Depigmenting effect of Sterculia lynchnophera on B16F10 melanoma and C57BL/6 melan-a cells

Abstract

To develop a novel skin depigmenting agent from natural sources, the inhibition of melanogenesis by the Chinese herb, Sterculia lynchnophera (SL), was evaluated. Treatment of B16F10 melanoma cells and melan-a cells with SL exhibited a 32.9% and 68.2% inhibition of melanin synthesis without cytotoxicity at a concentration of 200 µg/ml, respectively. This herb possessed a high free radical scavenging activity with IC 50 =11.02 µM. The methanol extract of SL slightly inhibited in vitro mushroom tyrosinase activity (23.4% at a concentration of 200 µg/ml) and had a significant inhibitory effect on cellular tyrosinase activivity (48.65% and 88.56% inhibition at the concentration 200 µg/ml in B16F10 cells and C57BL/6 melan-a cells, respectively). From the western blotting results, SL inhibited the expression of tyrosinase and tyrosinase related protein 1 (TRP-1). Taken together, we suggest that SL may be a safe and effective depigmentation agent.

Depigmenting Effect of Kojic Acid Esters in Hyperpigmented B16F1 Melanoma Cells

“…Melanin synthesis can also be induced by the presence of free radicals and reactive species. Excessive explore of ultraviolet radiation, metal ions, free radicals, and reactive species have significantly stimulate transcription of tyrosinase gene and contribute to hyperpigmentation [ 4 , 39 ]. The presence of metal ions, free radical, and reactive species caused oxidation in melanogenesis pathway that will result in high melanin synthesis [ 8 ].…”

Sterculia and Brachychiton: a comprehensive overview on their ethnopharmacology, biological activities, phytochemistry and the role of their gummy exudates in drug delivery

“…The extract also showed a high free radical scavenging activity (IC 50 = 11.02 lM), and thus, it showed a great potential to be applied as a skin depigmenting agent. [85] Sterculia villosa which was used traditionally as diuretic showed potent diuretic effect in a dose-dependent manner when examined using healthy Wistar rats and rabbits at doses of 100, 200 and 400 mg/kg of the ethanolic bark extract, and the results were comparable to the reference drug furosemide (20 mg/kg). [77] The diuretic effect occurred by increasing urine output with increased excretion of sodium and potassium salts thus can be useful for decreasing blood pressure, cardiac overload and pulmonary congestion.…”

“…[119] Moreover, Sterculia gum was evaluated as a hydrogel matrix in dressings containing tetracycline antibiotic used for wound healing. Dressings showed an effective antimicrobial effect via two mechanisms, first due to the controlled release of the antimicrobial drug from the gum matrix and the second was attributed to the antimicrobial (85) S. foetida [96] action of Sterculia gum. [120] Also, skin patches for the treatment of warts or papilloma used S. urens gum for transdermal drug delivery and salicylic acid for its keratolytic activity.…”