Dependending of biochemical activities Mycobacterium bovis on passages and temperature of cultivation

Abstract

The tuberculosis of cattle remains a serious problem both in Ukraine and for the whole world for many years. In spite of the fact that in science now much known about the agent of tuberculosis, some of the mechanisms through which mycobacteria manage to survive in the conditions of macroorganism and the environment have not been studied. Difficulties in overcoming of tuberculosis and mycobacteriosis can be connected with the incredible plasticity of life processes within the bacterial cells of mycobacteria. Understanding the enzymatic processes in the bacterial cell and their changes in response to the influence of external factors can lead to an understanding of the complex mechanism of adaptation and survival of mycobacteria, both in macroorganism and in the environment. The aim of our research was to determine the influence of the number passage of high-resistance strain Mycobacterium bovis in the organism of guinea pigs (Cavia porcellus), the temperature of cultivation (3°C; 37°C) on changes in biochemical activity. The ability of M. bovis and dissociative forms to grow on a nutrient medium with addition of sodium salicylate and on usual nutrient media (meat-peptone extract and meat-peptone agar) was investigated. The research was carried out using the high-virulence strain M. bovis, which was stored in the museum of the Laboratory of the Department of epizootology and infectious diseases of Dnipro State Agrarian and Economic University during 10-13 years, as well as dissociative forms 117-a, b, v, 118. The results of the research indicate the ability of mycobacteria to change biochemical activity under the influence of external factors. It has been established that the number of passages through a dense egg nutrient medium does not have a direct effect on the enzymatic activity of M. bovis. In dissociative forms with increasing number of inoculation of subcultures, the activity of nitrate reductase increases and peroxidase activity, dehydrogenase activity and catalase activity decrease. It has been found that low positive culturing temperature (3 ºС) contributes to increase of catalase activity and hydrolysis ability of Tween-80. It has been determined that after passage through the organism of guinea pigs, M. bovis increase the activity of dehydrogenase and catalase and reduce nitrate reductase activity and hydrolysis ability of TWIN-80. So, the results presented in the article shows that the existence of mycobacteria is able to change the function of their own systems according to the changes of environment conditions..

The ability of Sitophilus oryzae (Coleoptera, Curculionidae) to transmit Mycobacterium bovis: Morphology, cultural biochemical properties of the bacteria

“…During the study of biochemical activity, we identified the catalase, peroxidase, dehydrogenase activities, presence of reduction of nitrates, and ability to hydrolize Tween 80. Catalase and peroxidase activities were identified using the modified Bogen's technique (Tkachenko et al, 2019). Into test tubes with the culture, we added 2% solution of hydrogen peroxide and 0.5% solution of pyrogallol A.…”

“…The studies of dehydrogenase activity were carried out using the Bloch technique (1950), modified by Tkachenko et al (2019). We gathered 4.0 cm 3 of weighed amount of the culture of microbial cells with the concentration of 10.0 mg/cm 3 in phosphate buffer (рН 7.4-7.6), mixed with 1.0 cm 3 of 1.0% of glucose solution and 0.1 cm 3 of 0.02% solution of methylene blue.…”

“…At the same time, we made impression smears from the internal organs, fixated them and stained according to the Ziehl-Neelsen method. Also, we prepared a suspension from damaged organs according to the method of Alikaieva (1950): the suspension was inoculated onto the Stonebrink medium (Tkachenko et al, 2019). The inoculations were incubated at +37.0 ± 0.5 °С.…”