To inform room-temperature spin functionality of D03-ordered Fe3Si, we explore tunneling magnetoresistance (TMR) effects in magnetic tunnel junctions (MTJs) consisting of Co60Fe40/AlO
x
/Fe3Si on Si. We clearly observe reproducible TMR ratios of ∼20% at room temperature for all MTJs fabricated. By using Julliere's formula, the room-temperature spin polarization (P) value for D03-ordered Fe3Si can be roughly estimated to be ranging from 0.18 to 0.45 when we assume the P values from 0.5 to 0.2 for Co60Fe40. This study reveals that the room temperature P value for D03-ordered Fe3Si is relatively small compared with that for Co-based Heusler alloys reported previously.