Dependence of spontaneous reversion frequencies in haploid yeasts of different genotypes on the concentration of adenine in the medium and on the age of the culture

Ilyina, V.L.; Korogodin, V.I.; Fajszi, Cs.

doi:10.1016/0165-7992(86)90149-1

Cited by 7 publications

(9 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…When we discovered that the rate of occurrence of adenine revertants increases with decreasing adenine content in the medium (Ilyina et al, 1985(Ilyina et al, , 1986, we supposed that this phenomenon might be interpreted in terms of the hypothesis of nucleotide pool imbalance in its simplest form (Bresler et al, 1973;Haynes, 1985). This hypothesis also agrees with the fact that mutability of genes is determined by the conditions during DNA replication alone (cf.…”

Section: Spontaneous Mutability and The Hypothesis Of Nuckotide Pool mentioning

confidence: 54%

“…Some years ago we showed (Ilyina et a/., 1985(Ilyina et a/., , 1986) that the reversion rate in adenine auxotrophic yeasts increases with decreasing adenine content of the medium. In one strain it was shown (Ilyina et a/., 1987) that the reversion rates reflecting mutations in different genes (in gene ade2 or in the suppressor genes) are differently influenced by the concentration of adenine in the medium.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

On the dependence of spontaneous mutation rates on the functional state of genes

Korogodin

Korogodina

Fajszi

et al. 1991

Yeast

Self Cite

View full text Add to dashboard Cite

Spontaneous mutation of some genes was studied in haploid adenine and leucine auxotrophic yeast Saccharomyces. It was shown that a decrease in the amount of adenine (from 500 to 0 mg l-1) or leucine (from 300 to 0.3 mg l-1) in the medium, simultaneously with the transition from repression to derepression of the biosynthesis of these metabolites, resulted in a 15- to 150-fold increase in the reversion rate of genes ade 2 and leu2, respectively, for different strains. At the same time the mutation rate of suppressor genes varied relatively little (up to five-fold), and that of gene lys did not change at all. It was also demonstrated (on gene leu2) that the mutation rate is determined by the composition of the nutrient medium at the time of the S-phase of the cell cycle and it does not depend on the cultivation conditions during the presynthetic period. We discuss the hypothesis that derepressed genes mutate with a significantly higher rate than genes in the repressed state.

show abstract

Section: Spontaneous Mutability and The Hypothesis Of Nuckotide Pool mentioning

confidence: 54%

Section: Introductionmentioning

confidence: 99%

On the dependence of spontaneous mutation rates on the functional state of genes

Korogodin

Korogodina

Fajszi

et al. 1991

Yeast

Self Cite

View full text Add to dashboard Cite

show abstract

“…In the special case of starvation for the nucleotide precursor adenine, it was reasoned that an imbalance of the nucleotide pool might develop, and consequently enhance misincorporation of nucleotides during repair DNA synthesis (Ilyina et al, 1986). However, no definite proof of this assumption has been determined.…”

Section: The Poorly Defined State Of Starving Cellsmentioning

confidence: 98%

Adaptive Mutation inSaccharomyces cerevisiae

Heidenreich

2007

Critical Reviews in Biochemistry and Molecular Biology

View full text Add to dashboard Cite

Adaptive mutation is a generic term for processes that allow individual cells of nonproliferating cell populations to acquire advantageous mutations and thereby to overcome the strong selective pressure of proliferation-limiting environmental conditions. Prerequisites for an occurrence of adaptive mutation are that the selective conditions are nonlethal and that a restart of proliferation may be accomplished by some genetic change in principle. The importance of adaptive mutation is derived from the assumption that it may, on the one hand, result in an accelerated evolution of microorganisms and, on the other, in multicellular organisms may contribute to a breakout of somatic cells from negative growth regulation, i.e., to cancerogenesis. Most information on adaptive mutation in eukaryotes has been gained with the budding yeast Saccharomyces cerevisiae. This review focuses comprehensively on adaptive mutation in this organism and summarizes our current understanding of this issue.

show abstract

“…Seeds were collected in a 30-km zone around the NPP in years with normal high (1998) and extreme high summertime temperatures (1999) [14,39]. The statistical modeling was performed on the appearance of CAs in seedling meristem cells and of cells with 1 The cells stimulated to proliferate are involved in the distributions.…”

Section: Adaptation and The Risk Of Genome Instability In Ecologymentioning

confidence: 99%

“…At present, there are numerous radiation sources around the world and low irradiation doses and dose rates are common environmental factors. In the 1990s it was found that such stresses are accompanied by time-dependent genetic instabilities, which include spatial intracellular [1][2][3][4] and intercellular [5][6][7][8][9] spreading of DNA damage. It is important that mutations or chromosomal aberrations can accumulate in cells that experience stress conditions [10] due to the stimulation of mismatch repair [11].…”

Section: Introductionmentioning

confidence: 99%

Adaptation and Radiation-Induced Chromosomal Instability Studied by Statistical Modeling~!2009-12-10~!2010-02-20~!2010-03-30~!

Korogodina¹,

Florko²,

Osipova³

2010

TOEVOLJ

View full text Add to dashboard Cite

It is known that irradiation stress induces late intracellular processes and adaptation. In this paper, we formulate the adaptation hypothesis. On its basis, we present statistical modeling of cellular distributions on the number of chromosomal abnormalities in root meristems of pea seeds that experienced low-dose-rate irradiation, high temperatures, and aging. The modeling shows that multiple appearances of chromosomal abnormalities can be explained as a result of primary or late processes caused by DNA damage, with or without cell selection, which can be described with geometrical or Poisson distributions, respectively. The connections between the late intercellular processes (the "bystander effects") and late intracellular regulatory processes are considered. It is shown that combinations of low-dose-rate irradiation, high temperatures, and aging lead to synergistically increasing instabilities and decreasing seed survivals. Approaches to risk estimation of chromosomal instability are investigated in plant cells and in human blood lymphocytes. Risks of instability were estimated as values of distribution parameters dependent on radiation intensity in ecology and in generations of persons who experienced radioactive fallouts from nuclear tests. We conclude that statistical model of adaptation describes intraand intercellular processes of genetic instability coupled with selection, and that the risks of these processes can be calculated as model parameters.

show abstract

Dependence of spontaneous reversion frequencies in haploid yeasts of different genotypes on the concentration of adenine in the medium and on the age of the culture

Cited by 7 publications

References 6 publications

On the dependence of spontaneous mutation rates on the functional state of genes

On the dependence of spontaneous mutation rates on the functional state of genes

Adaptive Mutation inSaccharomyces cerevisiae

Adaptation and Radiation-Induced Chromosomal Instability Studied by Statistical Modeling~!2009-12-10~!2010-02-20~!2010-03-30~!

Contact Info

Product

Resources

About