular acidification and a further decrease in the activation energy required for the reduction of major RBC antioxidant systems ( Adediran, 1983 ; Huber et al, 2001 ). On the other hand, partitioning of the Lo phase could minimize low temperature-induced aggregation of Lo domains (and the damage that would result from this), while permeation of PEG400 could limit irreversible denaturation of proteins/macromolecules known to be prominent during prolonged supercooled storage through the formation of hydration shells ( Privalov, 1990 ).…”

Section: Discussionmentioning

confidence: 99%

Comparing two extracellular additives to facilitate extended storage of red blood cells in a supercooled state

et al. 2023

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Background: Adenosine triphosphate (ATP) levels guide many aspects of the red blood cell (RBC) hypothermic storage lesions. As a result, efforts to improve the quality of hypothermic-stored red cell concentrates (RCCs) have largely centered around designing storage solutions to promote ATP retention. Considering reduced temperatures alone would diminish metabolism, and thereby enhance ATP retention, we evaluated: (a) whether the quality of stored blood is improved at −4°C relative to conventional 4°C storage, and (b) whether the addition of trehalose and PEG400 can enhance these improvements.Study Design and Methods: Ten CPD/SAGM leukoreduced RCCs were pooled, split, and resuspended in a next-generation storage solution (i.e., PAG3M) supplemented with 0–165 mM of trehalose or 0–165 mM of PEG400. In a separate subset of samples, mannitol was removed at equimolar concentrations to achieve a fixed osmolarity between the additive and non-additive groups. All samples were stored at both 4°C and −4°C under a layer of paraffin oil to prevent ice formation.Results: PEG400 reduced hemolysis and increased deformability in −4°C-stored samples when used at a concentration of 110 mM. Reduced temperatures did indeed enhance ATP retention; however, in the absence of an additive, the characteristic storage-dependent decline in deformability and increase in hemolysis was exacerbated. The addition of trehalose enhanced this decline in deformability and hemolysis at −4°C; although, this was marginally alleviated by the osmolarity-adjustments. In contrast, outcomes with PEG400 were worsened by these osmolarity adjustments, but at no concentration, in the absence of these adjustments, was damage greater than the control.Discussion: Supercooled temperatures can allow for improved ATP retention; however, this does not translate into improved storage success. Additional work is necessary to further elucidate the mechanism of injury that progresses at these temperatures such that storage solutions can be designed which allow RBCs to benefit from this diminished rate of metabolic deterioration. The present study suggests that PEG400 could be an ideal component in these solutions.

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Section: Discussionmentioning

confidence: 99%