Depletion of high-abundance proteins from human plasma using a combination of an affinity and pseudo-affinity column

Urbas, Lidija; Brne, Peter; Gabor, Boštjan; Barut, Miloš; Strlič, Matija; Petrič, Tatjana Čerk; Štrancar, Aleš

doi:10.1016/j.chroma.2008.10.104

Cited by 36 publications

(17 citation statements)

References 18 publications

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“…In comparison to particulate supports where molecules are transferred by diffusion, the high porosity of monoliths allows convective mass transport. This makes resolution and dynamic binding capacity practically independent of the flow rate (24–27). High dynamic binding capacity for large molecules and high flow rates at a very low pressure drop enable rapid processing of large volumes of complex biological mixtures (28).…”

mentioning

confidence: 99%

High Throughput Isolation and Glycosylation Analysis of IgG–Variability and Heritability of the IgG Glycome in Three Isolated Human Populations

Pučić

Knežević

Vidič

et al. 2011

Molecular & Cellular Proteomics

446

320

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All immunoglobulin G molecules carry N-glycans, which modulate their biological activity. Changes in N-glycosylation of IgG associate with various diseases and affect the activity of therapeutic antibodies and intravenous immunoglobulins. We have developed a novel 96-well protein G monolithic plate and used it to rapidly isolate IgG from plasma of 2298 individuals from three isolated human populations. N-glycans were released by PNGase F, labeled with 2-aminobenzamide and analyzed by hydrophilic interaction chromatography with fluorescence detection. The majority of the structural features of the IgG glycome were consistent with previous studies, but sialylation was somewhat higher than reported previously. Sialylation was particularly prominent in core fucosylated glycans containing two galactose residues and bisecting GlcNAc where median sialylation level was nearly 80%. Very high variability between individuals was observed, approximately three times higher than in the total plasma glycome. For example, neutral IgG glycans without core fucose varied between 1.3 and 19%, a difference that significantly affects the effector functions of natural antibodies, predisposing or protecting individuals from particular diseases. Heritability of IgG glycans was generally between 30 and 50%. The individual's age was associated with a significant decrease in galactose and increase of bisecting GlcNAc, whereas other functional elements of IgG glycosylation did not change much with age. Gender was not an important predictor for any IgG glycan. An important observation is that competition between glycosyltransferases, which occurs in vitro, did not appear to be relevant in vivo, indicating that the final glycan structures are not a simple result of competing enzymatic activities, but a carefully regulated outcome designed to meet the prevailing physiological needs.

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mentioning

confidence: 99%

High Throughput Isolation and Glycosylation Analysis of IgG–Variability and Heritability of the IgG Glycome in Three Isolated Human Populations

Pučić

Knežević

Vidič

et al. 2011

Molecular & Cellular Proteomics

446

320

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“…Depletion of IgG using SPG (Faulkner et al 2011;Fu et al 2005) prior to further analysis is common to decrease the complexity of a sample. Different types of matrices are commonly used, such as porous particles, monoliths and affinity membranes (Urbas et al 2009). There are several products on the market using natural, recombinant or even stabilized derivatives of SPA and SPG on affinity media.…”

Section: Utilization Of Full-length Spa and Spg In Protein Purificationmentioning

confidence: 99%

Purification Systems Based on Bacterial Surface Proteins

Boström¹,

Nilvebrant²,

Hober³

2012

Protein Purification

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“…It is believed that the abundance of rarer proteins may be 6-8 orders of magnitude lower compared to highly expressed proteins and, unlike genomics, low abundance proteins cannot be amplifi ed similarly to transcripts (Aebersold and Cravatt 2002 ) . Importantly, the large dynamic range of protein concentrations can somewhat be overcome in order to 'un-mask' rarer proteins by removing highly abundant proteins such as albumin or IgG by affi nity depletion chromatography (Plavina et al 2007 ;Zolotarjova et al 2008 ;Urbas et al 2009 ) . Sample complexity can be further reduced by the application of affi nity chromatography or cell fractionation into specifi c proteome subsets including enzymes, plasma proteins or phosphoproteins (Lee and Lee 2004 ) .…”

Section: Sample Preparationmentioning

confidence: 99%

Proteomic Characterization of Mesenchymal Stem Cell-Like Populations Derived from Various Tissue Types

Mrozik

Xiong

Zilm

et al. 2011

Stem Cells and Cancer Stem Cells, Volume 4

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The evolution of proteomics has led to its application in identifying biomarkers of biological processes and pathways including signal transduction and cell development. Proteomic technologies are increasingly utilized to defi ne the molecular mechanisms controlling mesenchymal stem/stromal cell (MSC) self-renewal, multipotency and fate. Bone marrow-derived MSCs are highly promising candidates in the fi eld of regenerative medicine based on their high proliferative capacity, multilineage differentiation potential and immunomodulatory properties. Recently, equivalent MSC-like populations have also been isolated from adipose, dental and feto-maternal tissues. This chapter discusses the current technologies available for proteomic analysis and the studies performed on tissue-specifi c MSC-like populations to date.

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Depletion of high-abundance proteins from human plasma using a combination of an affinity and pseudo-affinity column

Cited by 36 publications

References 18 publications

High Throughput Isolation and Glycosylation Analysis of IgG–Variability and Heritability of the IgG Glycome in Three Isolated Human Populations

High Throughput Isolation and Glycosylation Analysis of IgG–Variability and Heritability of the IgG Glycome in Three Isolated Human Populations

Purification Systems Based on Bacterial Surface Proteins

Proteomic Characterization of Mesenchymal Stem Cell-Like Populations Derived from Various Tissue Types

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