Dependence of water uptake and morphology of hyaluronan- and alginate-based hydrogels on pH and degree of crosslinking

Barbucci, Rolando; Consumi, Marco; Magnani, Agnese

doi:10.1002/1521-3935(200207)203:10/11<1292::aid-macp1292>3.0.co;2-i

Cited by 20 publications

(24 citation statements)

References 17 publications

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“…The procedure to crosslink the amidic derivative was identical to that used for the amidation, with the only exception that the crosslinking agent (1.3 diaminopropane) instead of the amidating agent (methylamine) was used. The hydrogel obtained was purified by following washings with bidistilled water and ethanol 18, 19…”

Section: Methodsmentioning

confidence: 99%

“…The hydrogel obtained was purified by following washings with bidistilled water and ethanol. 18,19 Physical-chemical characterization…”

Section: Crosslinking Of the Polysaccharidementioning

confidence: 99%

“…ATR spectra of the polymer and the hydrogel in dry form were recorded on a Bio-rad FTS6000 apparatus between 4000 and 750 cm À1 , following the classic procedure. 19…”

Section: Ftir-atr Analysismentioning

confidence: 99%

“…Amidic alginate was crosslinked using a previously reported procedure. 26,19 A hydrogel with a theoretic crosslinking degree of 100% (the remaining carboxylate groups after the amidation were 63%) was realized (AAA_cr). However, the infrared analysis showed a very small peak corresponding to free carboxylic groups, demonstrating that a complete crosslinking of AAA was not achieved.…”

Section: Amidic Alginate Crosslinking (Aaa_cr)mentioning

confidence: 99%

“…However, the infrared analysis showed a very small peak corresponding to free carboxylic groups, demonstrating that a complete crosslinking of AAA was not achieved. 19…”

Section: Amidic Alginate Crosslinking (Aaa_cr)mentioning

confidence: 99%

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Amidic alginate hydrogel for nucleus pulposus replacement

Leone

Torricelli

Chiumiento

et al. 2007

J Biomedical Materials Res

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Degeneration of intervertebral discs is the most common cause of back pain. The first phase of this degenerative process involves the nucleus pulposus (NP). A rapid recovery of this structure can prevent further degradation of the annulus fibrosus. A new amidic derivative of alginate (AAA) was developed to obtain a polysaccharide possessing some of the physical-chemical properties of Hyal (i.e. viscosity) without losing the rigidity of the native alginate structure. The modified polysaccharide was crosslinked using 1.3 diaminopropane as crosslinking agent. The hydrogel obtained was characterized in terms of water uptake and rheological behavior. In particular, the viscoelastic behavior of the hydrogel was determined in shear stress under dynamic conditions and compared with the behavior of nondegenerated human lumbar NP. We then assessed the effect of the AAA hydrogel on NHC (Normal Human Chondrocyte) cell viability and on the production of important extracellular matrix factors, such as glycosaminoglycans and Type II collagen. In conclusion, the results achieved in this study demonstrated that the amidic alginate-based scaffold is a promising material to be utilized in the replacement of NP.

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Section: Methodsmentioning

confidence: 99%

“…The hydrogel obtained was purified by following washings with bidistilled water and ethanol. 18,19 Physical-chemical characterization…”

Section: Crosslinking Of the Polysaccharidementioning

confidence: 99%

“…ATR spectra of the polymer and the hydrogel in dry form were recorded on a Bio-rad FTS6000 apparatus between 4000 and 750 cm À1 , following the classic procedure. 19…”

Section: Ftir-atr Analysismentioning

confidence: 99%

Section: Amidic Alginate Crosslinking (Aaa_cr)mentioning

confidence: 99%

“…However, the infrared analysis showed a very small peak corresponding to free carboxylic groups, demonstrating that a complete crosslinking of AAA was not achieved. 19…”

Section: Amidic Alginate Crosslinking (Aaa_cr)mentioning

confidence: 99%

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Amidic alginate hydrogel for nucleus pulposus replacement

Leone

Torricelli

Chiumiento

et al. 2007

J Biomedical Materials Res

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Hydrogel membranes based on gum tragacanth with tunable structures and properties. II. Comprehensive characterization of the swelling behavior

Kiani

Asempour

2012

J of Applied Polymer Sci

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Hydrogel membranes based on gum tragacanth (GT), with a wide range of crosslinking densities (0.83-23.87 Â 10 À6 mol/cm 3 ) and swelling capacities (120-1580 g/g), were prepared with the aid of heat alone and four crosslinking reagents according to a procedure reported in our previous article. In this article, we report on the comprehensive evaluation of the swelling behavior of the prepared hydrogel membranes. These include the swelling kinetics, swelling behavior in terms of the pH and ionic strength of the swelling media, water absorbency under load (AUL), swelling as a function of sample size, deswelling behavior, and successive swelling and deswelling cycles. The swelling kinetics of the hydrogel membranes with higher crosslinking densities and lower swelling equilibria were closer to those of the case II transport mechanism; this is desirable in the field of drug-delivery systems (DDSs). Furthermore, higher crosslinking densities, and hence stronger structures, of the membranes led to higher AUL values and more stable performances in the successive swelling and deswelling cycles. Moreover, because of the anionic nature of the gum, the prepared GT hydrogels showed pH-and ionic-strengthdependent swelling behavior. All of these results reveal that GT could be a promising material for the preparation of hydrogel membranes for applications in the fields of DDS, tissue engineering, and the separation of small molecules.

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Formation of defined microporous 3D structures starting from cross‐linked hydrogels

Barbucci

Leone

2003

J Biomed Mater Res

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A new and simple technique was developed to obtain polysaccharide (hyaluronane, alginate and carboxymethylcellulose) -based hydrogels with a defined porous morphology. The technique consists of stratifying a cross-linked hydrogel on a filter with known pore diameter. CO(2) bubbles, produced by the addition of HCl to a porogen salt NaHCO(3), are forced to pass through the filter, and they induce the hydrogel to assume a porous morphology. The presence and distribution of pores was confirmed by scanning-electron microscopy (SEM). A strict correspondence was found between the porosity of the filter and the pore diameter in the hydrogels. Water uptake measurements showed a decreased amount of water taken up by the porous hydrogels compared with the native hydrogels, due to a compacting of the material. An explanation of the porous material properties of Hyal hydrogel was given on the basis of FTIR spectra.

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Dependence of water uptake and morphology of hyaluronan- and alginate-based hydrogels on pH and degree of crosslinking

Cited by 20 publications

References 17 publications

Amidic alginate hydrogel for nucleus pulposus replacement

Amidic alginate hydrogel for nucleus pulposus replacement

Hydrogel membranes based on gum tragacanth with tunable structures and properties. II. Comprehensive characterization of the swelling behavior

Formation of defined microporous 3D structures starting from cross‐linked hydrogels

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