Deposition of nanolayers by means of dense plasma focus

Chernyshova, M.; Ivanova‐Stanik, I.; Karpinski, L.; Scholz, M.; Ulejczyk, Bogdan; Demchenko, I.N.; Lee, J. K.

doi:10.1007/s10582-006-0205-4

Cited by 8 publications

(1 citation statement)

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“…The specimens were fitted into the holder in an offcenter position (20 • -40 • ) to the anode axis. The specimen holder was placed axially in front of the anode at a distance of 130 mm from the anodic gun [39,40]. The graphite solid rod (purity > 99.9%), was fitted in the center of the hollow anode tip to act as a precursor for the deposition of NDs on the PCL scaffold specimens.…”

Section: Nds Surface Deposition On 3d-printed Pcl Scaffold Specimensmentioning

confidence: 99%

Effect of nanodiamonds surface deposition on hydrophilicity, bulk degradation and in-vitro cell adhesion of 3D-printed polycaprolactone scaffolds for bone tissue engineering

ElBakry,

Ammar,

Moussa

2024

Biomed. Mater.

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This study was designed to deposit nanodiamonds on 3D-printed PCL scaffolds and evaluate their effect on the surface topography, hydrophilicity, degradation, and in-vitro cell adhesion compared to untreated PCL scaffolds. The PCL scaffold specimens were 3D-printed by fused deposition molding (FDM) technique with specific porosity parameters. The 3D-printed specimens' surfaces were modified by nanodiamonds deposition followed by oxygen plasma post-treatment using a plasma focus (PF) device and a non-thermal atmospheric plasma jet (NTAPJ), respectively. Specimens were evaluated through morphological characterization by field emission scanning electron microscope (FESEM), microstructure characterization by Raman spectroscopy, chemical characterization by Fourier transform infrared (FTIR) spectroscopy, hydrophilicity degree by contact angle and water uptake measurements, and in-vitro degradation measurements (n=6). In addition, in-vitro bone marrow mesenchymal stem cells (BMSCs) adhesion was evaluated quantitatively by Confocal microscopy and qualitatively by FESEM at different time intervals after cell seeding (n=6). The statistical significance level was set at p ≤0.05. The FESEM micrographs, the Raman, and FTIR spectra confirmed the successful surface deposition of nanodiamonds on scaffold specimens. The nanodiamonds treated specimens showed nano-scale features distributed homogeneously across the surface compared to the untreated ones. Also, the nanodiamonds treated specimens revealed a statistically significant smaller contact angle (17.45 ±1.34 degrees), higher water uptake percentage after 24 h immersion in phosphate buffer saline (PBS) (21.56% ±1.73), and higher degradation rate after six months of immersion in PBS (43.92% ±0.77). Moreover, enhanced cell adhesion at all different time intervals was observed in nanodiamonds treated specimens with higher nuclei area fraction percentage (69.87% ±3.97) compared to the untreated specimens (11.46% ±1.34). Surface deposition of nanodiamonds with oxygen-containing functional groups on 3D-printed PCL scaffolds increased their hydrophilicity and degradation rate with significant enhancement of the in-vitro cell adhesion compared to untreated PCL scaffolds.

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Section: Nds Surface Deposition On 3d-printed Pcl Scaffold Specimensmentioning

confidence: 99%