Dephosphorylation of αS- and κ-Caseins and Its Effect on Micelle Stability in the κ-αS-Casein System

Modifications of structures and functions of caseins: a scientific and technological challenge

“…As other consequences of this dephosphorylation, the charge distribution along the protein chain was also altered affecting the auto-association of caseins. Besides, the calcium binding and precipitability in the presence of calcium were reduced (Pepper and Thompson 1963;Yamauchi et al 1967;Bingham 1976;Molina et al 2007;McCarthy et al 2013). The loss of phosphate groups reduced the number of calcium bridges (Aoki et al 1985).…”

“…The loss of phosphate groups reduced the number of calcium bridges (Aoki et al 1985). Formation of artificial micelles with dephosphorylated caseins was difficult because of the removal of the majority of calcium-binding sites (Pepper and Thompson 1963;Yamauchi et al 1967;Bingham et al 1972;Pearse et al 1986;McCarthy et al 2013) and resulted in the formation of abnormal micelles less stable in presence of calcium and smaller in size (Pepper and Thompson 1963;Knoop and Peters 1975;Pearse et al 1986;Hiller and Lorenzen 2009).…”

Purification and Characterization of UDP-N-Acetylgalactosamine: κ-Casein PolypeptideN-Acetylgalactosaminyltransferase from Mammary Gland of Lactating Cow

“…We have reported that phosphorylation of K-casein terminates before glycosylation, using enzymes in the Goigi apparatus of lactating mammary glands from rat and cow. 6 ) Pepper et al 20 ) and we 2 l) indicated that micelles are formed after phosphorylation. Then, which one occurs first, micelle formation or glycosylation ?…”

Post-translational Modification of κ-Casein in the Golgi Apparatus of Mid-lactating Mammary Glands from Rat and Cow

“…Pepper et at. 35) and we 36 ) have shown that phosphorylation of a sl -and f3-casein is an indispensable step for micelle assembly. These results suggested that phosphorylation occurs before the micelle assembly.…”