“…The main issue is that pollen analyses requires a minimum ice sample size of up to 1 L (e.g., Burogois, 2000), which is problematic to obtain because sample volume from ice cores is very limited, especially when working at high resolution. However, studies from the Altai (Nakazawa et al, 2015(Nakazawa et al, , 2011(Nakazawa et al, , 2005(Nakazawa et al, , 2004 and the European Alps (Festi et al, 2015;Bortenschlager, 1970a, b;Vareschi et al, 1934) suggest that this is still a limit for clean samples obtained from polar ice caps, whereas in low and midlatitude glaciers, the minimum sample size can be reduced to 10-30 mL (Festi et al, 2015;Nakazawa et al, 2011Nakazawa et al, , 2005 thanks to the proximity of the source vegetation and the consequent much greater pollen deposition. An additional limit is that pollen analyses are time consuming and work intensive because they imply manual identification and quantification of pollen grains.…”