Depletion of intramuscularly injected procaine penicillin G from tissues of swine. A comparison of HPLC and bioassay procedures

Moats, W. A.; Harris, Elmer W.; Steele, N. C.

doi:10.1021/jf00069a018

Cited by 13 publications

(4 citation statements)

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“…Data for depletion of penicillin residues from swine tissues are scant, particularly in penicillin G procaine salt form used under extra-label usage conditions. Moats et al treated market hogs (80–110 kg) at 13 200 IU/kg (2× label dose) and slaughtered the animals at 4 h, and at 1, 2, 4, and 8 days post treatment, and concluded that penicillin concentrations in muscle were quite variable but did not recommend a preslaughter withdrawal period. A study conducted with 2× and 10× label doses in market pigs (96–104 kg) by Korsrud et al with withdrawal periods of 1, 2, 3, 5, and 7 days provided a foundation for Health Canada to recommend an 8-day withdrawal period for swine treated with a 2× label dose and a 15 day preslaughter withdrawal period for animals treated with a 10× label dose .…”

Section: Introductionmentioning

confidence: 99%

Depletion of Penicillin G Residues in Heavy Sows after Intramuscular Injection. Part I: Tissue Residue Depletion

Lupton

Shelver

Newman

et al. 2014

J. Agric. Food Chem.

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Heavy sows (n = 126) were treated with penicillin G procaine at a 5× label dose (33 000 IU/kg) for 3 consecutive days by intramuscular (IM) injection using three patterns of drug administration. Treatments differed by injection pattern and injection volume. Sets of sows were slaughtered 5, 10, 15, 20, 25, 32, and 39 days after the last treatment; skeletal muscle, kidney, serum, and urine were collected for penicillin G analysis by LC-MS/MS. Penicillin G at withdrawal day 5 averaged 23.5 ± 10.5 and 3762 ± 1932 ng/g in muscle and kidney, respectively. After 15 days of withdrawal, muscle penicillin G residues were quantifiable in only one treated hog (3.4 ng/g) but averaged 119 ± 199 ng/g in kidneys. Using a hypothetical tolerance of 50 ng/g and a natural log-linear depletion model, the withdrawal period required for penicillin depletion to 50 ng/g was 11 days for skeletal muscle and 47 days for kidney.

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Section: Introductionmentioning

confidence: 99%

Depletion of Penicillin G Residues in Heavy Sows after Intramuscular Injection. Part I: Tissue Residue Depletion

Lupton

Shelver

Newman

et al. 2014

J. Agric. Food Chem.

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“…4 Unfortunately, there is little information describing the depletion of penicillin G procaine residues under extra-label conditions. Moats et al 5 measured the depletion of a single intramuscular dose (2× label; 13 200 IU/kg bw) of penicillin G procaine from market hogs. Penicillin residues fell below detectable levels in kidney and muscle by 24 h after injection, and liver residues dropped below detectable limits by 48 h. Moats et al 5 did not report the assay limits of detection, but the lowest penicillin residue reported using HPLC was 0.04 and 0.01 ppm with a microbial inhibition assay.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Moats et al 5 measured the depletion of a single intramuscular dose (2× label; 13 200 IU/kg bw) of penicillin G procaine from market hogs. Penicillin residues fell below detectable levels in kidney and muscle by 24 h after injection, and liver residues dropped below detectable limits by 48 h. Moats et al 5 did not report the assay limits of detection, but the lowest penicillin residue reported using HPLC was 0.04 and 0.01 ppm with a microbial inhibition assay. Korsrud et al 6 administered market hogs IM penicillin G procaine doses exceeding label recommendations by 2× and 10× (15 000 and 66 000 IU/kg, respectively) for 3 (2× dose) or 5 days (10× dose).…”

Section: ■ Introductionmentioning

confidence: 99%

Depletion of Penicillin G Residues in Heavy Sows after Intramuscular Injection. Part II: Application of Kidney Inhibition Swab Tests

Shelver

Lupton

Newman

et al. 2014

J. Agric. Food Chem.

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Sows (n = 126; 228 ± 30.1 kg) were administered daily IM doses of penicillin G procaine (33 000 IU/kg bw; 5× the label dose) for 3 consecutive days using three different administration patterns. Within treatment, six sows each were slaughtered on withdrawal day 5, 10, 15, 20, 25, 32, and 39. Tissues (injection site, kidney, liver, skeletal muscle) or body fluids (serum and urine) were screened for penicillin G using the KIS test, recently adopted by the USDA Food Safety and Inspection Service. The IM administration patterns had no discernible effect on penicillin G depletion. Residues were depleted more rapidly from liver and skeletal muscle and more slowly from kidney and urine. Kidney was the most sensitive and suitable tissue for detecting penicillin G residues on-site, with two positive results after a 39-day withdrawal period. Urine was the most suitable ante-mortem surrogate to predict the results of kidney tests.

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“…To overcome this disadvantage inherent in microbiological methods, chemical analysis such as chromatographic techniques (HPLC) were thus undertaken for macrolide antibiotic analysis [6,7,8,9,10].…”

Section: Introductionmentioning

confidence: 99%

Comparative study of coulometric and amperometric detection for the determination of macrolides in human urine using high-performance liquid chromatography

2003

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A high-performance liquid chromatography (HPLC) method using chromatographic conditions optimised in a previous work was applied for the separation of three macrolide antibiotics roxithromycin (Rox), oleandomycin (Ole) and rosamicin (Ros) and further determination of two of them, roxithromycin (Rox) and oleandomycin (Ole), in human urine samples. A comparative study of the behaviour of these macrolides under the two types of electrochemical detection (EC) widely coupled with HPLC, that is coulometric (EC-C) and amperometric (EC-A), was carried out by applying the same multiresidue method. From the assays performed using both detectors the comparison was made taking relevant criteria such as detection limits, linearity, recovery and precision values into account. As a result of this comparison, the coulometric detector appears slightly more suitable than the amperometric one for macrolide analysis.

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Depletion of intramuscularly injected procaine penicillin G from tissues of swine. A comparison of HPLC and bioassay procedures

Cited by 13 publications

References 1 publication

Depletion of Penicillin G Residues in Heavy Sows after Intramuscular Injection. Part I: Tissue Residue Depletion

Depletion of Penicillin G Residues in Heavy Sows after Intramuscular Injection. Part I: Tissue Residue Depletion

Depletion of Penicillin G Residues in Heavy Sows after Intramuscular Injection. Part II: Application of Kidney Inhibition Swab Tests

Comparative study of coulometric and amperometric detection for the determination of macrolides in human urine using high-performance liquid chromatography

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