Deposition of Cellulose Nanocrystals onto Supported Lipid Membranes

Abstract

The deposition of cellulose nanocrystals (CNCs) on a supported lipid bilayer (SLB) was investigated at different length scales. Quartz crystal microbalance with dissipation monitoring (QCM-D) was used to probe the bilayer formation and to show for the first time the CNC deposition onto the SLB. Specifically, classical QCM-D measurements gave estimation of the adsorbed hydrated mass and the corresponding film thickness, whereas complementary experiments using D2O as the solvent allowed the quantitative determination of the hydration of the CNC layer, showing a high hydration value. Scanning force microscopy (SFM) and total internal reflection fluorescence microscopy (TIRF) were used to probe the homogeneity of the deposited layers, revealing the structural details at the particle and film length scales, respectively, thus giving information on the effect of CNC concentration on the surface coverage. The results showed that the adsorption of CNCs on the supported lipid membrane depended on lipid composition, CNC concentration, and pH conditions, and that the binding process was governed by electrostatic interactions. Under suitable conditions, a uniform film was formed, with thickness corresponding to a CNC monolayer, which provides the basis for a relevant 2D model of a primary plant cell wall.

Adsorption of a fabric conditioner on cellulose nanocrystals: synergistic effects of surfactant vesicles and polysaccharides on softness properties

“…These data, compared with those obtained from cryo-TEM experiments in Fig. 1b, suggest that the layers consist primarily of side-on lying crystallites, arranged with the longitudinal axes parallel to the surface (Navon et al 2020). It should be noted that the coating locally exhibits correlations in the CNC orientations.…”

“…CNCs and vesicles have been studied in bulk dispersions however. Navon et al (Navon et al 2017) analyzed the mixtures of CNC-1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) vesicles in aqueous bulk solutions to understand the interaction between cellulose and a cell membrane mimicking system (Navon et al 2020). In addition, this group demonstrated by cryo-TEM that the vesicles remain stable during CNC adsorption.…”

Interaction of a Phospholipid and a Coagulating Protein: Potential Candidate for Bioelectronic Applications

“…It is worth noting that DOPC is a zwitterionic lipid with a positively charged choline group and a negatively charged phosphate acid group. 78 , 79 Polar head groups of DOPC can reorient depending on the ionic strength. 80 , 81 At low ionic strength, the choline groups are located below the phosphate groups, whereas at higher ionic strength, the situation is reversed.…”

Release of Cellulose Nanocrystal Particles from Natural Rubber Latex Composites into Immersed Aqueous Media

“…Proteins and lipids that cover NR latex particles are believed to occupy a separate phase between NR latex particles. CNCs have weak interactions with cis -1,3 polyisoprene molecules of NR latex particles; however, stronger interactions between proteins and CNCs, , as well as the interaction between lipids and CNCs, have been widely reported. CNC particles are not visible in Figure ; they most likely stayed within the protein and lipid components.…”