1992
DOI: 10.1139/o92-186
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Dephosphorylation of the retinoblastoma protein during differentiation of HL60 cells

Abstract: Immunoprecipitated retinoblastoma protein from HL60 cells migrated as a series of bands during electrophoresis. The heterogeneity appeared to be generated by phosphorylation of the retinoblastoma protein. Treatment of the cells with the phorbol ester, tetradecanoyl phorbol acetate (TPA), resulted in both a loss of the heterogeneity of the pRB species and a significant decrease in the level of pRB phosphorylation. These changes accompanied differentiation of the HL60 cells into macrophages. Treatment of the cel… Show more

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Cited by 12 publications
(6 citation statements)
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“…In contrast, activation of PKC in late G 1 maintained pRb in a hypophosphorylated state and inhibited G 1 →S phase progression. The inhibitory effect of PKC activation on growth factor-induced pRb phosphorylation was also seen in vascular smooth muscle cells (52), intestinal epithelial cells (45), HL60 myelocytic leukemia cells (82), and LNCaP prostate epithelial cells (83), as well as in NIH3T3 cells stably overexpressing PKC eta (50) and vascular smooth muscle cells overexpressing PKC delta (84).…”
Section: Pkc and Regulation Of The G 1 →S Transitionmentioning
confidence: 94%
“…In contrast, activation of PKC in late G 1 maintained pRb in a hypophosphorylated state and inhibited G 1 →S phase progression. The inhibitory effect of PKC activation on growth factor-induced pRb phosphorylation was also seen in vascular smooth muscle cells (52), intestinal epithelial cells (45), HL60 myelocytic leukemia cells (82), and LNCaP prostate epithelial cells (83), as well as in NIH3T3 cells stably overexpressing PKC eta (50) and vascular smooth muscle cells overexpressing PKC delta (84).…”
Section: Pkc and Regulation Of The G 1 →S Transitionmentioning
confidence: 94%
“…[4][5][6][7] The increased expression of p21 was suggested to contribute to the arrest cell cycle progression during the differentiation through changing the state of phosphorylation levels of cell cycle-related gene products, such as RB protein. 8) Induction of the expression of p21 gene is classified into two types: One is p53 dependent induction observed with DNA damage, such as exposure to radiation and the other is p53-independent induction. Since HL-60 cells lack the p53 gene, expression of p21 was suggested to be induced via a p53-independent mechanism.…”
mentioning
confidence: 99%
“…The increased expression of p21 was suggested to contribute to the arrest of cell cycle progression during the differentiation through changing the state of phosphorylation levels of cell cycle-related gene products, such as RB protein [17] . p27 and p21 have the high homology, they suppress the process of cyclin-dependent kinase activation and the cyclin-CDK compound activity [18][19][20] .…”
Section: Discussionmentioning
confidence: 99%
“…HL-60 cells lack the normal functional p53 gene. The expression of p21 is suggested to be induced via a p53-independent mechanism [21] . P21 and p27 are important negative regulatory molecules.…”
Section: Discussionmentioning
confidence: 99%