Depletion of BIRC6 leads to retarded bovine early embryonic development and blastocyst formation in vitro

Salilew‐Wondim, Dessie; Hölker, Michael; Rings, F.; Phatsara, C.; Mohammadi‐Sangcheshmeh, Abdollah; Tholen, Ernst; Schellander, K.; Tesfaye, Dawit

doi:10.1071/rd09112

Cited by 16 publications

(10 citation statements)

References 56 publications

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“…Previous research has reported polymorphisms in the BIRC6 gene were associated with hereditary eye diseases, pseudoexfoliative glaucoma25. And the mutations of BIRC6 would result in abnormal embryonic development2627. Walsh et al .…”

Section: Discussionmentioning

confidence: 99%

Scanning of selection signature provides a glimpse into important economic traits in goats (Capra hircus)

Guan

Luo

Tan³

et al. 2016

Sci Rep

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Goats (Capra hircus) are one of the oldest livestock domesticated species, and have been used for their milk, meat, hair and skins over much of the world. Detection of selection footprints in genomic regions can provide potential insights for understanding the genetic mechanism of specific phenotypic traits and better guide in animal breeding. The study presented here has generated 192.747G raw data and identified more than 5.03 million single-nucleotide polymorphisms (SNPs) and 334,151 Indels (insertions and deletions). In addition, we identified 155 and 294 candidate regions harboring 86 and 97 genes based on allele frequency differences in Dazu black goats (DBG) and Inner Mongolia cashmere goats (IMCG), respectively. Populations differentiation reflected by Fst values detected 368 putative selective sweep regions including 164 genes. The top 1% regions of both low heterozygosity and high genetic differentiation contained 239 (135 genes) and 176 (106 genes) candidate regions in DBG and IMCG, respectively. These genes were related to reproductive and productive traits, such as “neurohypophyseal hormone activity” and “adipocytokine signaling pathway”. These findings may be conducive to molecular breeding and the long-term preservation of the valuable genetic resources for this species.

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Section: Discussionmentioning

confidence: 99%

Scanning of selection signature provides a glimpse into important economic traits in goats (Capra hircus)

Guan

Luo

Tan³

et al. 2016

Sci Rep

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“…[ 30 ] reported that Apollon affect Smac protein levels in wild-type mice tissues in vivo . Other researchers [ 31 , 32 ] reported that Apollon may also regulate Smac mRNA levels. Based on these results, we proposed that effect of Apollon on Smac may vary according to species and tissue types.…”

Section: Discussionmentioning

confidence: 99%

Apollon modulates chemosensitivity in human esophageal squamous cell carcinoma

et al. 2014

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Patients with esophageal squamous cell carcinoma (ESCC) are often diagnosed with advanced diseases that respond poorly to chemotherapy. Here we reported that Apollon, a membrane-associated inhibitor of apoptosis protein, was overexpressed in ESCC cell lines and clinical ESCC tissues, and Apollon overexpression clinically correlated with poor response to chemotherapy (P = 0.001), and short overall survival (P = 0.021). Apollon knockdown increased cisplatin/docetaxel-induced apoptosis, mitochondrial dysfunction and cytochrome c release in two ESCC cell lines. Apollon knockdown potentiated cisplatin/docetaxel-induced long-term cell growth inhibition, and enhanced chemosensitivity of ESCC cells to cisplatin/docetaxel in xenograft tumor models. Apollon knockdown also enhanced cisplatin/docetaxel-induced activation of caspase-8 (extrinsic pathway) and caspase-9 (intrinsic pathway) in ESCC cells and xenograft tumor models. Mechanism studies revealed that the effect of Apollon on chemosensitivity is mainly mediated by Smac. Apollon expression strongly and negatively correlated with Smac expression in clinical ESCC tissues (P = 0.001). Apollon targeted Smac for degradation in ESCC cells. The effect of Apollon on chemosensitivity was reversed by Smac knockdown in ESCC cells. Taken together, our data show association of Apollon expression with chemotherapeutic response in ESCC, and provide a strong rationale for combining Apollon antagonism with chemotherapy to treat ESCC.

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“…IVP. In vitro fertilization and in vitro culture was performed as described previously (84). In brief, bovine ovaries were obtained from the local abattoir and transported to the laboratory in a thermo flask containing 0.9% saline solution.…”

Section: Methodsmentioning

confidence: 99%

Aberrant placenta gene expression pattern in bovine pregnancies established after transfer of cloned or in vitro produced embryos

Salilew‐Wondim

Tesfaye

Hossain

et al. 2013

Physiological Genomics

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In the present study, we used the global transcriptome profile approach to identify dysregulated genes, molecular pathways, and molecular functional alterations in bovine placentas derived from somatic cell nuclear transfer (SCNT) and in vitro embryo production (IVP) pregnancies compared with their artificial insemination (AI) counterparts at day 50 of gestation. For this, day 7 blastocysts derived from AI, IVP, or SCNT were transferred to oestrus-synchronized cows. The pregnant animals were slaughtered at day 50 of gestation, and the placentas were then recovered and used for transcriptome analysis using Affymetrix GeneChip bovine genome array. Results showed the SCNT placenta to be different from its AI counterpart in the expression of 1,196 transcripts. These genes were found to be associated with alterations in key biological processes and molecular pathways in SCNT placenta, and the dysregulation of 9% (n = 110) of these genes was due to transcriptional reprogramming error. IVP placenta also displayed alterations in the expression of 72 genes, of which 58 were common to SCNT placenta. Gene enrichment analysis revealed that the expression of genes involved in organ development, blood vessel development, extracellular matrix organization, and the immune system was affected in both SCNT and IVP placentas. However, 96% of the affected genes in SCNT were not significantly altered in IVP groups. Thus, the higher transcriptome dysregulation in SCNT placenta followed by IVP would reflect the degree of placental abnormality in SCNT and IVP pregnancies at day 50 of the gestation, which may have a profound effect on subsequent fetal development and health of the offspring.

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Depletion of BIRC6 leads to retarded bovine early embryonic development and blastocyst formation in vitro

Cited by 16 publications

References 56 publications

Scanning of selection signature provides a glimpse into important economic traits in goats (Capra hircus)

Scanning of selection signature provides a glimpse into important economic traits in goats (Capra hircus)

Apollon modulates chemosensitivity in human esophageal squamous cell carcinoma

Aberrant placenta gene expression pattern in bovine pregnancies established after transfer of cloned or in vitro produced embryos

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