Depletion of Alpha-Melanocyte-Stimulating Hormone Induces Insatiable Appetite and Gains in Energy Reserves and Body Weight in Zebrafish

Hsieh, Yang Wen; Tsai, Yun-An; Lai, Hsin Hung; Lai, Chien-Yu; Lin, Chiu Ya; Her, Guor Mour

doi:10.3390/biomedicines9080941

Cited by 10 publications

(5 citation statements)

References 66 publications

(81 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“… Note: it is to be noted that in studies involving drug administration or genetic modifications where the larval feed uptake may vary as a response, control feeding assays should be performed in the animals for rational validation. Various researches have utilized fluorescently labeled viable paramecia such as 4-10-Di-ASP-labeled paramecia, fish food coated fluorescent microspheres or fluorescent Tetrahymena to evaluate the feed intake, especially in larvae, in view of their transparent body ( Shimada et al., 2012 ) ( Boyer et al., 2013 ) ( Hsieh et al., 2021 ) ( Hsieh et al., 2021 ). For appropriate HFD controls in hyperlipidemic studies, high fat feed serves are fluorescently (NBD- and BODIPY-Cholesterol) or radioactively labeled (14C-labeled lipids) to monitor lipid trafficking in live larvae, which can be used to indirectly measure lipid (feed) uptake ( Sæle et al., 2018 ) ( Anderson et al., 2016 ).…”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

Protocol to evaluate hyperlipidemia in zebrafish larvae

et al. 2022

View full text Add to dashboard Cite

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

Protocol to evaluate hyperlipidemia in zebrafish larvae

et al. 2022

View full text Add to dashboard Cite

“…The expression of all selected genes was performed using CFX Connect System (Bio-Rad, Hercules, CA, USA) with SYBR Premix EX Taq TM II (TaKaRa). The GenBank accession numbers for the selected genes and primer sequences were previously reported [ 16 , 27 , 28 , 29 , 30 , 33 , 34 , 35 ].…”

Section: Methodsmentioning

confidence: 99%

“…Growth rate studies were performed,, as described previously [ 16 , 28 ]. The growth curve was measured monthly from 2 months post fertilization (mpf) to 12 mpf.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

MXD3 Promotes Obesity and the Androgen Receptor Signaling Pathway in Gender-Disparity Hepatocarcinogenesis

Tsai

Jeng

He³

et al. 2021

Cells

Self Cite

View full text Add to dashboard Cite

Obesity is closely linked to metabolic diseases, particularly non-alcoholic steatohepatitis (NASH) or non-alcoholic fatty liver disease (NAFLD), ultimately leading to hepatocellular carcinoma (HCC). However, the molecular mechanisms of NASH-associated HCC (NAHCC) remain elusive. To explore the impact of Max dimerization protein 3 (MXD3), a transcription factor that regulates several cellular functions in disorders associated with metabolic diseases, we conditionally expressed Mxd3 proteins using Tet-on mxd3 transgenic zebrafish (MXs) with doxycycline (MXs + Dox) or without doxycycline (MXs − Dox) treatment. Overexpression of global MXD3 (gMX) or hepatic Mxd3 (hMX) was associated with obesity-related NAFLD pathophysiology in gMX + Dox, and liver fibrosis and HCC in hMX + Dox. Oil Red O (ORO)-stained signals were seen in intravascular blood vessels and liver buds of larval gMX + Dox, indicating that Mxd3 functionally promotes lipogenesis. The gMX + Dox-treated young adults exhibited an increase in body weight and visceral fat accumulation. The hMX + Dox-treated young adults showed normal body characteristics but exhibited liver steatosis and NASH-like phenotypes. Subsequently, steatohepatitis, liver fibrosis, and NAHCC were found in 6-month-old gMX + Dox adults compared with gMX − Dox adults at the same stage. Overexpression of Mxd3 also enhanced AR expression accompanied by the increase of AR-signaling pathways resulting in hepatocarcinogenesis in males. Our results demonstrate that global actions of Mxd3 are central to the initiation of obesity in the gMX zebrafish through their effects on adipogenesis and that MXD3 could serve as a therapeutic target for obesity-associated liver diseases.

show abstract

“…It was discovered that the controlled release of melanocyte-stimulating hormone (MSH) in the hypothalamus can be achieved by administering microparticles through hypothalamic injections [249]. This technique enables the specific targeting of the paraventricular nucleus while preventing the degradation of the peptide.…”

Section: Micro-and Nanoparticlesmentioning

confidence: 99%

Anti-Obesity Drug Delivery Systems: Recent Progress and Challenges

Ashour,

Mabrouk,

Aboelnasr

et al. 2023

Pharmaceutics

View full text Add to dashboard Cite

Obesity has reached an epidemic proportion in the last thirty years, and it is recognized as a major health issue in modern society now with the possibility of serious social and economic consequences. By the year 2030, nearly 60% of the global population may be obese or overweight, which emphasizes a need for novel obesity treatments. Various traditional approaches, such as pharmacotherapy and bariatric surgery, have been utilized in clinical settings to treat obesity. However, these methods frequently show the possibility of side effects while remaining ineffective. There is, therefore, an urgent need for alternative obesity treatments with improved efficacy and specificity. Polymeric materials and chemical strategies are employed in emerging drug delivery systems (DDSs) to enhance therapy effectiveness and specificity by stabilizing and controlling the release of active molecules such as natural ingredients. Designing DDSs is currently a top priority research objective with an eye towards creating obesity treatment approaches. In reality, the most recent trends in the literature demonstrate that there are not enough in-depth reviews that emphasize the current knowledge based on the creation and design of DDSs for obesity treatment. It is also observed in the existing literature that a complex interplay of different physical and chemical parameters must be considered carefully to determine the effectiveness of the DDSs, including microneedles, for obesity treatment. Additionally, it is observed that these properties depend on how the DDS is synthesized. Although many studies are at the animal-study stage, the use of more advanced DDS techniques would significantly enhance the development of safe and efficient treatment approaches for obese people in the future. Considering these, this review provides an overview of the current anti-obesity treatment approaches as well as the conventional anti-obesity therapeutics. The article aims to conduct an in-depth discussion on the current trends in obesity treatment approaches. Filling in this knowledge gap will lead to a greater understanding of the safest ways to manage obesity.

show abstract

Depletion of Alpha-Melanocyte-Stimulating Hormone Induces Insatiable Appetite and Gains in Energy Reserves and Body Weight in Zebrafish

Cited by 10 publications

References 66 publications

Protocol to evaluate hyperlipidemia in zebrafish larvae

Protocol to evaluate hyperlipidemia in zebrafish larvae

MXD3 Promotes Obesity and the Androgen Receptor Signaling Pathway in Gender-Disparity Hepatocarcinogenesis

Anti-Obesity Drug Delivery Systems: Recent Progress and Challenges

Contact Info

Product

Resources

About