The article deals with the complex dynamics of depopulation in rural areas in Serbia. Although the topic has been very topical in recent decades and research has long identified the causes and consequences of depopulation in numerous regions in Europe, there seems to be no systematic approach to the implementation of policies for the management of abandoned areas. We started from already known factors contributing to depopulation, including economic, social and environmental dimensions. Based on previous research, historical trends and a statistical database, we attempted to represent depopulation as a spatial process that has its own size, structure and consequences, using a new, quantitative methodology for grouping abandoned areas into clusters as basic units of dispersal of depopulated areas. The aim of the work was to quantitatively determine the size and structure of the depopulated area in Serbia after the 2022 census. The study opened up new fields of research for the future use of depopulated space, but also offered certain solutions for policy makers, researchers and municipalities struggling with the challenges of depopulation. The results of the work show the existence of 47 abandoned clusters in 10 administrative regions of Serbia, which is 17 more than in the 2011 census. Further homogenization and consolidation of clusters was found in the statistical regions: Toplička, Zaječarska, Jablanička, Pirotska and Raška region, i.e. an increase in the total depopulated area as well as its spatial expansion towards the north of the country.