Depot-specific adipocyte-extracellular matrix metabolic crosstalk in murine obesity

Strieder‐Barboza, Clarissa; Baker, Nicki A.; Flesher, Carmen G.; Karmakar, Monita; Patel, Ayush D.; Lumeng, Carey N.; O’Rourke, Robert W.

doi:10.1080/21623945.2020.1749500

Cited by 23 publications

(18 citation statements)

References 29 publications

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“…In humans, visceral adipose tissue is associated with insulin resistance, dyslipidaemia and Type 2 diabetes, while subcutaneous adipose tissue is associated with preserved insulin sensitivity and mitigates risk of metabolic disorders [30][31][32]. Moreover, in vitro study revealed that adipocytes from visceral adipose tissue rather than subcutaneous adipose tissue of obese mice showed impaired insulin-stimulated glucose uptake, adipogenesis ability and ECM remodelling representing dysfunctional adipocytes [33]. Thus, our result that Col24α1 mRNA expression was increased in the visceral adipose tissue of obese and diabetic subjects but not in the subcutaneous adipose tissue implied a potential pathogenic role of Col24α1 in obesity and Type 2 diabetes.…”

Section: Discussionmentioning

confidence: 99%

Collagen 24 α1 Is Increased in Insulin-Resistant Skeletal Muscle and Adipose Tissue

Weng

Lin

Huang

et al. 2020

IJMS

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Aberrant extracellular matrix (ECM) remodelling in muscle, liver and adipose tissue is a key characteristic of obesity and insulin resistance. Despite its emerging importance, the effective ECM targets remain largely undefined due to limitations of current approaches. Here, we developed a novel ECM-specific mass spectrometry-based proteomics technique to characterise the global view of the ECM changes in the skeletal muscle and liver of mice after high fat (HF) diet feeding. We identified distinct signatures of HF-induced protein changes between skeletal muscle and liver where the ECM remodelling was more prominent in the muscle than liver. In particular, most muscle collagen isoforms were increased by HF diet feeding whereas the liver collagens were differentially but moderately affected highlighting a different role of the ECM remodelling in different tissues of obesity. Moreover, we identified a novel association between collagen 24α1 and insulin resistance in the skeletal muscle. Using quantitative gene expression analysis, we extended this association to the white adipose tissue. Importantly, collagen 24α1 mRNA was increased in the visceral adipose tissue, but not the subcutaneous adipose tissue of obese diabetic subjects compared to lean controls, implying a potential pathogenic role of collagen 24α1 in obesity and type 2 diabetes.

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Section: Discussionmentioning

confidence: 99%

Collagen 24 α1 Is Increased in Insulin-Resistant Skeletal Muscle and Adipose Tissue

Weng

Lin

Huang

et al. 2020

IJMS

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“… 3 Visceral adipose tissue in human is more closely associated with metabolic disease than subcutaneous adipose tissue. 4 The rate of obesity has almost tripled globally in the last decades, with an estimated 1.9 billion adults overweight. 5 Although obesity has become a pandemic and a major burden in one of the high-income countries in Kingdom of Saudi Arabia.…”

Section: Introductionmentioning

confidence: 99%

Relationship Between Serum Amyloid A1 (SAA1) Gene Polymorphisms Studies with Obesity in the Saudi Population

