Depletion of the paternal gut microbiome alters sperm small RNAs and impacts offspring physiology and behavior in mice

Masson, Bethany A.; Kiridena, Pamudika; Lu, Da; Kleeman, Elizabeth A.; Reisinger, Sonali N.; Qin, Wendy; Davies, William J.; Muralitharan, Rikeish R.; Jama, Hamdi A.; Antonacci, Simona; Marques, Francine Z.; Gubert, Carolina; Hannan, Anthony J.

doi:10.1016/j.bbi.2024.09.020

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Cited by 2 publications

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More than just a number: the gut microbiota and brain function across the extremes of life

Nuzum,

Deady,

Kittel-Schneider

et al. 2024

Gut Microbes

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More than just a number: the gut microbiota and brain function across the extremes of life

Nuzum,

Deady,

Kittel-Schneider

et al. 2024

Gut Microbes

View full text Add to dashboard Cite

Embryonic Signatures of Intergenerational Inheritance across Paternal Environments and Genetic Backgrounds

Dura,

Ranjan,

Paribeni

et al. 2024

Preprint

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The paternal preconception environment has been implicated as a modulator of phenotypic traits and disease risk in F1 offspring. However, the prevalence and mechanisms of such intergenerational epigenetic inheritance (IEI) in mammals remain poorly defined. Moreover, the interplay between paternal exposure, genetics, and age on emergent offspring features is unexplored. Here, we measure the quantitative impact of three paternal environments on early embryogenesis across genetic backgrounds. Using in vitro fertilisation (IVF) at scale, we capture batch-robust transcriptomic signatures of IEI with single-blastocyst resolution. Amongst these, paternal gut microbiota dysbiosis is linked with aberrant expression of lineage regulators in blastocysts, particularly affecting extra-embryonic tissues. Conversely, paternal low-protein high-sugar diet associates with subtle preimplantation developmental delay. We further identify gene expression variability as a paternally-induced F1 phenotype, and highlight confounding issues for IEI such as batch-effects and under-sampling. Finally, paternal genetic background and age exert a dominant influence over the inherited environmental signature. This study systematically characterises how paternal conditioning programmes subtle but detectable molecular responses in early embryos, and suggests guiding principles to dissect intergenerational phenomenology.

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Depletion of the paternal gut microbiome alters sperm small RNAs and impacts offspring physiology and behavior in mice

Cited by 2 publications

References 76 publications

More than just a number: the gut microbiota and brain function across the extremes of life

More than just a number: the gut microbiota and brain function across the extremes of life

Embryonic Signatures of Intergenerational Inheritance across Paternal Environments and Genetic Backgrounds

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