Depressant Effects of Ambroxol and Erdosteine on Cytokine Synthesis, Granule Enzyme Release, and Free Radical Production in Rat Alveolar Macrophages Activated by Lipopolysaccharide

Jang, Yoon Young; Song, Jin Ho; Shin, Yong Kyoo; Han, Eun Sook; Lee, Chung Soo

doi:10.1034/j.1600-0773.2003.920407.x

Cited by 48 publications

(42 citation statements)

References 35 publications

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“…Similarly, an erdosteine metabolite was reported to react with ROS, NO, and NO-derived peroxynitrite and exert both antioxidant and scavenging activities [46]. Moreover, erdosteine inhibits cytokine synthesis and free radical production in rat alveolar macrophages by effectively decomposing NO [47].…”

Section: Discussionmentioning

confidence: 97%

Erdosteine Prevents Colonic Inflammation Through Its Antioxidant and Free Radical Scavenging Activities

et al. 2007

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After intracolonic administration of trinitrobenzene sulphonic acid (TNBS), Sprague-Dawley rats were treated orally either with saline or erdosteine (100 mg/kg per day), a sulfhydryl-containing antioxidant, for 3 days. On the 4th day, rats were decapitated and distal colon was removed for the macroscopic and microscopic damage scoring, for the measurement of malondialdehyde (MDA), glutathione (GSH) and collagen levels, myeloperoxidase (MPO) activity, luminol and lucigenin chemiluminescence (CL) and DNA fragmentation. Lactate dehydrogenase (LDH) activity, tumor necrosis factor-alpha, interleukin (IL)-1beta, IL-6, and antioxidant capacity were assayed in blood samples. Colitis caused significant increases in the colonic CL values, macroscopic and microscopic damage scores, MDA and collagen levels, MPO activity and DNA fragmentation, along with a significant decrease in tissue GSH level. Similarly, serum cytokines and LDH were elevated in the saline-treated colitis group as compared with the control group. On the other hand, erdosteine treatment reversed all these biochemical indices, and histopathologic alterations induced by TNBS, suggesting that erdosteine protects the colonic tissue via its radical scavenging and antioxidant activities.

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Section: Discussionmentioning

confidence: 97%

Erdosteine Prevents Colonic Inflammation Through Its Antioxidant and Free Radical Scavenging Activities

et al. 2007

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“…Ambroxol has many biological actions on various cell types. Jang et al [27] reported that ambroxol significantly reduced the production of superoxide anion, hydrogen peroxide, and nitric oxide and the release of acid phosphatase and lysozyme in LPS-activated macrophages. Yang et al [20] reported that ambroxol transiently suppressed release of the cytokines TNFa, interferon g and IL-12 into airway fluid.…”

Section: Discussionmentioning

confidence: 98%

Ambroxol reduces LPS toxicity mediated by induction of alkaline phosphatases in rat lung

Koyama

Matsunaga

Harada

et al. 2004

Clinical Biochemistry

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“…Jang et al [21] reported that erdosteine inhibited the synthesis of cytokines including tumor necrosis factor (TNF)-α, IL-1β, and IL-6, and free radical (superoxide, hydrogen peroxide) production in rat alveolar macrophages activated by LPS. They suggest that the inhibitory effect of erdosteine on inflammatory cytokine synthesis and ROS production contributes to the anti-inflammatory effect of erdosteine.…”

Section: Discussionmentioning

confidence: 99%

Anti-inflammatory Effect of Erdosteine in Lipopolysaccharide-Stimulated RAW 264.7 Cells

et al. 2016

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Erdosteine is widely used as a mucolytic agent and also has free radical scavenging and antioxidant activities. However, little is known about the mechanisms of the anti-inflammatory effect of erdosteine. We investigated the effect of erdosteine on the activation of the nuclear factor (NF)-kB/inhibitor of NFkB (IkB), and the mitogen-activated protein kinase (MAPK) and Akt pathways in the mouse macrophage cell line RAW 264.7. Cultured RAW 264.7 cells were pretreated with erdosteine and stimulated with lipopolysaccharide (LPS). In Western blotting, pretreatment with erdosteine inhibited the IkBα degradation induced in RAW 264.7 cells by LPS. LPS-induced IkB kinase (IKK) activity and NF-kB transcription were inhibited by pretreatment with erdosteine. Production of IL-6 and IL-1β was also inhibited by erdosteine pretreatment. However, erdosteine did not inhibit LPS-induced phosphorylation of Akt and MAPKs. These results suggest that the anti-inflammatory effect of erdosteine in mouse macrophages is mediated through inhibition of LPS-induced NF-kB activation.

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Depressant Effects of Ambroxol and Erdosteine on Cytokine Synthesis, Granule Enzyme Release, and Free Radical Production in Rat Alveolar Macrophages Activated by Lipopolysaccharide

Cited by 48 publications

References 35 publications

Erdosteine Prevents Colonic Inflammation Through Its Antioxidant and Free Radical Scavenging Activities

Erdosteine Prevents Colonic Inflammation Through Its Antioxidant and Free Radical Scavenging Activities

Ambroxol reduces LPS toxicity mediated by induction of alkaline phosphatases in rat lung

Anti-inflammatory Effect of Erdosteine in Lipopolysaccharide-Stimulated RAW 264.7 Cells

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