Alharbi¹,

Alshammary²,

Aljabri³

et al. 2021

DMSO

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Purpose: Limited studies have shown positive and negative associations of serum amylase A1 (SAA1) gene in childhood obesity, previously showed the relation with obesity in different ethnicity. The current study therefore investigated the impact of single nucleotide polymorphisms present in the SAA1 gene on subjects of Saudi obesity. Participants and Methods: In this case-control study, we selected 140 subjects of Saudi population and categorized them into 83 cases of obesity and 57 healthy controls. Genotyping was performed with quantitative/real time-polymerase chain reaction in the SAA1 gene for rs11603089A/G, rs4638289A/T and rs7131332A/G polymorphisms. Results: In rs11603089 polymorphism, co-dominant model (AG vs AA+GG; OR-2.23 [95% CI:1.02-4.86]; p=0.04) and rs4638289 polymorphism, a disparity in significance was observed between the homozygous variant (TT vs AA; OR-16.8 [95% CI: 2.06-136.8]; p=0.0009), dominant model (AT+TT vs AA; OR-2.57 [95% CI: 1.28-5.19]; p=0.007), recessive model (TT vs AA+AT; OR-11.36 [95% CI: 1.45-89.06]; p=0.004) and allelic frequency for (T vs A: OR-2.35 [95% CI: 1.39-3.98]; p=0.001) between the obesity cases and control subjects. However, statistical correlations did not reveal the rs7131332A/G polymorphism either (p>0.05). Conclusion:In conclusion, rs4638289 polymorphism was associated with risk allele and dominant model with obesity subjects. Further additional studies were warranted.

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“…Recently, high levels of LAMB1 were observed in visceral adipose tissue and during in vitro adipogenesis. Obesity alters the gene expression profile of ECM genes in adipose tissue (LAMB1 was upregulated in visceral and subcutaneous adipose tissue) [57][58][59][60]. The lactate dehydrogenase A protein (LDHA) catalyzes the conversion of pyruvate to lactate in the glycolytic metabolism.…”

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confidence: 99%

Proteogenomic Analysis Reveals Proteins Involved in the First Step of Adipogenesis in Human Adipose-Derived Stem Cells

Bonilauri

Camillo-Andrade

Santos

et al. 2021

Stem Cells International

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Background. Obesity is characterized as a disease that directly affects the whole-body metabolism and is associated with excess fat mass and several related comorbidities. Dynamics of adipocyte hypertrophy and hyperplasia play an important role in health and disease, especially in obesity. Human adipose-derived stem cells (hASC) represent an important source for understanding the entire adipogenic differentiation process. However, little is known about the triggering step of adipogenesis in hASC. Here, we performed a proteogenomic approach for understanding the protein abundance alterations during the initiation of the adipogenic differentiation process. Methods. hASC were isolated from adipose tissue of three donors and were then characterized and expanded. Cells were cultured for 24 hours in adipogenic differentiation medium followed by protein extraction. We used shotgun proteomics to compare the proteomic profile of 24 h-adipogenic, differentiated, and undifferentiated hASC. We also used our previous next-generation sequencing data (RNA-seq) of the total and polysomal mRNA fractions of hASC to study posttranscriptional regulation during the initial steps of adipogenesis. Results. We identified 3420 proteins out of 48,336 peptides, of which 92 proteins were exclusively identified in undifferentiated hASC and 53 proteins were exclusively found in 24 h-differentiated cells. Using a stringent criterion, we identified 33 differentially abundant proteins when comparing 24 h-differentiated and undifferentiated hASC (14 upregulated and 19 downregulated, respectively). Among the upregulated proteins, we shortlisted several adipogenesis-related proteins. A combined analysis of the proteome and the transcriptome allowed the identification of positive correlation coefficients between proteins and mRNAs. Conclusions. These results demonstrate a specific proteome profile related to adipogenesis at the beginning (24 hours) of the differentiation process in hASC, which advances the understanding of human adipogenesis and obesity. Adipogenic differentiation is finely regulated at the transcriptional, posttranscriptional, and posttranslational levels.

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Depot-specific adipocyte-extracellular matrix metabolic crosstalk in murine obesity

Cited by 23 publications

References 29 publications

Collagen 24 α1 Is Increased in Insulin-Resistant Skeletal Muscle and Adipose Tissue

Collagen 24 α1 Is Increased in Insulin-Resistant Skeletal Muscle and Adipose Tissue

Relationship Between Serum Amyloid A1 (SAA1) Gene Polymorphisms Studies with Obesity in the Saudi Population

Proteogenomic Analysis Reveals Proteins Involved in the First Step of Adipogenesis in Human Adipose-Derived Stem Cells

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